Recombinant alpha protein for inhibiting Clostridium perfringens infection and its preparation method and application

A technology for Clostridium perfringens and alpha protein, which is applied in the directions of antibacterial immunoglobulins, botanical equipment and methods, biochemical equipment and methods, etc., and can solve problems such as difficulty in increasing the renaturation rate.
CN106008684BInactive Publication Date: 2020-01-14CHINA ANIMAL DISEASE CONTROL CENT
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
CHINA ANIMAL DISEASE CONTROL CENT
Publication Date
2020-01-14
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses recombinant alpha protein for inhibiting clostridium perfringens infection and a preparation method and application thereof. The recombinant alpha protein is shown in (a) or (b) or (c), wherein the protein in (a) is composed of amino acid sequences shown as SEQ ID No.2; the protein in (b) is composed of amino acid sequences shown at the sites No.51-No.353 of SEQ ID No.2; the fusion protein in (c) is obtained by fusing protein tags at carboxyl terminals or / and amino terminals of the protein shown in (a) or (b). The recombinant alpha protein enables animals to have a higher serum antibody level and resist attack of clostridium perfringens after the animals are immunized with the recombinant alpha protein. The recombinant alpha protein is good in solubility and easy to purify and can serve as a diagnostic antigen to be prepared into a monoclonal antibody or be used for further research on protein functions and conformation relations.
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Description

technical field

[0001] The invention relates to a recombinant alpha protein for inhibiting Clostridium perfringens infection in the field of biotechnology, a preparation method and application thereof. Background technique

[0002] Clostridium perfringens (Clostridium Perfringens), also known as Clostridium welchii, is an important zoonotic disease. The pathogen is traumatic gas gangrene and human food poisoning, as well as sheep blight, lamb dysentery, and necrosis of cattle and sheep. It is one of the main pathogens of acute enteritis and cattle and sheep enterotoxemia, which has caused huge economic losses to animal husbandry. The main pathogenic factor of Clostridium perfringens is the exotoxin secreted by it. There are as many as 13 types, among which α, β and ε are the most important exotoxins. According to the different types of exotoxins produced, the perfringens can be Clostridium mycobacterium is divided into five serotypes A, B, C, D, and E. The prevention and c...

Claims

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