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Human bone morphogenetic protein-2 composite material and its preparation method and application

A technology of morphogenic proteins and composite materials, applied in the field of biomedicine, can solve problems such as high cost, clinical complications, and high doses

Active Publication Date: 2019-01-18
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a composite material comprising a porous carrier, BMP-2 and glucocorticoid drugs and its preparation method and application. Problems such as high doses during clinical use and the resulting high costs and clinical complications

Method used

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  • Human bone morphogenetic protein-2 composite material and its preparation method and application
  • Human bone morphogenetic protein-2 composite material and its preparation method and application
  • Human bone morphogenetic protein-2 composite material and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0138] The preparation of embodiment 1 porous bone-like apatite

[0139] 1 g of composite calcium phosphate salt powder composed of tetracalcium phosphate (TTCP) and anhydrous dicalcium phosphate (Dicalcium Phosphate Anhydrate, DCPA) in equimolar ratio, 1 g of NaCl particles (particle size about 500 μm), saturated NaCl solution 0.3g was mixed to obtain a uniform slurry, and the slurry was filled into a cylindrical mold with a diameter of 4mm and a height of 3mm and maintained at a pressure of 2MPa for 1min to obtain a scaffold body of a corresponding size and shape.

[0140] The stent embryo was cured in a constant temperature and humidity box at 37° C. and 100% humidity for 3 days, soaked in distilled water to completely dissolve NaCl, and then dried at 100° C. for 12 hours to obtain a porous bone-like apatite carrier.

[0141] Such as figure 1 As shown, the porous bone-like apatite carrier is a cylinder with a diameter of about 4 mm and a height of about 3 mm ( figure 1 A)...

Embodiment 2

[0143] Example 2 - Composite 1: 1 μg rhBMP-2 with 6 μg dexamethasone

[0144] Take 1 g of the porous bone-like apatite carrier described in Example 1 and carry out high-temperature and high-pressure steam sterilization. Under sterile conditions, add a solution containing 1 μg of rhBMP-2 and 6 μg of dexamethasone dropwise on the porous bone-like apatite placed on the carrier in a vacuum desiccator, vacuumed with a vacuum pump and maintained for 30 minutes, so that the solution is fully absorbed into the pores of the support.

[0145] Then the scaffold was taken out from the vacuum desiccator, and aseptically freeze-dried with a vacuum freeze dryer to obtain composite material 1. The immobilized amount of rhBMP-2 in composite material 1 was 1 μg, and it could be sealed at 4°C for long-term storage.

Embodiment 3

[0146] Example 3 - Composite 2: 1 μg rhBMP-2 with 3 μg dexamethasone

[0147] Same as Example 2, except that the amount of dexamethasone is 3 μg, and the immobilized amount of rhBMP-2 in composite material 2 is 1 μg.

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Abstract

The invention discloses a human bone morphogenetic protein-2 composite material and a preparation method and an application thereof. The composite material includes (a) a porous carrier, (b) a bone morphogenetic protein BMP-2 and (c) a glucocorticoid drug, and the (b) bone morphogenetic protein BMP-2 and the (c) glucocorticoid drug are loaded on the (a) porous carrier. The composite material can effectively accelerate the repair speed of bone tissues and improve the repair effect.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular, the invention relates to a composite material comprising a porous carrier, BMP-2 and glucocorticoid drugs, and a preparation method and application thereof. Background technique [0002] Bone tissue defect is an important clinical problem in orthopedics. There are more than 5 million patients with bone tissue defects in my country every year, among which the number of patients with fractures of the spine and extremities caused by osteoporosis is more than 2 million; about 40 patients need bone transplantation due to bone tumor resection and spinal fusion. Thousands of cases. Therefore, bone tissue defect and injury has become an important disease affecting people's health and life. [0003] Currently, autologous bone grafting is the gold standard for clinical treatment of bone defects. However, this method will bring great pain and surgical complications to patients, and there are outs...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/42A61L27/22A61L27/54A61L27/56A61K31/573A61P19/08
CPCA61K31/573A61L27/22A61L27/425A61L27/54A61L27/56A61L2300/252A61L2300/412A61L2300/43A61L2300/602A61L2430/02
Inventor 刘昌胜林丹柴延军袁媛
Owner EAST CHINA UNIV OF SCI & TECH