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A kind of method utilizing aspergillus niger to degrade zearalenone

A technology for zearalenone and Aspergillus niger is applied in the field of microorganisms and achieves the effects of low cost, simple preparation and application methods, and convenient operation

Active Publication Date: 2019-11-05
湖南微草生物科技有限公司
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  • Claims
  • Application Information

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Problems solved by technology

Existing pure enzyme preparations that can degrade zearalenone have not been reported yet. It is necessary to dig deep into the enzymes and related genes that degrade zearalenone, optimize its fermentation production conditions, and improve its degradation activity and efficiency. and the basic premise of applying

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  • A kind of method utilizing aspergillus niger to degrade zearalenone
  • A kind of method utilizing aspergillus niger to degrade zearalenone
  • A kind of method utilizing aspergillus niger to degrade zearalenone

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Embodiment Construction

[0027] The present invention utilizes Aspergillus niger to degrade a method for zearalenone, and its degradation method is as follows:

[0028] A. Preparation of spore suspension:

[0029] Streak the Aspergillus niger strain on the Chapei high-salt medium plate, culture it in a 30°C incubator for 5 days, use a sterile inoculation loop to pick up the black spores on the aerial hyphae, and inoculate the spores into a mL of sterile water, and finally diluted to 1×10 by serial dilution and hemocytometer counting 7 Concentration of CFU / mL;

[0030] B. Prescription of inorganic salt medium:

[0031] The main components of the medium are: 1L ultrapure water contains: 20-40 g sucrose, K 2 HPO 4 •3H 2 O 0.5-1.5 g, NH 4 Cl 2-4 g, KCl 0.5-1 g, MgSO 4 •7H 2 O 0.25-0.75 g, FeSO 4 0.01-0.02 g, ZnSO 4 •7H 2 O0.075-0.125 g, CuSO 4 • 5H 2 O 0.01-0.03 g, Na 2 MoO 4 • 2H 2 O 0.075-0.125g;

[0032] The best prescription of culture medium: 1L ultrapure water contains: 30 g sucrose...

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Abstract

The invention relates to the field of microorganisms, in particular to a method for degrading zearalenone by using Aspergillus niger. The steps are: A. preparation of spore suspension, B. optimization of inorganic salt medium, and C. degradation of zearalenone. The pure culture cultivated by the method can be used to degrade the mycotoxin zearalenone polluted in various feeds, the degradation rate reaches 99%, and the preparation and application method is simple and the operation is convenient. Provides an effective method for removing mycotoxins (zearalenone) from animal feed.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a method for degrading zearalenone by using Aspergillus niger. Background technique [0002] Zearalenone (zearalenone), also known as F-2 toxin, was first contaminated by Fusarium graminearum ( Fusarium graminearum ) mycotoxin isolated from moldy corn. Zearalenone is mainly produced by Fusarium fungi such as Fusarium graminearum, Fusarium pink ( Fusarium roseum ), Fusarium moniliforme ( Fusarium maniliborme ), three-line Fusarium ( Fusarium tricinctum ) and other synthetic and secreted secondary metabolites are reproductive system toxins with strong teratogenic effects, which can cause serious consequences such as livestock reproductive disorders, abortion and stillbirth, immunosuppression, and decreased growth rate, bringing huge losses to the animal husbandry industry. harm. Because the fungi of the genus Fusarium have strong ecological adaptability and can live both paras...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A23L5/20A23K10/18C12R1/685
CPCC12N1/145C12R2001/685
Inventor 姜旋张素琴罗永祥胡智鹏许加娟黎明张世敏康汇然叶维民贾艳林王跃球魏友岗高遵波梁汉新周桂香潘沼羽朱瑶文少怀李林蒋宇康建南
Owner 湖南微草生物科技有限公司
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