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Preparation method of Echinodorus amazonicus artificial seed

A technology of artificial seeds and crown, which is applied in the field of preparation of artificial seeds of Phaleria cuspidatum, can solve the problems of high environmental requirements and low reproduction rate, and achieve the effects of low cost, high germination rate and shortened reproduction time

Inactive Publication Date: 2016-09-21
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the reproduction rate of this breeding method is too low, and the requirements for the environment are high, and the existing breeding methods cannot meet the actual needs.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0016] Select the young leaves scattered on the stolons of Crown Grass, and use tweezers to break off them for later use.

[0017] (1) After rinsing the young blades of Phaleria macrocarpa with tap water, soak the blades in 70% alcohol for 40 seconds on the ultra-clean workbench, wash them twice with sterile water, then disinfect the surface with 3% sodium hypochlorite for 5 minutes, and use sterile Rinse with distilled water 4 times.

[0018] (2) Blade cut into 5mm 2 About, inoculated on MS medium containing 0.8 mg / L BA (6-benzylaminopurine), 1.1 mg / LZT (zeatin), 3% sucrose and 0.8% agar. The back side of the leaf faces downward, the culture temperature is 25°C, the light cycle is 16h:8h, and the culture is 30 days.

[0019] (3) After culturing for 30 days, the somatic embryos were peeled off on an ultra-clean workbench, and placed in an embedding solution containing 3% sodium alginate and 1 / 5MS+1 mg / L indole acetic acid (IAA) with tweezers, Mix somatic embryos and embeddi...

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PUM

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Abstract

The invention provides a preparation method of an Echinodorus amazonicus artificial seed. The method consists of: taking Echinodorus amazonicus tender leaf as the explant, cultivating the explant on an MS medium attached with BA (6-benzylaminopurine) and ZT (zeatin) for 30d to obtain a mature somatic embryo of Echinodorus amazonicus, cutting off the somatic embryo under an aseptic condition, and adding the cut somatic embryo into a 3% sodium alginate solution containing the MS medium and conducting mixing, dripping an embedding solution containing the somatic embryo into a 2% calcium chloride solution by a sterile dropper, half an hour later, performing rinsing with sterile water, thus obtaining the Echinodorus amazonicus artificial seed. The method provided by the invention solves the problem of low reproduction rate of Echinodorus amazonicus during cultivation, takes the easily available leaves as the explant material, and is not affected by seasons, the propagation speed is fast, and the survival rate is high.

Description

technical field [0001] The invention belongs to the technical field of plant artificial seed production, and in particular relates to a method for preparing an artificial seed of Phaleria macrocarpa. Background technique [0002] Crown grass, also known as Amazon sword grass, is native to South America. It is a large perennial submerged aquatic plant. It is often used for aquarium landscaping because of its wide leaves and beautiful leaf shape. Crown grass is mainly propagated by ramets, and the breeding water temperature should be kept at 20-26°C. When the scattered shoots on the stolon grow to 6-8cm, they will be broken off with roots and planted separately. However, the reproduction rate of this breeding method is too low, and the requirements for the environment are relatively high, and the existing breeding methods cannot meet the actual needs. [0003] Artificial seeds refer to the granules formed by embedding somatic embryos or meristematic tissues that can develop ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 李映霞
Owner QINGDAO AGRI UNIV