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A gene Gbabc

A gene and gene sequence technology, applied in genetic engineering, plant gene improvement, peptide source, etc., can solve problems that are difficult to apply to clinical diagnosis, and achieve intuitive and reliable results, high specificity and sensitivity

Active Publication Date: 2020-05-08
HEBEI AGRICULTURAL UNIV.
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These detection methods have high requirements on operation steps, operation environment, and equipment, and are difficult to apply to clinical diagnosis.

Method used

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  • A gene Gbabc
  • A gene Gbabc
  • A gene Gbabc

Examples

Experimental program
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Effect test

Embodiment Construction

[0024] In this experiment, DNAstar was used to predict and analyze the main antigen glycoprotein gB of bovine infectious rhinotracheitis virus, select 3 epitope genes with high antigen index, and find 3 corresponding gene sequences according to the starting positions of the 3 amino acid sequences , and named as gBa, gBb and gBc respectively, the three gene sequences were connected by Linker sequence, and EcoR I and XhoI were selected as restriction sites, and the fused gene sequences were sent and synthesized to obtain the recombinant cloning vector PUCK- (gBa-linker-gBb-linker-gBc). The recombinant cloning vector exists in Escherichia coli DH-5α, and the target gene is cloned and amplified by shaking bacteria, and the target gene gBabc with sticky ends is obtained by plasmid extraction, double enzyme digestion and gel recovery (the nucleotide sequence is as follows: List SEQ ID NO: 1), connect it with the linearized expression vector pET-28a(+) to obtain the recombinant proka...

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Abstract

The invention discloses a gene gBabc which is obtained from main antigen glycoprotein gB of infectious bovine rhinotracheitis virus. The gene can express fusion protein which is high in immunogen activity, and finally, the gene can make a colloidal gold immunochromatography test strip which is used for detecting an infectious bovine rhinotracheitis antibody. The target gene disclosed by the invention, through successful and efficient expression as well as separation and purification, can prepare fusion antigen protein gBF for detecting the infectious bovine rhinotracheitis antibody, and experiments prove that the fusion protein is high in antigenicity and immunocompetence; therefore, a colloidal gold immunochromatography for the infectious bovine rhinotracheitis antibody is established.

Description

technical field [0001] The invention relates to a target gene gBabc obtained from the main antigen glycoprotein gB of bovine infectious rhinotracheitis virus. Background technique [0002] Infectious bovine rhinotracheitis (IBR) is an acute, febrile, Contact infectious diseases, clinical symptoms include inflammation of the upper respiratory tract and tracheal mucosa, dyspnea, runny nose, etc., accompanied by conjunctivitis, abortion, mastitis, and sometimes calf encephalitis. The more serious harm is to cause persistent infection, which causes great difficulties in diagnosis, prevention and treatment of the disease. The outermost envelope of bovine infectious rhinotracheitis virion is composed of 11 kinds of glycoproteins, among which gB, gC and gD glycoproteins are highly immunogenic, which can stimulate the body to produce antibodies and cause lysis of infected cells in the presence of complement. [0003] At present, the differential diagnosis methods of IBR mainly inc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/38C07K14/06C07K19/00C12N15/70G01N33/569
CPCC07K14/005C07K2319/00C12N2710/16022G01N33/56994G01N2333/06
Inventor 秦建华刘腾赵月兰史万玉包永占景翠王敏赵越刘立元
Owner HEBEI AGRICULTURAL UNIV.
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