Preparation method of a diagnosis and treatment preparation integrating ultrasound contrast-enhanced imaging and hyperthermia

Abstract

The invention relates to a preparation method of a diagnosis and treatment preparation integrating ultrasound contrast and thermal therapy. The preparation method comprises the following steps: (1) dissolving 1-50 mg of organic-inorganic complex lipid and 0.1-10 mg of polyethylene glycol into 0.1-1 mL of absolute ethyl alcohol; (2) dissolving 1-25 mg of menthol into 1-20 mL of ultrapure water, carrying out water-bath heating till the temperature reaches 45 DEG C, mixing the obtained solution with 1-10 mL of an aqueous solution of indocyanine green; (3) rapidly injecting the ethanol solution of the organic-inorganic complex lipid and polyethylene glycol obtained in the step (1) into the mixed solution obtained in the step (2) under the water bath and ultrasonic wave conditions; and (4) carrying out probe ultrasound treatment, thus obtaining organic-inorganic complex lipid nano-particles, wherein the inner cavities of the organic-inorganic complex lipid nano-particles are loaded with the indocyanine green and menthol and the surfaces of the organic-inorganic complex lipid nano-particles are covered by inorganic silicate shells being in nanoscale thickness and having good biocompatibility. The complex lipid diagnosis and treatment preparation provided by the invention can realize the photothermal therapy on tumor tissues under the ultrasound visual observation, the stability is good, no obvious toxic and side effects are caused to the human body, in addition, the preparation method is simple, and the requirements on devices are low.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a method for preparing a diagnosis and treatment preparation integrating ultrasound contrast-enhanced imaging and hyperthermia. Background technique [0002] Cancer is a highly heterogeneous and highly adaptable disease, and it is necessary to choose different types of diagnosis and treatment options according to the characteristics of the patient and the progress of the disease. A class of nano-therapeutic preparations that integrate diagnostic and therapeutic functions has gradually emerged in the field of tumor treatment. The combination of nano-preparations in diagnosis and treatment can provide patients with a variety of personalized diagnosis and treatment options, thereby improving the treatment effect. Therefore, it is of great significance to develop new multifunctional nano-diagnosis and treatment preparations. Ultrasound imaging is a widely used diagnostic method i...

AI Technical Summary

Problems solved by technology

Although micron-scale ultrasound contrast agents represented by SonoVue have been successfully applied to varying degrees, there are still many problems: on the one hand, the particle size is large, so it is only limited to the development of the blood pool, and it is not easy to realize the contrast agent outside the blood vessel. Diagnosis and treatment of diseases; on the other hand, because the capsule material of Sonovo is lipid, the lipid properties are not stable enough, the duration in the body is not long enough, and the duration of contrast is short, so it is necessary to use microbubble contrast agent to achieve tumor detection. Molecular imaging still has certain difficulties

However, free indocyanine green is easy to aggregate rapidly in polar solvents, and its stability is poor. It is easy to be quickly cleared in plasma and has a short circulation time in the body. These defects greatly limit the application of indocyanine green.

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