Micro-fluidic chip for high-throughput screening of blood brain barrier drug permeability and preparation method of micro-fluidic chip

A microfluidic chip and blood-brain barrier technology, applied in the field of biomedical detection and analysis, can solve the problems of difficult model physiological activity monitoring, complex detection operation, difficult quantitative analysis, etc., and achieve high-throughput drug permeability Effects of screening, low cost, and consumption reduction
CN105925480AInactive Publication Date: 2016-09-07DALIAN UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
DALIAN UNIV OF TECH
Publication Date
2016-09-07
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention relates to a micro-fluidic chip, which is applicable to researches on blood brain barrier drug permeability and high-throughput screening. The chip is prepared from elastic polydimethylsiloxane by virtue of a molding method. The chip, which is of a three-layer structure, comprises an array of nine groups of parallel micro-reaction units, wherein each of the micro-reaction units is composed of an upper cell culture chamber layer, a lower cell culture chamber layer and a microporous membrane layer, and each of the micro-reaction units comprises a chip micro-electrode and a fluid circulating pipeline. By continuously injecting a culture solution to the cell culture chambers, a fluid state in blood vessels can be simulated, so that a fluid environment is closer to a real internal environment of human body. By virtue of the chip micro-electrodes, electrical impedance signals are collected, and the physiological activity situations of the cell layers in the chip are monitored in real time. Drug molecules, which penetrate through the microporous membranes and the cell layers, deposit in the fluid circulating pipeline on the lower part of the chip and are eluted by virtue of the pipeline, and then membrane-permeable drugs can be collected for subsequent analysis. The micro-fluidic chip disclosed by the invention, compared with an existing method, can reflect blood brain barrier permeability of drugs, and the micro-fluidic chip can conduct high-throughput screening on nine drugs in a targeted mode in one time.
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Description

technical field

[0001] The invention belongs to the field of biomedical detection and analysis, in particular to a microfluidic chip used for high-throughput screening of blood-brain barrier drug permeability. Background technique

[0002] The Blood Brain Barrier (BBB) ​​is a layer between blood and brain tissue that is selective for the permeability of substances. It is composed of endothelial cells, continuous basement membrane and astrocyte foot plate. Intermittent film formation. Normally, the blood-brain barrier acts as a hindrance to substances, allowing only gas molecules and fat-soluble molecules with relatively small molecular weights to pass through, which is beneficial to protecting brain tissue from damage and stabilizing the internal environment of the nervous system. However, this feature also makes it difficult for more than 95% of drugs to penetrate the blood brain and enter the central nervous system, making it difficult to exert drug effects, which greatly...

Claims

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