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Peroxynitrite ion detection probe, its preparation method and application

A technology for peroxynitrite and ion detection, which is applied to measurement devices, material analysis by optical means, material excitation analysis, etc., can solve problems such as rare reports, and achieves simple operation, simple preparation method, and difficult photobleaching. Effect

Active Publication Date: 2019-04-05
NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the construction of ONOO based on inorganic fluorescent nanocrystals - Reports of probes are rare

Method used

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  • Peroxynitrite ion detection probe, its preparation method and application
  • Peroxynitrite ion detection probe, its preparation method and application
  • Peroxynitrite ion detection probe, its preparation method and application

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preparation example Construction

[0035] The embodiment of the present invention also provides a kind of preparation method of peroxynitrite ion detection probe, comprising:

[0036] using a coupling reagent to react with the fluorescent nanomaterial to form a first active group on the surface of the fluorescent nanomaterial,

[0037] making the second active group in the coenzyme react with the first active group distributed on the surface of the fluorescent nanomaterial, so that the coenzyme and the surface of the inorganic fluorescent material are chemically bonded to form the peroxysuboxide Nitrate ion detection probe.

[0038] Further, the first reactive group includes a carboxyl group, and the second reactive group includes an amino group.

[0039] Wherein, the amount of the fluorescent nanomaterial can be 0 to 20 parts by weight (preferably greater than 5 and less than or equal to 20 parts by weight), and the amount of the enzyme can be 5 to 100 parts by weight (preferably greater than 0 and less than ...

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Abstract

The invention discloses a peroxynitrite ion detection probe as well as a preparation method and application thereof. The peroxynitrite ion detection probe comprises a fluorescent nano material and an enzyme, wherein the enzyme is combined with the fluorescent nano material, has an oxidation-reduction property and can be selectively oxidized by peroxynitrite ions to form an oxidized enzyme; after the fluorescent nano material is radiated by excited light, electrons in an excited state can be transferred to the oxidized enzyme and cannot turn back to a base state, and then fluorescent light of the fluorescent nano material can be quenched. As an inorganic light-emitting material is adopted as fluorophore, the peroxynitrite ion detection probe disclosed by the invention is intense in light emission, good in stability and less prone to photo-bleach; the surface of the probe is modified with the enzyme with oxidation-reduction property, and the enzyme can be selectively oxidized by ONOO<->, so that the probe is not interfered by other active oxygen (ROS) or active nitrogen (RNS), and is excellent in specificity.

Description

technical field [0001] The present invention specifically relates to a kind of peroxynitrite ion (ONOO - ) detection probe, its preparation method and application, such as the application in the selective determination of peroxynitrite ion in detection biological system. Background technique [0002] Peroxynitrite ion (ONOO - ) is a strong oxidizing substance produced by the reaction of nitric oxide and superoxide anion in the body. It may be an important link in the pathological damage of nitric oxide. At present, ONOO has been found in shock, ischemia-reperfusion injury, atherosclerosis, Alzheimer's disease and other diseases - The presence. Therefore, developed for ONOO - A highly sensitive, highly selective fluorescent probe for ONOO - The detection analysis and imaging will be of great significance to physiological research. [0003] Currently, the vast majority of detection ONOO - The fluorescent probes are designed based on organic dyes, by covalently coupling...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6432
Inventor 王宇辉林恒伟朱佳丽
Owner NINGBO INST OF MATERIALS TECH & ENG CHINESE ACADEMY OF SCI
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