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Method for degrading zearalenone through aspergillus niger

A technology for zearalenone and Aspergillus niger, which is applied in the field of microorganisms and achieves the effects of simple preparation and application methods, convenient operation and low cost

Active Publication Date: 2016-08-10
湖南微草生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Existing pure enzyme preparations that can degrade zearalenone have not been reported yet. It is necessary to dig deep into the enzymes and related genes that degrade zearalenone, optimize its fermentation production conditions, and improve its degradation activity and efficiency. and the basic premise of applying

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  • Method for degrading zearalenone through aspergillus niger
  • Method for degrading zearalenone through aspergillus niger
  • Method for degrading zearalenone through aspergillus niger

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Embodiment Construction

[0027] The present invention utilizes Aspergillus niger to degrade a method for zearalenone, and its degradation method is as follows:

[0028] A. Preparation of spore suspension:

[0029] Streak the Aspergillus niger strain on the Chapei high-salt medium plate, culture it in a 30°C incubator for 5 days, use a sterile inoculation loop to pick up the black spores on the aerial hyphae, and inoculate the spores into a mL of sterile water, and finally diluted to 1×10 by serial dilution and hemocytometer counting 7 Concentration of CFU / mL;

[0030] B. Prescription of inorganic salt medium:

[0031] The main components of the medium are: 1L ultrapure water contains: 20-40 g sucrose, K 2 HPO 4 •3H 2 O 0.5-1.5 g, NH 4 Cl2-4 g, KCl 0.5-1 g, MgSO 4 •7H 2 O 0.25-0.75 g, FeSO 4 0.01-0.02 g, ZnSO 4 •7H 2 O 0.075-0.125 g, CuSO 4 • 5H 2 O 0.01-0.03 g, Na 2 MoO 4 • 2H2 O 0.075-0.125g;

[0032] The best prescription of culture medium: 1L ultrapure water contains: 30 g sucrose...

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Abstract

The invention relates to the field of microbes and especially relates to a method for degrading zearalenone through aspergillus niger. The method comprises A, spore fluid suspension preparation, B, inorganic salt medium optimization and C, zearalenone degradation. A pure culture obtained through the method can be used for degrading fungaltoxin zearalenone pollutants in various types of feed and has a degradation rate of 99%. The method has simple processes and is convenient for operation. The method provides an effective method for removing fungaltoxin zearalenone in animal feed.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a method for degrading zearalenone by using Aspergillus niger. Background technique [0002] Zearalenone, also known as F-2 toxin, is a mycotoxin isolated from moldy corn contaminated with Fusarium graminearum for the first time by Stob et al. in 1962. Zearalenone is a secondary metabolite mainly synthesized and secreted by Fusarium fungi such as Fusarium graminearum, Fusarium roseum, Fusarium maniliborme and Fusarium tricinctum The product is a reproductive system toxin with a strong teratogenic effect, which can cause serious consequences such as livestock reproductive disorders, abortion and stillbirth, immunosuppression, and decreased growth rate, bringing huge harm to animal husbandry. Because the fungi of the genus Fusarium have strong ecological adaptability and can live both parasitic and saprophytic life, there is a possibility of developing zearalenone contamination from ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23L5/20A23K10/18C12R1/685
CPCC12N1/145C12R2001/685
Inventor 姜旋张素琴罗永祥胡智鹏许加娟黎明张世敏康汇然叶维民贾艳林王跃球魏友岗高遵波梁汉新周桂香潘沼羽朱瑶文少怀李林蒋宇康建南
Owner 湖南微草生物科技有限公司
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