Hybrid snakehead spermatozoa preserving fluid and application thereof

A technology for preserving liquid and sperm, applied in the field of fish farming, can solve the problems of sperm inactivation and asynchrony of gonad development, and achieve the effects of long storage life, good sperm motility, and easy operation.

Active Publication Date: 2016-09-21
渔仁堂生物科技(湛江)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The preservation solution provided by the present invention can better solve the problem of asynchronous gonad development and the proble

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Accurately weigh 8.2 g of sodium chloride, 0.47 g of potassium chloride, 0.128 g of calcium chloride, 0.4 g of sodium bicarbonate, and 10 g of glucose, dissolve them in 1 liter of distilled water, and prepare a sperm preservation solution (total osmotic pressure of 343 mOsm / kg, pH 7.4).

Embodiment 2

[0019] Accurately weigh 8.5g of sodium chloride, 0.5g of potassium chloride, 0.15g of calcium chloride, 0.6g of sodium bicarbonate, and 14g of glucose, dissolve them in 1 liter of distilled water, and prepare a sperm preservation solution (the total osmotic pressure is 380mOsm / kg , adjust the pH to 7.4).

Embodiment 3

[0026] Example 3: Sperm motility assay

[0027] The hybrid snakehead spermatozoa and preservation solution were diluted at a ratio of 1:9, and stored at 4°C. After the sperm were preserved in the preservation solution at 4°C for 96 hours, their viability and movement time were measured.

[0028] Determination of sperm motility in Examples and Comparative Examples: 0.5 ml of diluted semen was added dropwise on a common glass slide, 0.5 ml of water was added to activate, and the motility was immediately observed under a 10×16 magnification microscope. The results are shown in Table 1:

[0029] Table 1

[0030] Fast motion time (s) Total life (s) Activation rate (%) Example 1 177.4 206.3 94.7 Example 2 152.7 189.4 90.4 Comparative example 1 127.3 140.2 83.7 Comparative example 2 135.6 167.8 85.9 Comparative example 3 110.8 130.5 81.4

[0031] As can be seen from the results in Table 1, the sperm preservation solut...

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PUM

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Abstract

The invention provides a hybrid snakehead spermatozoa preserving fluid. Water is taken as solvent, and every one liter of preserving fluid comprises 7.5-8.5g of sodium chloride, 0.4-0.5g of potassium chloride, 0.1-0.15g of calcium chloride, 0.2-0.6g of sodium bicarbonate, 8-15g of glucose and the balance being water. The hybrid snakehead spermatozoa preserving fluid is simple in source, easy to operate and feasible, preservation is carried out at conventional low temperature, spermatozoa preserved with the preserving fluid are long in preservation life, high in activation rate and good in sperm motility, guarantee is provided for spermatozoa preservation and reproduction of hybrid snakehead, and the hybrid snakehead spermatozoa preserving fluid has good application prospect.

Description

[0001] technical field [0002] The invention belongs to the technical field of fish farming, and more specifically relates to a hybrid snakehead sperm preservation solution and an application thereof. Background technique [0003] The hybrid snakehead belongs to Perciformes, Channa suborder, Channaidae and Channa genus in taxonomy. It is commonly known as raw fish in Guangdong and is a ferocious freshwater carnivorous fish. The hybrid snakehead is delicious and nutritious, and is widely welcomed in the market. In recent years, the main species cultivated in the market is the hybrid snakehead produced by crossing the snakehead (♂) with the same species of snakehead (♀). Both snakeheads and snakeheads are spawning fish in batches. During the breeding process of hybrid snakeheads, they often face the phenomenon that the sexual maturity of female and male fish is not synchronized or the female maturity is insufficient. In order to avoid the waste of sperm, cryopreservation of...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 汤保贵何飞飞陈刚张健东
Owner 渔仁堂生物科技(湛江)有限公司
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