Biophoton spectrum detection system and method

A biophotonic and spectral detection technology, applied in the field of biological and biomedical technology imaging, can solve the problems of insufficient attention, weak biophoton intensity, and no biophoton detection methods, and achieve rich functions, diverse choices, and good quality. The effect of scalability and upgrade potential

Inactive Publication Date: 2016-10-26
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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Problems solved by technology

However, this kind of biodetector did not receive enough attention at that time, and because the biophoton intensity was too weak and limited by the technology at that time, no more sensitive biophoton detection method appeared.

Method used

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  • Biophoton spectrum detection system and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0037] Example: Glutamate-induced biophotonic spectroscopy in mouse brain slices

[0038] 1. Test materials: adult Kunming mice, from the Public Service Center for Experimental Animals of Hubei Province.

[0039] 2. Detection steps:

[0040] (1) Test preparation: Before starting the test, first turn on the air conditioner to keep the laboratory room temperature at about 25°C, then use ultrapure water to clean the brain slice perfusion system, and turn on the EM-CCD and low-temperature coolant circulation pump to make the The EM-CCD is preheated in a working environment of -95°C.

[0041] (2) Laser calibration: Three lasers with different wavelengths of 405nm, 532nm and 650nm are used to calibrate the biophoton detection system. Moreover, the laser light source needs to be calibrated at normal intensity and biophoton intensity respectively.

[0042] The calibration results are attached Figure 2a As shown, the upper figure is the laser spectrum at normal intensity, and the ...

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Abstract

The present invention provides a biophoton spectrum detection system and method. The biophoton spectrum detection system is characterized by adding a sub-photon system composed of slits and a grating on the basis of an ultraweak biophoton imaging system. The grating is an optical element composed of a lot of parallel slits of equal widths and equal intervals, and enables the light to generate chromatic dispersion by utilizing the multi-slit diffraction principle. According to the multi-slit diffraction principle, the ultraweak biophotons emitted out by a to-be-detected sample form a line light source after passing the slits, the line light source irradiates the grating and then is diffracted by the multiple slits to form the bright fringes which are arranged at equal intervals, and the distance is relevant with the wavelength of the biophotons. An imaging subsystem is used for the image recording and analysis. The experiment detection proves that the biophoton spectrum detection system of the present invention is high in sensitivity, can detect the spectrums of the ultraweak luminescence of the biologies, at the same time, has the characteristics of being simple and convenient in operation, good in expansibility and abundant in function, and can be widely used in the fields, such as the life science research, the medical clinical examination, the agriculture production, the food safety, the environment protection, etc.

Description

technical field [0001] The invention relates to a method of using ultra-weak biophoton radiation signals to perform biophoton spectrum detection on organisms (individuals, tissues, cells and molecules, etc.) to realize biophoton spectrum imaging, which belongs to the field of biological and biomedical technology imaging. This technology can also be applied to other fields that require long-term imaging for weak-light spectral analysis. Background technique: [0002] Biophoton is the abbreviation of biological ultra-weak luminescence. In essence, it is no different from the usual light. The spectrum range is 200-800nm, and it is commonly found in various living organisms such as microorganisms, plants and animals. Biophotonic activity can sensitively reflect the physiological state of an organism. [0003] From the perspective of molecular physics, biophotons can be understood as the energy released by biomolecules during the transition from a high-energy state to a low-ener...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01J3/28G01J3/02G01N21/25G01N21/27
CPCG01J3/0243G01J3/2803G01J3/2823G01N21/255G01N21/27G01N21/274G01J2003/282G01J2003/006G01N2201/068G01J3/28G01N21/25
Inventor 戴甲培王卓李泽华
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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