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Immune lateral chromatographic detection system as well as preparation method and application thereof

A technology of immune lateral chromatography and detection system, which is applied in the field of immune lateral chromatography detection system and its preparation, can solve the problems that the sensitivity, precision and accuracy of the detection and detection system cannot meet the existing needs, and achieve the extension of Effects of distance, reagent consumption reduction, and sensitivity improvement

Inactive Publication Date: 2016-09-28
北京康思润业生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, with the development of society, the sensitivity, precision and accuracy of the current detection and detection system can no longer meet the existing needs.

Method used

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  • Immune lateral chromatographic detection system as well as preparation method and application thereof
  • Immune lateral chromatographic detection system as well as preparation method and application thereof
  • Immune lateral chromatographic detection system as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 (no marker pad, marker in test tube, with sample diluent)

[0035] Coating antibody: Dilute human heart fatty acid binding protein (HFABP) detection antibody 1 with coating buffer to a fixed concentration (2.0mg / ml), and control reagent 1 (goat anti-chicken IgY) to a fixed concentration (2.0mg / ml ), using BioDot’s XYZ3060 to coat the above two liquids on Sartorius nitrocellulose membrane 140 (NC membrane), wherein the quality control zone is located between the detection zone and the sample hole, and dried in a 37°C oven for 4 hours ,spare. Coating buffer is 0.01Mol / l phosphate buffer solution (PBs) plus 3% sucrose as protective agent.

[0036] Labeled antibodies: human heart fatty acid binding protein (HFABP) detection antibody 2 and control reagent 2 (chicken IgY) were fluorescently labeled with latex, stored in storage solution, and ready for use (50mMol / l Tris, 0.5%BSA, pH 7.8).

[0037] Marker preparation: Spray 5 microliters of the above-mentioned labe...

Embodiment 2

[0046] Example 2 (no marker pad, marker in tip, with sample diluent)

[0047] Coating antibody: Dilute C-reactive protein (CRP) detection antibody 1 with coating buffer to a fixed concentration (0.5mg / ml), and control reagent 1 (goat anti-chicken IgY) to a fixed concentration (2.0mg / ml), The above two liquids were coated on the Sartorius nitrocellulose membrane (NC) using XYZ3060 from BioDot Company, where the quality control zone was located between the detection zone and the sample hole, and dried in a 37°C oven for 4 hours before use. Coating buffer is 0.01Mol / l phosphate buffer solution (PBs) plus 3% sucrose as protective agent.

[0048] Labeled antibody: C-reactive protein (CRP) detection antibody 2 and control reagent 2 (chicken IgY) were fluorescently labeled with latex, stored in storage solution, and used for later use (50mMol / l Tris, 0.5% BSA, pH 7.8).

[0049] Marker preparation: Spray 5 microliters of the above-mentioned labeled antibody into the inner wall of the...

Embodiment 3

[0059] Example 3. (there is a marker pad, the coating reagent is an antigen, and there is no sample diluent)

[0060] Coating antibody: HCV (hepatitis C) detection antigen 1 was diluted to a fixed concentration (3.0mg / ml) with coating buffer, and the control reagent 1 (streptavidin SA) was diluted to a fixed concentration (2.0mg / ml ), using BioDot’s XYZ3060 to coat the above two liquids on a nitrocellulose membrane (NC membrane), wherein the quality control zone is located between the detection zone and the sample hole, and dried in a 37° C. oven for 4 hours. Coating buffer is 0.01Mol / l phosphate buffer solution (PBs) plus 3% sucrose as protective agent.

[0061] Labeled antibody: HCV (hepatitis C) detection antigen 2 and control antibody 2 (BSA-Biotin) were labeled with colloidal gold and stored in gold-labeled storage solution (50mMol / l Tris, 0.5% BSA, pH 7.8),

[0062] Marker pad preparation: Soak or spray the above-mentioned labeled antibody in or on the gold label pad ac...

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Abstract

The invention discloses an immune lateral chromatographic detection system as well as a preparation method and application thereof, and belongs to the field of medicine. The detection system comprises a substrate, wherein the substrate comprises a near-sample end and a far-sample end; the substrate is provided with a sample pad, a nitrocellulose membrane and a water-absorbing pad in sequence from the near-sample end to the far-sample end; the nitrocellulose membrane is provided with a quality control belt and a detection belt; the quality control belt is arranged between the detection belt and the sample pad; the quality control belt is a biotin-avidin system or a quality control system different from the species of a mouse antibody; the nitrocellulose membrane is provided with a view window used for collecting data. The detection system provided by the invention has higher sensitivity, precision and accuracy.

Description

technical field [0001] The invention relates to the medical field, in particular to an immune lateral flow detection system and its preparation method and application. Background technique [0002] The principle of immunological lateral flow chromatography is to fix the specific antibody (antigen) on a certain zone of the nitrocellulose membrane first, and when one end of the dry nitrocellulose membrane is immersed in the sample (urine, serum, plasma, whole blood or other sample), due to capillary action, the sample will move forward along the membrane, and when it moves to the area where the antibody (antigen) is immobilized, the corresponding antigen (antibody) in the sample will specifically bind to the antibody (antigen) If immune colloidal gold is used, the area can display a certain color, which can be observed with naked eyes or with a corresponding reader to achieve specific immunodiagnosis. If fluorescent markers are used, a supporting reader is required to complet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/543
CPCG01N33/558G01N33/543
Inventor 王龙
Owner 北京康思润业生物技术有限公司
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