Extraction method of dendrobium huoshanense polysaccharide

Abstract

The invention provides an extraction method of dendrobium huoshanense polysaccharide, and in particular relates to a method for extracting dendrobium huoshanense polysaccharide by an ultrasonic wave assistance hot reflux extraction method. According to the extraction method, the extracted polysaccharide is subjected to content detection by a phenol-sulfuric acid method. After the primary impurity removal through diethyl ether addition into dendrobium huoshanense coarse powder, a proper amount of methyl alcohol is added; after ultrasonic wave assistance hot reflux extraction, filtering is performed when the liquid is still hot; washing is performed; filtering residues are put into a flask; a proper amount of water is added; then, ultrasonic wave assistance hot reflux extraction is performed again; filtering is performed when the liquid is still hot; the operations are repeated for four times; obtained filter liquid and washing liquid are merged; through rotary evaporation, a target product, namely the dendrobium huoshanense polysaccharide is obtained. The extraction method of the dendrobium huoshanense polysaccharide provided by the invention has the advantages that the content of the obtained polysaccharide is high; the extraction time is short; the extraction is thorough; the extraction method is suitable for industrial application.

Description

technical field [0001] The invention relates to a method for extracting polysaccharides from Dendrobium huoshanense. Background technique [0002] Dendrobium is a plant of the genus Dendrobium in the family Orchidaceae. It is a precious medicinal material in traditional Chinese medicine. Its active ingredients are mainly polysaccharides and alkaloids. Among them, the quality of Dendrobium depends largely on the content of polysaccharides in Dendrobium. Among the Dendrobium species, Dendrobium Huoshanense is the most famous. The pharmacological effects of polysaccharides in Dendrobium Huoshanense mainly include immunity, strength, anti-aging, and anti-tumor effects. With the development of society, people pay more and more attention to health, and its demand will also increase. However, the traditional method of extracting polysaccharides from Dendrobium huoshanense is to extract them from the roots, stems, leaves and other plants of Dendrobium huoshanense , the products obt...

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Problems solved by technology

[0004] Chinese Invention Patent Application No. 200810162496.7 discloses a preparation method of a polysaccharide extract of Dendrobium nobile, which is subjected to degreasing, extraction, alcohol precipitation, protein removal, monosaccharide and oligosaccharide removal, coagulation The gel column purification and other steps are realized, and the polysaccharide content in the obtained polysaccharide extract is more than 90%, but this method is complicated to operate, has many processes, and takes a long time, so it is not suitable for industrial application

[0005] No. 200810162496.7 of China Invention Patent Application Publication describes a kind of hypoglycemic active dendrobium polysaccharide and its extraction method. The crushed dry dendrobium is selected, and 20 to 40 times the weight of water is added, and the amount of cellulase added is 10 to 40 times. 20g / mg, enzymatic hydrolysis PH is 4.5~5.5, first use ultrasonic-enzymolysis co-extraction, enzymolysis temperature 45~55℃, extraction time 10~20min, then heat up to inactivate the enzyme to obtain Dendrobium polysaccharide extract, the process of the invention is simple , The time is short, which improves the yield and purity of dendrobium polysaccharides, but the amount of cellulase used in this method is more, and the pH is 4.5~5.5, which will affect the structure of polysaccharides, and is not suitable for industrial applications