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Non-antibiotic feed prepared through microbial fermentation

A technology of microbial fermentation and antibiotic-free feed, which is applied in the field of livestock antibiotic-free feed and its application, to achieve the effect of improving pig diarrhea, reasonable compatibility and remarkable curative effect

Active Publication Date: 2016-10-12
浙江伊杰斯生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] At present, the application of the combination of Lactobacillus paracasei, Aspergillus oryzae and Candida utilis as an antibiotic-free feed additive for pigs has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, the preparation of Lactobacillus paracasei bacterium liquid

[0034] Lactobacillus paracasei (strain number GMNL-33) was inoculated into an anaerobic tube containing MRS medium, and cultured anaerobically at a constant temperature of 37° C. for 48 hours to obtain Lactobacillus paracasei bacteria liquid.

[0035] The MRS medium is: peptone 10.0g / L, beef extract 8.0g / L, yeast extract 4.0g / L, glucose 20.0g / L, Tween 80 1ml / L, dipotassium hydrogen phosphate 2.0g / L, three Sodium acetate water 5.0g / L, triammonium citrate 2.0g / L, magnesium sulfate heptahydrate 0.2g / L, manganese sulfate 0.05g / L, pH 6.2-6.6.

Embodiment 2

[0036] Embodiment 2, the preparation of aspergillus oryzae liquid

[0037] Aspergillus oryzae (strain number CICC40188) was inoculated onto Czapek's agar medium, cultured at 28°C for 72h, then inoculated into Czapek's liquid medium, and incubated at 28°C for 72h to obtain Aspergillus oryzae bacterial liquid.

[0038] The Czapek's agar medium is: 3% sucrose, 0.3% sodium nitrate, 0.05% magnesium sulfate heptahydrate, 0.05% potassium chloride, 0.001% ferrous sulfate tetrahydrate, 0.1% potassium hydrogen phosphate, 1.5% agar, pH Value 6.0-6.5. Czapek's liquid medium without adding agar.

Embodiment 3

[0039] Embodiment 3, the preparation of Candida utilis bacterium liquid

[0040] Candida utilis (strain number ATCC22023) was inoculated on wort agar medium, cultured at 28° C. for 72 hours, then inoculated into wort liquid medium, and cultured at 28° C. for 72 hours to obtain Candida utilis strain.

[0041] The wort juice agar medium is as follows: the wort juice is 5-6 degrees Baume, the pH value is 6.5, and 2g / 100ml of agar is added. Wort juice liquid medium without adding agar.

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PUM

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Abstract

The invention relates to a non-antibiotic feed prepared by adding a mixture to a compound bacterium liquid and carrying out solid fermentation. The compound bacterium liquid is prepared by mixing 1-3 parts by weight of lactobacillus paracasei liquid, 1-3 parts by weight of aspergillus oryzae liquid and 1-3 parts by weight of candida utilis liquid. Compared with common pig feeds, the non-antibiotic feed has the advantages that the non-antibiotic feed is reasonable in compatibility, has obvious curative effects and can achieve the effect of obviously reducing the diarrhea rates of pigs; experiments prove that the non-antibiotic feed has special effects on improvement of diarrhea of pigs; without western medicines-antibiotics, the feed does not have toxic or side effect or drug resistance and is pollution-free.

Description

technical field [0001] The invention relates to the field of feed, in particular to an antibiotic-free feed for livestock and its application. Background technique [0002] Since the middle of the 20th century, adding antibiotics to feed has played a positive role in preventing animal diseases, promoting animal growth, increasing feed remuneration, and increasing the output of livestock and poultry products. With the widespread application of antibiotics as feed additives, its various disadvantages have also been recognized by people, such as causing the imbalance of normal flora in the gastrointestinal tract of animals and producing drug resistance. The long-term use of antibiotics leads to a large amount of residues in meat and egg products in animal production. These residues are toxic, teratogenic, carcinogenic, and mutagenic to humans. Not only the quality of animal products is lowered, but the residual poisons damage the environment and pose a threat to the health of ...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/37A23K20/142A23K20/147A23K20/174A23K20/26A23K50/30
CPCA23V2002/00A23V2250/76Y02P60/87
Inventor 潘宏涛卢亚萍
Owner 浙江伊杰斯生物科技有限公司
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