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Application of Cordyceps sinensis in the preparation of medicines for preventing and/or treating yellow fever virus infection

A technology for Cordyceps sinensis and yellow fever virus, which is applied in the field of medicine and achieves the effects of small side effects, significant effect and quick effect.

Active Publication Date: 2020-04-07
青海珠峰冬虫夏草工程技术研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no literature has reported that it has the effect of preventing and treating yellow fever virus infection

Method used

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  • Application of Cordyceps sinensis in the preparation of medicines for preventing and/or treating yellow fever virus infection
  • Application of Cordyceps sinensis in the preparation of medicines for preventing and/or treating yellow fever virus infection
  • Application of Cordyceps sinensis in the preparation of medicines for preventing and/or treating yellow fever virus infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Preparation of Water Extract of Cordyceps Fermented Mycelium

[0018] The mycelium of Cordyceps sinesis strain Hirsutela sinensis was cultured by liquid fermentation, extracted with hot water, centrifuged and filtered, concentrated under reduced pressure, and freeze-dried to obtain the water extraction of the fermented mycelium of Cordyceps sinesis Things.

Embodiment 2

[0019] Example 2 Toxicity test of water extract of Cordyceps fermented mycelium on Vero cells

[0020] Vero cells are susceptible cells of fever virus. The cytotoxicity of the water extract of the fermented mycelium of Cordyceps sinensis to Vero cells needs to be tested first, so as to provide data reference for subsequent inhibition experiments on fever virus.

[0021] The experimental steps are as follows:

[0022] 1: Inoculate Vero cells: prepare a single cell suspension with DMEM medium containing 10% (v / v) fetal bovine serum, inoculate a 96-well cell culture plate with 5000-8000 cells per well, with an inoculation volume of 200 μL per well ;

[0023] 2: Culture Vero cells: at 37°C, 5% (v / v) CO 2 Cultivate for 48 hours under cultivation conditions;

[0024] 3: Add the water extract of the fermented mycelium of Cordyceps sinensis: aspirate the DMEM medium in each well, add 200μL to each well and dilute to the corresponding concentration with DMEM medium containing 10% (v / v) fetal bo...

Embodiment 3

[0030] Example 3 Toxicity test of high concentration aqueous solution of Cordyceps sinensis on Vero cells

[0031] According to the experimental method of Example 2, the cytotoxicity of the high concentration aqueous solution of Cordyceps sinensis on Vero cells was detected.

[0032] Among them, in step 2, add 200 μL to each well and dilute to 100 mg·L with DMEM medium containing 10% (v / v) fetal bovine serum -1 A high-concentration aqueous solution of Cordyceps sinensis (Ultra-fine powder of Cordyceps sinensis is made by ultrafine pulverization); 200μL DMEM medium without 10% (v / v) fetal bovine serum of Cordyceps sinensis is added to the control wells (see Table 2 ).

[0033] Table 2 Toxicity of high concentration aqueous solutions of Cordyceps sinensis to Vero cells

[0034]

[0035] It can be seen that even if the aqueous solution of Cordyceps is 100mg·L -1 Under the high-dose concentration conditions, the survival rate of Vero cells can still reach 91.49%, indicating that its cytot...

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Abstract

The invention relates to pharmaceutical application of Cordyceps sinensis and provides application of Cordyceps sinensis in the preparation of drugs for preventing and / or treating yellow fever virus infections; the drugs include Cordyceps sinensis as an active ingredient and one or more pharmaceutically acceptable excipients, the Cordyceps sinensis is selected from natural Cordyceps sinensis, fermented Cordyceps sinensis mycelia and extract of the natural Cordyceps sinensis and fermented Cordyceps sinensis mycelia, and the extract is one, or a combination of any, of aqueous extract, alcohol extract, ester extract, ether extract and chloroform extract. Inhibition experiments on yellow fever virus prove that Cordyceps sinensis has good inhibition effect, and therefore it is proved that Cordyceps sinensis may be used to prevent and / or treat yellow fever virus infections and has the advantages such as significant effect, low side effect and high acting speed. Therefore, Cordyceps sinensis can be used to develop drugs for treating diseases due to yellow fever virus and has great social benefit and research value.

Description

Technical field [0001] The invention belongs to the field of medicine, and relates to the medicinal use of Cordyceps sinensis, in particular to its use in preparing medicines for preventing and / or treating yellow fever virus infection. Background technique [0002] Yellow fever virus (YFV) belongs to the yellow fever virus genus of the yellow fever family. It is an RNA virus and has visceral and neurotrophic properties. Yellow fever virus can cause yellow fever, which is an acute infectious disease. Yellow fever is mainly transmitted by mosquito vectors. The main clinical features are high fever, jaundice, proteinuria and hemorrhage. The course of the disease is about two weeks, and the case fatality rate is as high as about 20-50%. Internationally, yellow fever has been designated as a quarantine infectious disease, and China has also designated it as a Class A infectious disease. The disease is mainly endemic in tropical areas of Central and South America and Africa, and it i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/068A61P31/14
CPCA61K36/068Y02A50/30
Inventor 王玉花王辉韩贵香夏敏贞王丽罗成军
Owner 青海珠峰冬虫夏草工程技术研究有限公司
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