Method for rapidly detecting content of basic group in food

A fast and basic technology, applied in the field of chemical analysis, it can solve the problems of residual liquid loss, equipment occupying working space, improper sample dilution, etc., to reduce analysis errors and save space.

Inactive Publication Date: 2016-08-24
广东江门生物技术开发中心有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These conventional utensils have single functions. When dealing with a large number of samples or complicated steps, a large number of utensils need to be used and cleaned after use, the utensils occupy the working space, the operation is cumbersome, and residual liquid loss or improper sample dilution occurs during the transfer process. and many other problems, resulting in defects or deficiencies such as high intensity of detection work, low efficiency, and large detection errors.

Method used

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  • Method for rapidly detecting content of basic group in food
  • Method for rapidly detecting content of basic group in food
  • Method for rapidly detecting content of basic group in food

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A method for quickly detecting the base content in edible yeast powder, using the multifunctional test sample extraction and separation device provided by the present invention, specifically includes the following steps:

[0042] (a) Digestion reaction: Add 5ml of 1% edible yeast powder aqueous solution and 5ml of perchloric acid in sequence in the reaction device, mix well, fill with nitrogen, seal with a sealing plate, and tighten the first quick clamp, Heating in a water bath at 100°C for 60 minutes;

[0043] (b) Extraction: Open the first quick clamp and sealing plate, pour the reaction solution in the reaction device into the extraction device, at this time, the bottom of the extraction device is a sealed structure; wash the reaction device twice with a small amount of pure water, And transfer the washing liquid to the extraction device, under stirring, adjust the pH value of the reaction liquid to 7.0 with 10mol / L potassium hydroxide solution, and let it stand for...

Embodiment 2

[0052] A method for rapidly detecting the base content in yeast extract, using the multi-functional test sample extraction and separation device provided by the present invention, specifically includes the following steps:

[0053] (a) Digestion reaction: Add 5ml of 5% yeast extract aqueous solution and 10ml of perchloric acid in sequence in the reaction device, mix well, fill with nitrogen, seal with a sealing plate, and tighten the first quick clamp , heating and reacting at 120°C for 20 minutes;

[0054] (b) Extraction: Open the first quick clamp and sealing plate, pour the reaction solution in the reaction device into the extraction device, at this time, the bottom of the extraction device is a sealed structure; wash the reaction device twice with a small amount of pure water, And transfer the washing solution to the extraction device, under stirring, adjust the pH value of the reaction solution to 7.0 with 1mol / L sodium hydroxide solution, and let it stand for 10 minutes;...

Embodiment 3

[0063] A method for quickly detecting the base content in broken-walled edible yeast, using the multifunctional test sample extraction and separation device provided by the present invention, specifically includes the following steps:

[0064] (a) Digestion reaction: Add 5ml of broken wall edible yeast with a mass fraction of 5% and 10ml of perchloric acid in sequence in the reaction device, mix well, fill with nitrogen, seal with a sealing plate, and tighten the first quick clamp , heating and reacting in a water bath at 100°C for 30 minutes;

[0065] (b) Extraction: Open the first quick clamp and sealing plate, pour the reaction solution in the reaction device into the extraction device, at this time, the bottom of the extraction device is a sealed structure; wash the reaction device twice with a small amount of pure water, And the washing liquid is transferred to the extraction device, under the stirring state, adjust the pH value of the reaction liquid to 7.0 with 20mol / L ...

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Abstract

The invention provides a method for rapidly detecting the content of a basic group in food. An integrated device is adopted, and all processes such as digestion and degradation, interfering substance precipitation, target constituent isolation and detection and analysis can be completed. Compared with the existing multi-device and multi-link treatment technologies, the method for rapidly detecting the content of the basic group in the food is obviously improved with respect to detection precision, operational convenience, detection efficiency and the like and has the advantages that work intensity is alleviated, and the space occupied by devices is saved; when the method is used for detecting a great number of detection articles, higher rapidness and convenience are achieved. By means of the rapid and accurate detection method, on one hand, enterprises and consumers can know about information of the purine content in the food, the situation that diseases such as gout and hyperuricemia are induced due to excessive intake of the human body is avoided, and the national health level is raised; on the other hand, nucleotide and basic group type products serve as important raw materials of food and pharmaceutical industries, as a result of improvement of the detection technology, the food manufacturing level and food safety guarantee level can be pushed to be raised, and it is helpful to promote economic development and social harmony at the same time.

Description

technical field [0001] The invention relates to the technical field of chemical analysis, in particular to a method for quickly detecting the content of bases (various purines and pyrimidines) in food, which is suitable for operations such as sample digestion, extraction, separation, and detection in the base content analysis process. Background technique [0002] Nucleic acids, nucleotides, and decomposed bases are rich in purines. Excessive intake by humans will induce diseases such as gout and hyperuricemia, and seriously affect the health of human kidneys, limbs, and joints. However, at present, with the development and popularization of fresh-enhancing technology, some food companies randomly add guanylic acid and inosinic acid (I+G), yeast extract, seafood extract, etc. to food in order to improve the taste and umami taste. High purine substances, and are not marked in detail on food labels. Therefore, providing a rapid and accurate technology for detecting purine con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/14
CPCG01N30/02G01N30/14G01N2030/027
Inventor 李富雄
Owner 广东江门生物技术开发中心有限公司
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