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Method for high-efficient production of cellulosic ethanol through using reeds

A technology of cellulosic ethanol and reed, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low efficiency, restrictions on large-scale commercial production of cellulosic ethanol, and high cost of ethanol, and achieve improved degradation efficiency effect

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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The cell wall has a complex composition and a dense structure, which can resist the hydrolysis of cellulose by cellulase, making the efficiency of cellulose hydrolysis to produce sugar low, and the cost of fermentation to produce ethanol is high, which limits the large-scale commercial production of cellulosic ethanol

Method used

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  • Method for high-efficient production of cellulosic ethanol through using reeds
  • Method for high-efficient production of cellulosic ethanol through using reeds
  • Method for high-efficient production of cellulosic ethanol through using reeds

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Embodiment Construction

[0045] Below in conjunction with accompanying drawing and embodiment describe in detail:

[0046] 1. Comparison table of experimental results

[0047]

[0048] From this it can be seen that the method can be used to efficiently produce cellulose ethanol with reeds as raw materials.

[0049] Two, the embodiment

[0050] 1) Solution preparation

[0051] 1. Sample preservation solution (CTAB solution) (in step ②)

[0052] 1) CTAB, according to the mass / volume ratio: 3%, that is, 3g / 100 mL;

[0053] 2) Tris-HCl, pH=8.0: 100mM;

[0054] 3) EDTA, pH=8.0: 20 mM;

[0055] 4) NaCl: 1.4M;

[0056] 5) PVP-30, according to mass / volume ratio: 1%, that is, 1g / 100 mL, add after sterilization;

[0057] 6) β-Mercaptoethanol, by volume / volume ratio: 0.2-0.6%, add just before use.

[0058] Preparation method:

[0059] A. After dissolving and mixing the first four components with ultrapure water, adjust the pH to 8.0 (1M NaOH and 1M HCl to adjust the pH), constant volume, and then pla...

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Abstract

The invention discloses a method for producing cellulosic ethanol, and concretely relates to a method for high-efficient production of cellulosic ethanol through using reeds. The method comprises the following steps: chopping reeds, and adding water to adjust; 2, carrying out water vapor explosion pretreatment; 3, collecting residues obtained after vapor explosion; 4, carrying out calcium oxide pretreatment; 5, adjusting the pH value; 6, carrying out cellulase hydrolysis; 7, fermenting; and 8, collecting ethanol. Compared with the prior art, the method combining the vapor explosion pretreatment technology and the calcium oxide pretreatment technology has the following advantages: the cellulose degradation efficiency is substantially improved; a surfactant tween-80 is added in the cellulase hydrolysis process, so the cellulose degradation efficiency is further improved; the reeds are adopted as a raw material, and the above technology combination is adopted to convert 98% of celluloses in the raw material into glucose; and when the technology combination is used in production of cellulosic ethanol, 190g of ethanol can be produced from every kilogram of the reeds (dry weight), so the ethanol yield reaches up to 19%.

Description

technical field [0001] The invention relates to a method for producing cellulose ethanol, in particular to a method for efficiently producing cellulose ethanol with reeds. Background technique [0002] With the gradual depletion of fossil energy and the deterioration of the environment, vigorously promoting the use of renewable energy has become the main energy development strategy of all countries in the world. Bioenergy is an important renewable energy source, because of its rich resources and less environmental pollution, it has a broad market prospect. Cellulosic ethanol refers to ethanol produced from cellulose in the cell walls of crop straw or plant stems, and is a bioenergy with important development prospects. [0003] Plant cell walls are mainly composed of cellulose, hemicellulose and lignin. The complex composition and compact structure of the cell wall can resist the hydrolysis of cellulose by cellulase, making the efficiency of cellulose hydrolysis to produce...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12P19/14C12R1/865
CPCC12P7/10C12P19/14C12P2201/00Y02E50/10
Inventor 彭良才夏涛涂媛苑金文祥陈灵