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Avian influenza virus strain

An avian influenza virus and vaccine technology, applied in the directions of viruses, antiviral agents, virus antigen components, etc., can solve problems such as uncontrollable epidemic development, and achieve the effects of good safety, good immunogenicity and high titer

Active Publication Date: 2016-11-09
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The variation of avian influenza virus among poultry epidemic viruses is relatively the most frequent, so that although a variety of vaccines have been introduced, the development of the epidemic is still uncontrollable, so it is necessary to screen and obtain new epidemic strains to deal with the mutation. new hazards

Method used

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  • Avian influenza virus strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: Screening of H9 subtype avian influenza virus strain

[0019] 1. Epidemiological survey Since the discovery of H9 subtype avian influenza in northern my country in 1998, we have been tracking the prevalence of H9 subtype avian influenza and virus mutation. In recent years, avian influenza has appeared in many areas Symptoms or dead chickens, after a large number of epidemiological investigations, in 2015, we successfully isolated a strain of H9 subtype avian influenza virus from chickens in a chicken farm in Fujian.

[0020] 2. Virus isolation AIV was isolated according to the method reported in the literature. Add the disease material to the normal saline containing double antibody at a ratio of 1:5 (w / v), grind it to make a suspension, centrifuge at 1000r / min for 5 minutes, take the supernatant and inoculate the allantoic cavity of 10-11-day-old SPF chickens Embryos, 0.2ml per embryo, hatched at 36°C-37°C for 3-4 days, irradiated the embryos twice a day,...

Embodiment 2

[0030] Embodiment 2: Preparation of H9 subtype avian influenza antigen

[0031] 1 Inoculation Take the virus seed for production and make appropriate dilution with sterilized physiological saline (such as 10 -3 or 10 -4 ), according to the "Instructions for the Use of Automatic Inoculation Machine", inoculate 10-11-day-old susceptible chicken embryos, 0.1ml per embryo, seal the pinhole after inoculation, and continue incubation at 36-37°C without turning the embryos.

[0032] 2 Incubation and observation After inoculation of the chicken embryos, the embryos were illuminated once a day, and the chicken embryos that died 48 hours ago were discarded. Thereafter, the embryos were photographed once every 4-6 hours, and the dead chicken embryos were taken out at any time until 96 hours, no matter whether they were dead or not, all of them were taken out, the air chamber was upright, and cooled at 2-8°C for 12-24 hours.

[0033] 3 Harvesting Take out the cooled chicken embryos, and...

Embodiment 3

[0040] Embodiment 3: the preparation of vaccine

[0041] 1 Oil phase preparation Take 95 parts of mineral oil and 1 part of aluminum stearate, mix them evenly in the oil phase preparation tank and heat to 80°C, add 5 parts of Siben-80, and keep it for 40 minutes when the temperature reaches 115°C , and set aside after cooling.

[0042] 2 Preparation of the aqueous phase Mix the inactivated H9 subtype avian influenza virus venom at a ratio of 1:2. Take 95 parts of mixed antigen solution and 5 parts of sterilized Tween-80, and mix well to completely dissolve Tween-80.

[0043] 3 Emulsification Take 2 parts of the oil phase and put them into the emulsification tank, start the motor, and stir at a slow speed. At the same time, slowly add 1 part of the water phase, and then stir at 3500r / min for 30-40 minutes. After emulsification, take 10ml of the vaccine and put it into a centrifuge tube, centrifuge at 3000r / min for 15 minutes, and the water precipitated at the bottom of the tu...

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Abstract

The invention aims to provide an avian influenza virus strain. The avian influenza virus strain has a preservation number of CCTCC No.V201617. The new H9 subtype avian influenza virus strain FJ 11 provided by the invention is high in titer of HA and EID<50> (egg infectious dose), good in immunogenicity, and can withstand attacks of local epidemic isolated virus of the H9 subtype avian influenza virus. The prepared vaccine is good in safety, and is free of any local or systemic adverse reactions caused by the vaccine. In the preservation period test, analysis of data of shape and properties, a safety test and a potency test is performed, the results show that all indexes are stable and effective compared with similar products.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to an avian influenza virus strain. Background technique [0002] Avian influenza is one of the severe infectious diseases of poultry. It is caused by type A influenza virus. When poultry is infected with H9 subtype avian influenza virus, especially when it is accompanied by mixed infection, it can lead to high mortality and a serious drop in egg production rate. Moreover, it is an acute and highly contagious disease with high mortality and high mortality, which is mainly popular in animals and humans. It occurs widely in all parts of the world and is very harmful to humans and the poultry industry. [0003] The variation of avian influenza virus among poultry epidemic viruses is relatively the most frequent, so that although a variety of vaccines have been introduced, the development of the epidemic is still uncontrollable, so it is necessary to s...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K39/145A61P31/16
CPCA61K39/12A61K2039/5252A61K2039/552A61K2039/55566C12N7/00C12N2760/16021C12N2760/16034
Inventor 李陆梅宋新宇马爽宫晓刘东郑欢郭伟伟
Owner YEBIO BIOENG OF QINGDAO
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