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Alfalfa salt tolerance gene MsCDPK and encoded protein and application thereof

A salt-tolerant gene, alfalfa technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems that are unfavorable to the normal growth and development of crops and restrict the development of agriculture and animal husbandry

Active Publication Date: 2016-08-10
HARBIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Zhaodong alfalfa, belonging to tetraploid alfalfa, is widely planted as the main grass species in Northeast China, but soil salinization in Northeast China is not conducive to the normal growth and development of crops, which seriously restricts the development of agriculture and animal husbandry

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  • Alfalfa salt tolerance gene MsCDPK and encoded protein and application thereof
  • Alfalfa salt tolerance gene MsCDPK and encoded protein and application thereof
  • Alfalfa salt tolerance gene MsCDPK and encoded protein and application thereof

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Embodiment Construction

[0025] A new salt-tolerant gene MsCDPK disclosed by the present invention is obtained from Medicago sativa, and the specific related experiments are as follows:

[0026] 1. Acquisition of MsCDPK gene from Medicago sativa

[0027] Search and download known wheat TaCDPK sequence text files in the NCBI database (http: / / www.ncbi.nlm.nih.gov / ), from the Medicago truncatula Genome Project website (http: / / medicago.jcvi.org / medicago / ) Download the database of Medicago truncatula, apply the ncbi-blast-2.2.30+ software downloaded from the NCBI website to perform Local Blast, and obtain the CDPK gene sequence of Medicago truncatula. Using this sequence to design primers, the primers are:

[0028] MtCDPK-F: 5'-CAGAGGCGATGGGCAATACA-3';

[0029] MtCDPK-R: 5'-GCCACGAGACTAATGAGCAC-3'.

[0030] The specific process is as follows:

[0031] 1) RNA extraction: RNAprep Pure Plant Total RNA Extraction Kit (Tiangen) was used to extract total RNA from young leaves of alfalfa;

[0032] 2) Obtain...

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Abstract

The invention discloses an alfalfa salt tolerance gene MsCDPK and encoded protein and application thereof and belongs to the technical field of plant genetic engineering. The full length of an open code reading box of the MsCDPK gene is 1650 bp, 549 amino acids are coded, the nucleotide sequence is as shown in SEQ. ID. NO.1, the gene is obtained through nucleotide sequence information, a cloning vector and a plant expression vector of the gene are constructed, the gene is transmitted into model plant tobacco through an agrobacterium-mediated method, the transgenic tobacco is cultured on an MS culture medium containing a salt ingredient, the result indicates that the transgenic plant has high salt tolerance through comparison, it is indicated that expression of the MsCDPK gene improves salt resistance of the plant, and it is hinted that the gene can participate in the salt resistance process of plants. The MsCDPK gene provides a theoretical basis for research on the alfalfa salt-resistant molecular mechanism and breeding.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and relates to the acquisition of an alfalfa salt-resistance-related gene MsCDPK, in particular to an alfalfa salt-tolerant gene MsCDPK and its encoded protein and application. Background technique [0002] Alfalfa (Medicago sativa L.), as an important leguminous herbage in my country and even in the world, is famous for its high yield, excellent grass quality, rich nutrition, strong adaptability, and ability to grow well on mildly saline land. are increasingly valued. Zhaodong alfalfa, belonging to tetraploid alfalfa, is widely planted as the main grass species in Northeast China, but soil salinization in Northeast China is not conducive to the normal growth and development of crops, which seriously restricts the development of agriculture and animal husbandry. Therefore, it is of great significance to discover the salt-tolerant genes in alfalfa and further study the salt-resis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N9/12C12N15/82A01H5/00
CPCC12N9/12C12N15/8273C12Y207/11017
Inventor 郭长虹杜秉昊张雪安逸民张军
Owner HARBIN NORMAL UNIVERSITY