Preparation method for spirulina polymer composite tissue engineering scaffold

A composite tissue and polymer technology, applied in medical science, prosthesis, etc., can solve the problems of hydrophilicity, limited degradability, low production efficiency, low content of spirulina, etc., and achieve the advantages of hydrophilicity and degradability, Improved hydrophilicity and good hydrophilicity

Inactive Publication Date: 2016-08-10
GUANGDONG UNIV OF PETROCHEMICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the spirulina composite tissue engineering scaffold prepared by electrospinning has the defects of low production efficiency and low content of spirulina, especially for the preparation of spirulina composite tissue with larger pore size.
In addition, the spirulina polymer composite engineering scaffold prepared by electrospinning method has very limited hydrophilicity and degradability because the content of spirulina is not easy to be too high.
[0007] In the above and related literature descriptions, there is no literature report that Spirulina has greatly improved the hydrophilicity of tissue engineering scaffolds, and the existing highest content of Spirulina in Spirulina polymer composite tissue engineering scaffolds is only up to 6% polymer composite tissue engineering scaffold material without higher spirulina content
In addition, although many literatures mention that the degradation rate of tissue engineering scaffolds prepared by compounding spirulina and polymers with good biocompatibility and good degradability will be accelerated, but there is no literature on the spirulina polymer composite tissue engineering scaffolds. Conduct in vitro degradation experiments

Method used

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  • Preparation method for spirulina polymer composite tissue engineering scaffold
  • Preparation method for spirulina polymer composite tissue engineering scaffold
  • Preparation method for spirulina polymer composite tissue engineering scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Add 0.1 g of the crude extract of spirulina and 1.9 g of polycaprolactone to acetic acid to prepare a solution with a concentration of 10%, mix it with 14 g of NaCl after stirring for 48 hours, and then pour it into a mold of the desired shape through After air-drying at room temperature for 24 hours, dry for 48 hours at a vacuum degree of 0.01MPa and a temperature of 50°C, take out the sample and immerse it in deionized water, and replace the deionized water for a total of 18 times every 4 hours. The obtained spirulina polymer tissue engineering porous scaffold material is 0.001 Under MPa, vacuum-dry at 50°C for 24h and store in a vacuum desiccator.

[0023] The crude extract of spirulina is prepared by the following method: the dry powder of spirulina that has been dried, crushed, and passed through an 80-mesh sieve is mixed with deionized water at a mass ratio of 10:90, dissolved at 55°C for 6 hours and cooled to room temperature. The solution was centrifuged at 5000...

Embodiment 2

[0025] Add 0.2g of the crude extract of spirulina and 1.8g of polycaprolactone to 11.3g of acetic acid, stir for 48 hours and mix with 14g of NaCl, then pour it into a mold of the desired shape and air-dry it at room temperature for 24 hours , and then dried for 48 hours at a vacuum degree of 0.01MPa and a temperature of 50°C, took out the sample and immersed it in deionized water, and replaced the deionized water every 4h for a total of 18 times. Store in a vacuum desiccator after vacuum drying for 24 h.

Embodiment 3

[0031] Dissolve 0.2g of spirulina crude extract and 1.8g of polycaprolactone in acetic acid to prepare a solution with a mass concentration of 15%, mix it with 16g of NaCl after stirring for 48 hours, and then pour it into a mold of the desired shape After air-drying at room temperature for 24 hours, dry for 48 hours at a vacuum degree of 0.01MPa and a temperature of 50°C, take out the sample and immerse it in deionized water, and replace the deionized water every 4 hours for a total of 18 times. The obtained spirulina polymer porous scaffold material is 0.001 Under MPa, vacuum-dry at 50°C for 24h and store in a vacuum desiccator.

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Abstract

The invention discloses a preparation method for a spirulina polymer composite tissue engineering scaffold. The preparation method comprises the following steps: dissolving spirulina crude extract and polymer into an organic solvent in a mass ratio being 1: (2-20) to prepare a mixed solution with mass concentration being 10-25%, stirring for 24-48 hours, and uniformly mixing with a pore-foaming agent 7-10 times the mass of solid of the mixed solution; then, pouring the mixture into a mould with a needed shape to be subjected to room-temperature air-drying for 24-72 hours, drying for 24-48 hours at a temperature of 50-70 DEG C under vacuum degree of 0.001-0.01Mpa, taking out a sample in the mould and soaking the sample into deionized water, replacing the deionized water every 4-8 hours, getting out the sample after replacing for 18-40 times in total; and drying the sample for 24-48 hours at a temperature of 40-70 DEG C under vacuum degree of 0.001-0.01MPa. The spirulina polymer composite tissue engineering scaffold obtained by the method has the characteristics of being good in hydrophily, high in compression strength, quick to degrade and non-toxic.

Description

technical field [0001] The invention relates to a preparation method of a spirulina polymer composite tissue engineering scaffold, in particular to a preparation method of a spirulina polymer composite tissue engineering scaffold with good hydrophilicity, rapid degradation and non-toxicity. Background technique [0002] Large-scale defects and dysfunction of human tissues caused by trauma, tumors and other factors require emerging tissue engineering scaffolds to provide effective repair for organ (skin, blood vessel, bone, nerve, cartilage, tendon) damage. [0003] In the design and selection of tissue engineering scaffold materials, degradable natural polymer materials, synthetic polymer materials, and high-strength inorganic materials have made outstanding contributions to promoting normal tissue growth (cell growth, reproduction, differentiation) and remodeling. contribution. In recent years, with the continuous advancement of tissue engineering technology, the const...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/18A61L27/54A61L27/56A61L27/58
CPCA61L27/18A61L27/3637A61L27/54A61L27/56A61L27/58A61L2300/30A61L2300/412C08L67/04
Inventor 史博梁亮郭永俊陈秋童
Owner GUANGDONG UNIV OF PETROCHEMICAL TECH
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