Method for rapidly detecting content of basic group in food
A fast and basic technology, applied in the field of chemical analysis, it can solve the problems of residual liquid loss, equipment occupying working space, improper sample dilution, etc., to reduce analysis errors and save space.
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Embodiment 1
[0041] A method for quickly detecting the base content in edible yeast powder, using the multifunctional test sample extraction and separation device provided by the present invention, specifically includes the following steps:
[0042] (a) Digestion reaction: Add 5ml of 1% edible yeast powder aqueous solution and 5ml of perchloric acid in sequence in the reaction device, mix well, fill with nitrogen, seal with a sealing plate, and tighten the first quick clamp, Heating in a water bath at 100°C for 60 minutes;
[0043] (b) Extraction: Open the first quick clamp and sealing plate, pour the reaction solution in the reaction device into the extraction device, at this time, the bottom of the extraction device is a sealed structure; wash the reaction device twice with a small amount of pure water, And transfer the washing liquid to the extraction device, under stirring, adjust the pH value of the reaction liquid to 7.0 with 10mol / L potassium hydroxide solution, and let it stand for...
Embodiment 2
[0052] A method for rapidly detecting the base content in yeast extract, using the multi-functional test sample extraction and separation device provided by the present invention, specifically includes the following steps:
[0053] (a) Digestion reaction: Add 5ml of 5% yeast extract aqueous solution and 10ml of perchloric acid in sequence in the reaction device, mix well, fill with nitrogen, seal with a sealing plate, and tighten the first quick clamp , heating and reacting at 120°C for 20 minutes;
[0054] (b) Extraction: Open the first quick clamp and sealing plate, pour the reaction solution in the reaction device into the extraction device, at this time, the bottom of the extraction device is a sealed structure; wash the reaction device twice with a small amount of pure water, And transfer the washing solution to the extraction device, under stirring, adjust the pH value of the reaction solution to 7.0 with 1mol / L sodium hydroxide solution, and let it stand for 10 minutes;...
Embodiment 3
[0063] A method for quickly detecting the base content in broken-walled edible yeast, using the multifunctional test sample extraction and separation device provided by the present invention, specifically includes the following steps:
[0064] (a) Digestion reaction: Add 5ml of broken wall edible yeast with a mass fraction of 5% and 10ml of perchloric acid in sequence in the reaction device, mix well, fill with nitrogen, seal with a sealing plate, and tighten the first quick clamp , heating and reacting in a water bath at 100°C for 30 minutes;
[0065] (b) Extraction: Open the first quick clamp and sealing plate, pour the reaction solution in the reaction device into the extraction device, at this time, the bottom of the extraction device is a sealed structure; wash the reaction device twice with a small amount of pure water, And the washing liquid is transferred to the extraction device, under the stirring state, adjust the pH value of the reaction liquid to 7.0 with 20mol / L ...
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