Transplanting and acclimatizating method of virus-free tissue culture seedlings of strawberry pollen
A technology for tissue culture seedlings and strawberries is applied in the field of transplantation and domestication of strawberry pollen detoxification tissue culture seedlings, which can solve the problems of decreased survival rate, reduced seedling resistance, short time, etc., and achieves improved production efficiency, reduced production cost and Labor cost, the effect of shortening the cycle
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[0032] The transplanting acclimatization method of strawberry pollen detoxified tissue culture seedling comprises the following steps:
[0033] 1) Extraction and cleaning and disinfection of strawberry pollen:
[0034] Use strawberry pollen grown to 4mm-6mm in the single-nucleate anther stage in the field. The shape of strawberry flower buds at the pollen single-nuclei stage is: the calyx is not open, the flower bud is slightly longer than the corolla or the corolla is just white, the corolla is white or light green and not loose 1. The anthers are slightly yellow and full; after storing the fresh anthers at 2°C to 4°C for 24 hours, rinse the surface of the anthers with tap water; move the washed anthers into a sterile beaker on a super-clean workbench, First disinfect with 75% alcohol for 30 s to 40 s, rinse with sterile water 2 to 3 times, then sterilize with 0.1% mercuric chloride solution for 4 min to 5 min, gently stir in one direction with a sterilized glass rod, Then r...
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