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Culture medium enhancer suitable for isolating culture of brucella

A Brucella culture medium technology, applied in the field of medium enhancer, can solve the problem of not supporting the growth of Brucella species and the effect of biochemical tests, hindering the popularization and application of Brucella hygiene experiments, Affect the growth of Brucella, etc., to achieve the effect of being suitable for large-scale production and use, inhibiting the interference of miscellaneous bacteria, and being convenient to prepare and use

Inactive Publication Date: 2016-09-28
连云港市产品质量监督检验中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many brucella culture mediums at home and abroad generally have a single component or use potato components, which significantly affect the growth of different species of brucella. Supports the expression of growth and biochemical assay effects for all Brucella species, thereby hindering the popularization and application of Brucella hygiene assays

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1 Detection of mutton samples by isolation and culture of Brucella. It is used as follows:

[0070] A kind of substratum enhancer that is suitable for Brucella isolation and cultivation, the content that one liter of double distilled water forms is:

[0071] Erythritol 0.5g

[0072] Interleukin 2 1 μg

[0073] Trypsin 20g

[0074] Glucose 1g

[0075] Sodium chloride 5g

[0076] Horse Serum 50ml

[0077] Niacin 0.5 mg

[0078] Biotin 0.3 mg

[0079] Vitamin B 1 1mg

[0080] D-Cycloserine 321.5mg

[0081] Methyl ether 1:20000

[0082] Polymyxin B 1:4000

[0083] Bacitracin 1:25000

[0084] Cycloheximide 1:100000

[0085] Nystatin 10000 units

[0086] Zinc sulfate 2μg

[0087] Manganese sulfate 10μg

[0088] Copper chloride 1μg

[0089] Cobalt sulfate 2μg

[0090] Boric acid 0.5μg

[0091] Ferric chloride 27μg

[0092] Aluminum trichloride 10μg

[0093] Agar 1.5%

[0094] pH 6.8-7.0

[0095] The above components were fully dissolved in distil...

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Abstract

A culture medium enhancer suitable for isolating culture of brucella is prepared from erythritol, glucose, animal serum, vitamins, amino acid, cell growth factors and the like. The content of ingredients in one liter of distilled water is present in the description, wherein the ingredients includes glucose, erythritol, tryptose, sodium chloride, horse serum, niacin, vitamins, vitamin B1, D-cycloserine, methylhexyl ether, polymyxin B, bacitracin, cycloheximide, nystatin, zinc sulfate, manganese sulfate, copper chloride, cobaltous sulfate, boric acid, ferric trichloride and aluminum trichloride. By using the culture medium enhancer suitable for isolating culture of brucella, growth of brucella in the culture medium can be effectively promoted while the growth of interference bacteria is suppressed. The culture medium enhancer is high in university oriented to the isolating culture of brucella, low in cost and convenient to prepare and use.

Description

technical field [0001] The invention relates to a mycelium culture technology, in particular to a culture medium enhancer suitable for the isolation and culture of Brucella. Background technique [0002] The harm and pollution of Brucella is a kind of biological factors that are more harmful in zoonotic diseases. The analysis and detection of Brucella has not been paid attention to, but both clinical and scientific research need a medium suitable for the cultivation of the bacteria. The currently used medium is selective and sensitive to some species of Brucella during the test The unsatisfactory growth has led to serious missed detection, which is not conducive to the development of scientific research work and food safety work. [0003] As we all know, the effect, economy and efficiency of bacterial culture are determined by the medium, and the optimization of the medium is the core link in the establishment of an efficient bacterial culture test process. Therefore, acco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 陈梦陈建郭瑛周翔周文静杨维琴
Owner 连云港市产品质量监督检验中心
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