Molecularly imprinted electrochemical sensor for detecting trace sulfamonomethoxine

A technology of sulfamethoxine and molecular imprinting, applied in the direction of material electrochemical variables, etc., can solve the problems that there is no relevant research on trace amount of sulfamethoxine, and achieve easy automation of detection, good reproducibility and stability , Improve the effect of sensitivity and selectivity

Inactive Publication Date: 2016-10-12
ZHAOQING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Molecular imprinting is a molecular-specific recognition technology that has emerged in recent years. Combining molecular imprinting technology with electroanalytical chemistry technology can o

Method used

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  • Molecularly imprinted electrochemical sensor for detecting trace sulfamonomethoxine
  • Molecularly imprinted electrochemical sensor for detecting trace sulfamonomethoxine
  • Molecularly imprinted electrochemical sensor for detecting trace sulfamonomethoxine

Examples

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Embodiment 1

[0020] Example 1: Construction of the molecularly imprinted electrochemical sensor for detecting trace amounts of sulfamethoxine according to the present invention, characterized in that the molecularly imprinted electrochemical sensor is prepared by the following method:

[0021] (1) Pretreatment of glassy carbon electrodes

[0022] The glassy carbon electrodes were sequentially coated with 0.5 μm and 0.05 μm Al 2 o 3 The powder was polished on suede, rinsed with ultrapure water, and then ultrasonically cleaned in ethanol and water for 10 minutes, and then the electrode was scanned in potassium ferricyanide until a reversible cyclic voltammetry peak was obtained.

[0023] (2) Preparation of rGO (reduced graphene oxide) suspension

[0024] Take 0.002g of rGO and 2mL of dimethylformamide in a 10mL beaker, ultrasonically disperse for 2h to obtain a uniformly dispersed rGO suspension dispersion, and store it in a refrigerator at 6°C until use.

[0025] (3) Construction of mole...

Embodiment 2

[0027] Example 2: Cyclic voltammetry characterization of the molecularly imprinted electrochemical sensor for detecting trace amounts of sulfamethoxine according to the present invention

[0028] Since the imprinted holes in the membrane can serve as channels for electron transfer, using K 3 [Fe(CN) 6 ] as a probe, according to the magnitude of its current to characterize the surface properties of various electrodes. like figure 1 shown, different electrodes at 5mM K 3 [Fe(CN) 6 ] The cyclic voltammograms in the solution are significantly different. Curve a is the cyclic voltammogram of the bare electrode, with obvious redox peaks. As shown in curve b, when a layer of rGO composite is modified on the surface of the glassy carbon electrode, the peak current increases significantly, indicating that the modification of the rGO composite can promote electron transfer on the electrode surface and increase the current signal. As shown in curve c, when the MIP film is formed, t...

Embodiment 3

[0029] Example 3: Scanning Electron Microscopy Characterization of Molecularly Imprinted Electrochemical Sensing for Detection of Trace Sulfamethoxine According to the Present Invention

[0030] The microstructure of different modified electrodes was characterized by scanning electron microscopy. like figure 2 As shown in a, the surface of the electrode is smooth after the molecular imprinted membrane is modified on the electrode surface, indicating that the imprinting effect is good; after elution, the template molecules are eluted, leaving many holes on the surface of the molecularly imprinted membrane, so the surface becomes rough (see figure 2 b), so there will be a current response peak when scanning.

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Abstract

The invention relates to a molecularly imprinted electrochemical sensor for detecting trace sulfamonomethoxine. The molecularly imprinted sensor for sulfamonomethoxine (SMM) is prepared by electropolymerization with a cyclic voltammetry by taking sulfamonomethoxine as template molecules and dopamine as functional monomer, an electrochemical signal is enhanced by modification with reduced graphene oxide (rGO), and a detection limit of the method is improved. In a phosphate buffered solution of which the pH is 8, potassium ferricyanide is taken as a probe, a differential pulse voltammetry is taken as an electrochemical excitation signal, and research results display that the peak current difference and the SMM concentration have a favorable linear relation within a range of 20.0 to 1600.0nM, wherein r is equal to 0.9964, and the detection limit is 1.0nM. The sensor disclosed by the invention is used for detection of an actual sample with favorable reproducibility and stability, and the recovery rate reaches 92.4 to 97.9 percent.

Description

technical field [0001] The invention belongs to the technical field of novel functional materials and electrochemical sensing and detection, and relates to a molecularly imprinted electrochemical sensor, in particular to a molecularly imprinted electrochemical sensor for detecting trace amounts of sulfamethoxine. Background technique [0002] Sulfonamides are synthetic p-aminobenzenesulfonamide derivatives, which were first discovered in 1935. Afterwards, various antibiotics were continuously discovered and gradually replaced sulfa drugs clinically, but sulfa drugs still have their unique advantages: broad antibacterial spectrum, stable properties, low price, and mass production in China. In particular, the emergence of antibacterial synergists has expanded the antibacterial spectrum and enhanced antibacterial activity of sulfa drugs after combined use. Therefore, sulfonamides are still important drugs for anti-infection treatment in animal husbandry and aquaculture. Among ...

Claims

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Application Information

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IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 汪洪武刘艳清韦寿莲姚夙严子军张吟芬
Owner ZHAOQING UNIV
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