Rapid preparation method of high-purity superhelical structure type Ⅰ collagen
A collagen and supercoil technology, applied in peptide preparation methods, chemical instruments and methods, animal/human proteins, etc., can solve the problems of high preparation costs, cumbersome and complicated process, unclear collagen configuration, etc., to achieve The effect of saving production cost, ensuring integrity and improving yield
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Embodiment 1
[0047] The rapid preparation process of high-purity superhelical structure type Ⅰ collagen, the steps are as follows:
[0048] (1) Raw material pretreatment
[0049] Put 2 kg of washed American redfish scales into the reaction kettle, add an alkali solution 10 times the weight of the fish scales, stir for 1 hour, wash with water, add an acid solution 10 times the weight of the fish scales, stir for 1 hour, and then wash with water, wherein The mass volume concentration of the alkali solution is 3%, the mass volume concentration of the acid solution is 5%, and the pretreatment temperature is 25°C;
[0050] (2) Type Ⅰ collagen extraction and filtration
[0051] Add 0.5mol / L acetic acid as extractant to the pretreated raw material, the weight ratio of raw material and extractant is 1:3, and carry out homogenate extraction for 3 hours at the same time, the homogenate speed is 25000 rpm, with stirring Remove heat to maintain extraction temperature at 4°C. Since the particle size...
Embodiment 2
[0058] The rapid preparation process of high-purity superhelical structure type Ⅰ collagen, the steps are as follows:
[0059] (1) Raw material pretreatment
[0060] Put 5 kg of cleaned tilapia scales into the reaction kettle, add an alkali solution 8 times the weight of the scales, stir for 10 hours, wash with water, add an acid solution 8 times the weight of the scales, stir for 3 hours, and then wash with water, wherein The mass volume concentration of the alkaline solution is 2%, the mass volume concentration of the acid solution is 6%, and the pretreatment temperature is 20°C;
[0061] (2) Type Ⅰ collagen extraction and filtration
[0062]Add 0.25mol / L citric acid as extractant to the raw material after pretreatment, the weight ratio of raw material and extractant is 1:2, carry out the homogenate extraction of 2.5 hours simultaneously, homogenate speed is 10000 rev / min, with Stir to dissipate heat to maintain the extraction temperature at 6°C. Since the particle size o...
Embodiment 3
[0069] The rapid preparation process of high-purity superhelical structure type Ⅰ collagen, the steps are as follows:
[0070] (1) Raw material pretreatment
[0071] Put 0.5 kg of washed American redfish scales into the reaction kettle, add an alkali solution 6 times the weight of the fish scales, stir for 1 hour, wash with water, add an acid solution 6 times the weight of the fish scales, stir for 4 hours, and then wash with water. Wherein the mass volume concentration of alkali solution is 1%, the mass volume concentration of acid solution is 8%, and pretreatment temperature is 15 ℃;
[0072] (2) Type Ⅰ collagen extraction and filtration
[0073] Add 1 mol / L acetic acid as extractant to the pretreated raw material, the weight ratio of raw material and extractant is 1:1, and carry out homogenate extraction for 4 hours at the same time, the homogenate speed is 28000 rpm, with stirring to dissipate heat To maintain the extraction temperature at 8°C. Since the particle size o...
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