Method for fermenting cellulosic ethanol by thick mash semi-simultaneous saccharification by batch material supplementation

A cellulosic ethanol and fed-batch technology, which is applied in the field of biomass resource recycling and biomass energy, can solve problems such as unsatisfactory production efficiency and poor technical comprehensive indicators, and achieve the effect of reducing commercial costs

Active Publication Date: 2016-08-10
JIANGNAN UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that the comprehensive index of the existing cellulose ethanol thick mash fermentation technology is not good, that is, the ethanol concentration obtained by the thick mash fermentation reaches the industrial standard, but the production efficiency is not ideal, and a fed-batch semi-saccharification fermentation is proposed Process for producing cellulosic ethanol

Method used

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  • Method for fermenting cellulosic ethanol by thick mash semi-simultaneous saccharification by batch material supplementation
  • Method for fermenting cellulosic ethanol by thick mash semi-simultaneous saccharification by batch material supplementation
  • Method for fermenting cellulosic ethanol by thick mash semi-simultaneous saccharification by batch material supplementation

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Embodiment 1

[0023] 1) Steam explosion treatment: Put corn stalks into a steam explosion reactor at 200°C for 10 minutes to obtain a solid-liquid separation, and dry the solid part at 60°C for 6 hours; after steam explosion treatment, the three major components are: fiber The content of xylan is 33%, the content of xylan is 3.32%, and the content of lignin is 36.1%.

[0024] 2) Treatment with alkaline hydrogen peroxide solution: then use 2% (w / w) hydrogen peroxide solution at pH 11.5, solid-to-liquid ratio 1:20, and temperature 25°C for 6 hours and 3 times . The obtained solid-liquid mixture is then subjected to solid-liquid separation, and then washed with hot water until neutral. Finally, dry it at 60°C to obtain a dry solid rich in cellulose. The content of the three major components after treatment with alkaline hydrogen peroxide solution is 61.2% of cellulose, 5.21% of hemicellulose and 9.26% of lignin.

[0025] 3) Feed-batch semi-saccharification concentrated mash to ferment ethan...

Embodiment 2

[0027] 1) Steam explosion treatment: Put corn stalks into a steam explosion reactor at 200°C for 10 minutes to obtain a solid-liquid separation, and dry the solid part at 60°C for 6 hours; after steam explosion treatment, the three major components are: fiber The content of xylan is 33%, the content of xylan is 3.32%, and the content of lignin is 36.1%.

[0028] 2) Alkaline hydrogen peroxide solution treatment: then use 4% (w / w) hydrogen peroxide solution at pH 11.5, solid-to-liquid ratio 1:8, temperature 25°C, treatment time 6h, treatment 2 times . The obtained solid-liquid mixture is then subjected to solid-liquid separation, and then washed with hot water until neutral. Finally, dry it at 60°C to obtain a dry solid rich in cellulose. The content of the three major components after treatment with alkaline hydrogen peroxide solution is 62.6% of cellulose, 4.71% of hemicellulose and 8.54% of lignin.

[0029] 3) Fed-batch semi-simultaneous saccharification and fermenting con...

Embodiment 3

[0031] 1) Steam explosion treatment: Put corn stalks into a steam explosion reactor at 200°C for 10 minutes to obtain a solid-liquid separation, and dry the solid part at 60°C for 6 hours; after steam explosion treatment, the three major components are: fiber The content of xylan is 33%, the content of xylan is 3.32%, and the content of lignin is 36.1%.

[0032] 2) Alkaline hydrogen peroxide solution treatment: then use 4% (w / w) hydrogen peroxide solution at pH 11.5, solid-to-liquid ratio 1:8, temperature 25°C, treatment time 6h, treatment 2 times . The obtained solid-liquid mixture is then subjected to solid-liquid separation, and then washed with hot water until neutral. Finally, dry it at 60°C to obtain a dry solid rich in cellulose. The content of the three major components after treatment with alkaline hydrogen peroxide solution is 62.6% of cellulose, 4.71% of hemicellulose and 8.54% of lignin.

[0033]3) Feed-batch semi-saccharification concentrated mash to ferment et...

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Abstract

The invention provides a method for fermenting cellulosic ethanol by thick mash semi-simultaneous saccharification by batch material supplementation. The method comprises the following steps: 1) steam explosion treatment; 2) alkaline hydrogen peroxide treatment: the hydrogen peroxide concentration (w/v) is 0.5-5%, the solid-to-liquid ratio is 1:5-1:20, the pH value is 9-12, the temperature is 10-70 DEG C, and the treatment is carried out 1-5 times for 1-10 hours; and 3) batch-material-supplementation thick mash semi-simultaneous-saccharification ethanol fermentation: respectively sterilizing the obtained solid and nutrient-containing solution at high temperature, mixing the solid and solution with a cellulase subjected to filtration sterilization, oscillating at 45-50 DEG C for 8-16 hours, adding a dry material which is 0.8-2 times of the initial amount and a corresponding amount of enzyme solution into the system, continuing oscillating at 45-50 DEG C for 12-30 hours, and after the enzymolysis finishes, inoculating Saccharomyces cerevisiae to carry out ethanol fermentation. The method provides a production way for cellulosic ethanol commercialization.

Description

technical field [0001] The invention belongs to the technical field of biomass resource recycling and biomass energy, and specifically relates to a method for fermenting cellulosic ethanol through fed-batch semi-synchronous saccharification of concentrated mash Background technique [0002] With the rapid development of modern industry and the continuous increase of the global population, people's demand and consumption of various energy fuels and chemicals are increasing day by day, which has led to increasingly prominent problems such as food crisis, energy crisis, greenhouse effect and environmental pollution . The above problems make people realize the necessity and urgency of large-scale development of clean and renewable energy. At present, many countries have promoted the biomass energy industry as a major national strategy. Among the many biofuels and bio-based chemicals, cellulosic ethanol is recognized as one of the most valuable renewable energy sources due to i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12P19/14C12R1/865
CPCC12P7/10C12P19/14C12P2201/00Y02E50/10
Inventor 孙付保刘建权张震宇洪嘉鹏赵晓琴张世敏
Owner JIANGNAN UNIV
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