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Method for determining naphthol yellow S and rhodamine B in juice and beverages through LC-MS/MS (Liquid Chromatography-Tandem Mass Spectrometry)

A technology of naphthol yellow and beverages, which is applied in the field of determination of artificially synthesized colorants, can solve the problems of lack of confirmation technology, no pretreatment and purification means, and inability to meet the requirements of qualitative confirmation of the target object, and achieve good extraction effect, high sensitivity, The effect of high accuracy

Inactive Publication Date: 2016-09-28
JINAN CUSTOMS TECH CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Liquid chromatography is the most widely used method for the detection of artificially synthesized colorants in food, but it lacks supporting confirmatory techniques and cannot meet the qualitative confirmation requirements of targets in complex food matrices
The reported LC-MS / MS method for the detection of synthetic colorants in food only involves some colorants and there is no universal pretreatment and purification method

Method used

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  • Method for determining naphthol yellow S and rhodamine B in juice and beverages through LC-MS/MS (Liquid Chromatography-Tandem Mass Spectrometry)
  • Method for determining naphthol yellow S and rhodamine B in juice and beverages through LC-MS/MS (Liquid Chromatography-Tandem Mass Spectrometry)
  • Method for determining naphthol yellow S and rhodamine B in juice and beverages through LC-MS/MS (Liquid Chromatography-Tandem Mass Spectrometry)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Determination of naphthol yellow S and rhodamine B in fruit and vegetable juice samples.

[0104] 3 Sample extraction and purification

[0105] 3.1 Extraction

[0106] Take 10g of the shaken sample, put it in a 50mL centrifuge tube, add 25mL of ultrapure water, ultrasonically extract it in a water bath at 60°C for 15min, centrifuge it in a centrifuge at 4000r / min for 10min, pipette the supernatant and adjust the pH value to 3-6, to be purified solution;

[0107] 3.2 Purification

[0108] 3.2.1 Assembly of solid phase extraction column: Weigh 60mg 40-60μm PCX cation exchange packing and 60mg 40-60μm PWAX mixed weak anion exchange packing respectively, fill them in the polypropylene small column with polyethylene sieve plate at the bottom, The upper end of the filler is fixed with a polyethylene sieve plate;

[0109] 3.2.2 Column activation: activate the above-mentioned solid phase extraction cartridge with 6mL of water with a pH value of 6-7 and 6mL of methanol in se...

Embodiment 2

[0115] Determination of naphthol yellow S and rhodamine B in carbonated beverage samples.

[0116] 3 Sample extraction and purification

[0117] 3.1 Extraction

[0118] Take a 500mL sample and ultrasonically remove carbon dioxide in a beaker, seal the label, and set aside;

[0119] Take 10g of the sample, add 10mL of ultrapure water to a 25mL volumetric flask, adjust the pH value to 4 with citric acid aqueous solution, and dilute the volume of ultrapure water to obtain the solution to be purified;

[0120] 3.2 Purification

[0121] 3.2.1 Assembly of solid-phase extraction cartridges: weigh 60mg of 40-60μm PCX cation exchange filler and 60mg of 40-60μm PWAX mixed weak anion-exchange filler, respectively, and fill them in polypropylene cartridges with polyethylene sieve plates at the bottom Inside, the upper end of the filler is fixed with a polyethylene sieve plate;

[0122] 3.2.2 Column activation: activate the above-mentioned solid phase extraction cartridge with 6mL of w...

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Abstract

The invention discloses a method for determining naphthol yellow S and rhodamine B in juice and beverages through LC-MS / MS (Liquid Chromatography-Tandem Mass Spectrometry). The method comprises the following steps: firstly, removing CO2 by ultrasounds, heating and extracting in a water bath, centrifuging and adjusting acid of supernatant; then, performing purification treatment on a sample extracting solution by utilizing a self-assembled solid-phase extraction and purification small column (cation exchange packing+anion exchange packing) so as to effectively remove interference matters and realize purification and enrichment of the naphthol yellow S and the rhodamine B at the same time; quantitatively determining by adopting the LC-MS / MS. The method is simple to operate, good in purification effect, high sensitivity and good in repeatability, meets analysis standards of food additives and has certain novelty and applicability.

Description

technical field [0001] The invention relates to a method for determining artificially synthesized colorants in fruit juices and beverages, in particular to a solid-phase extraction / LC-MS / MS method for naphthol yellow S and rhodamine B in fruit juices and beverages. Background technique [0002] Synthetic colorants are organic pigments produced by chemical synthesis, mainly using aromatic chemical products such as benzene, toluene, and naphthalene as raw materials, and compounded through a series of organic reactions such as sulfonation, halogenation, and azolation. It is low in cost, bright in color, strong in tinting strength and stable in performance, and is widely used in food production and processing industries. However, most of the synthetic colorants are azo compounds, and excessive consumption can cause teratogenic and carcinogenic effects on the human body. In recent years, in order to strengthen the management of food synthetic coloring agents, many countries have...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/027
Inventor 郑新华王乐何桂华韩焕美
Owner JINAN CUSTOMS TECH CENT
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