Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of pcr reaction system for scot molecular marker of berry

A molecular marker and reaction system technology, applied in the biological field, to achieve the effects of good repeatability, high sensitivity and simple operation

Inactive Publication Date: 2019-08-30
TIBET AGRI & ANIMAL HUSBANDRY COLLEGE
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report about the establishment of the SCoT-PCR reaction system of fenugreek

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of pcr reaction system for scot molecular marker of berry
  • A kind of pcr reaction system for scot molecular marker of berry
  • A kind of pcr reaction system for scot molecular marker of berry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The method for establishing the PCR reaction system of the SCoT molecular marker of embodiment 1

[0020] 1 Experimental method

[0021] 1.1 Genomic DNA extraction and detection

[0022] The extraction of plant genomic DNA of berry plant adopts the improved CTAB method (Li Jinlu, Wang Shuo, Yu Jing, Wang Ling, & Zhou Shiliang. (2013). An improved plant DNA extraction method. Acta Botany, 48(1), 72-78.). The DNA concentration and quality were determined by a spectrophotometer, and an appropriate amount of samples were taken for detection by 0.8% agarose electrophoresis, and the rest of the DNA samples were stored in a -20°C refrigerator for later use.

[0023] 1.2 Optimization of SCoT-PCR reaction system

[0024] The reaction system was determined to be 20 μL, and the pre-amplified primer SC2 (5'-CAACAATGGCTACCACCC-3', whose nucleotide sequence is shown in SEQ ID NO.1) was first used as the Adenophyllum genomic DNA amplification system to establish Primary primers, T...

Embodiment 2

[0039] The application example of the PCR reaction system of the SCoT molecular marker of embodiment 2

[0040] On the basis of the optimal SCoT-PCR reaction system, 8 randomly selected samples of A. adenocarpa were amplified using SC35 (5'-CATGGCTACCACCGGCCC-3', whose nucleotide sequence is shown in SEQ ID NO.2) primers .

[0041] By amplifying 8 randomly selected berry samples, the results ( image 3 ) showed that the 8 samples were effectively amplified, and the amplified bands were clear, that is, the established SCoT-PCR reaction system of A. adenifolia was stable.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a PCR (Polymerase Chain Reaction) system of a SCoT (Start Codon Targeted Polymorphism) molecular marker for rubus biflorus buch. The PCR system is prepared from the following components with the following content: 0.125mmol.L<-1> of dNTPs (Deoxynucleotide Triphosphates), 0.75U of Taq DNA (Deoxyribonucleic Acid) polymerase, 1.0mu mol.L<-1> of primer, 0.625mmol.L<-1> of Mg<2+>, 40ng of template and the balance of water; an annealing temperature is 51 DEG C; the total amount of the above components is 20.0mu L. the invention also discloses a PCR procedure of the SCoT molecular marker for the rubus biflorus buch. A strip amplified by a SCoT-PCR system for the rubus biflorus buch, which is established by the invention, is high in stability and high in definition, and has the characteristic of quite high polymorphism. The deficiency of the domestic research on the genetic diversity of the rubus biflorus buch is made up.

Description

technical field [0001] The application belongs to the field of biotechnology, and in particular relates to a PCR reaction system for SCoT molecular markers of fenugreek. Background technique [0002] Rubus biflorus Buch is a deciduous wild fruit tree in the genus Rubus of the family Rosaceae, and its distribution in Tibet is mainly concentrated in Lhasa, Nyingchi and other places. Its fruit is sweet, aggregated berries, orange red, rich in nutrients, organic acids, iron and zinc and other trace elements are higher than ordinary fruits, protein and vitamin content are also much higher than ordinary fruits, in addition to being used for raw food, also It can make wine, make fruit juice, jam, etc., and has high development and utilization value. At present, the researches on Berry japonica mainly focus on resource investigation, analysis of nutritional components and active components, but there is no report on the genetic diversity of Berry berry. Therefore, carrying out the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/10
Inventor 袁雷钟政昌刘瑜张立谢博曹东星
Owner TIBET AGRI & ANIMAL HUSBANDRY COLLEGE