Preparation method for spirulina polymer composite tissue engineering scaffold

Abstract

The invention discloses a preparation method for a spirulina polymer composite tissue engineering scaffold. The preparation method comprises the following steps: dissolving spirulina crude extract and polymer into an organic solvent in a mass ratio being 1: (2-20) to prepare a mixed solution with mass concentration being 10-25%, stirring for 24-48 hours, and uniformly mixing with a pore-foaming agent 7-10 times the mass of solid of the mixed solution; then, pouring the mixture into a mould with a needed shape to be subjected to room-temperature air-drying for 24-72 hours, drying for 24-48 hours at a temperature of 50-70 DEG C under vacuum degree of 0.001-0.01Mpa, taking out a sample in the mould and soaking the sample into deionized water, replacing the deionized water every 4-8 hours, getting out the sample after replacing for 18-40 times in total; and drying the sample for 24-48 hours at a temperature of 40-70 DEG C under vacuum degree of 0.001-0.01MPa. The spirulina polymer composite tissue engineering scaffold obtained by the method has the characteristics of being good in hydrophily, high in compression strength, quick to degrade and non-toxic.

Description

technical field [0001] The invention relates to a preparation method of a spirulina polymer composite tissue engineering scaffold, in particular to a preparation method of a spirulina polymer composite tissue engineering scaffold with good hydrophilicity, rapid degradation and non-toxicity. Background technique [0002] Large-scale defects and dysfunction of human tissues caused by trauma, tumors and other factors require emerging tissue engineering scaffolds to provide effective repair for organ (skin, blood vessel, bone, nerve, cartilage, tendon) damage. [0003] In the design and selection of tissue engineering scaffold materials, degradable natural polymer materials, synthetic polymer materials, and high-strength inorganic materials have made outstanding contributions to promoting normal tissue growth (cell growth, reproduction, differentiation) and remodeling. contribution. In recent years, with the continuous advancement of tissue engineering technology, the const...

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Problems solved by technology

However, the spirulina composite tissue engineering scaffold prepared by electrospinning has the defects of low production efficiency and low content of spirulina, especially for the preparation of spirulina composite tissue with larger pore size.

In addition, the spirulina polymer composite engineering scaffold prepared by electrospinning method has very limited hydrophilicity and degradability because the content of spirulina is not easy to be too high.

[0007] In the above and related literature descriptions, there is no literature report that Spirulina has greatly improved the hydrophilicity of tissue engineering scaffolds, and the existing highest content of Spirulina in Spirulina polymer composite tissue engineering scaffolds is only up to 6% polymer composite tissue engineering scaffold material without higher spirulina content

In addition, although many literatures mention that the degradation rate of tissue engineering scaffolds prepared by compounding spirulina and polymers with good biocompatibility and good degradability will be accelerated, but there is no literature on the spirulina polymer composite tissue engineering scaffolds. Conduct in vitro degradation experiments

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