Avian influenza virus strain

An avian influenza virus and vaccine technology, applied in the directions of viruses, antiviral agents, virus antigen components, etc., can solve problems such as uncontrollable epidemic development, and achieve the effects of good safety, good immunogenicity and high titer

Active Publication Date: 2016-11-09
YEBIO BIOENG OF QINGDAO
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The variation of avian influenza virus among poultry epidemic viruses is relatively the most frequent, so that although a variety of vaccines have been intro

Method used

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  • Avian influenza virus strain

Examples

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Example Embodiment

[0018] Example 1: Screening of H9 subtype avian influenza strains

[0019] 1. Epidemiological investigation Since the discovery of H9 subtype avian influenza in northern my country in 1998, we have been tracking the prevalence and virus variation of H9 subtype avian influenza. In recent years, avian influenza has appeared in many areas Symptomatic or dead chickens, after a large number of epidemiological investigations and studies, in 2015, we successfully isolated a strain of H9 subtype avian influenza virus from chickens in a chicken farm in Fujian.

[0020] 2. Virus isolation According to the method reported in the literature, AIV was isolated. The diseased material was added to the physiological saline containing double antibody at a ratio of 1:5 (w / v), ground to make a suspension, centrifuged at 1000 r / min for 5 minutes, and the supernatant allantoic cavity was taken to inoculate SPF chickens aged 10 to 11 days. Embryos, 0.2ml per embryo, incubated at 36℃~37℃ for 3~4 days...

Example Embodiment

[0030] Example 2: Preparation of H9 subtype avian influenza antigens

[0031] 1. Inoculation Take the poisonous seeds for production and dilute them appropriately with sterile normal saline (such as 10 -3 or 10 -4 ), inoculate 10-11-day-old susceptible chicken embryos, 0.1 ml per embryo, seal the pinholes after inoculation, and continue to incubate at 36-37°C without turning the embryos.

[0032] 2 Incubation and observation After inoculation of chick embryos, the embryos were illuminated once a day, and the chick embryos that died before 48 hours were discarded. After that, the embryos were illuminated once every 4-6 hours, and the dead chicken embryos were taken out at any time until 96 hours, regardless of whether they were dead or not, all were taken out, the air chamber was upright, and placed at 2-8°C for cooling for 12-24 hours.

[0033] 3 Harvest Take out the cooled chicken embryos, and harvest the chicken embryo allantoic fluid according to the requirements of the "...

Example Embodiment

[0040] Example 3: Preparation of vaccine

[0041] 1. Preparation of oil phase Take 95 parts of mineral oil and 1 part of aluminum stearate, mix them evenly in the oil phase preparation tank and heat to 80°C, add 5 parts of Siben-80, and keep it for 40 minutes when the temperature reaches 115°C , set aside after cooling.

[0042] 2. Aqueous phase preparation Mix the inactivated H9 subtype avian influenza virus venom in a ratio of 1:2. Take 95 parts of the mixed antigen solution and 5 parts of sterilized Tween-80, and mix thoroughly to dissolve the Tween-80 completely.

[0043] 3. Emulsification Take 2 parts of the oil phase and put it into the emulsification tank, start the motor, turn and stir at a slow speed, and add 1 part of the water phase slowly at the same time, and then stir at 3500r / min for 30-40 minutes. After emulsification, add 10ml of vaccine to a centrifuge tube, centrifuge at 3000r / min for 15 minutes, and the water precipitated at the bottom of the tube should ...

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Abstract

The invention aims to provide an avian influenza virus strain. The avian influenza virus strain has a preservation number of CCTCC No.V201617. The new H9 subtype avian influenza virus strain FJ 11 provided by the invention is high in titer of HA and EID<50> (egg infectious dose), good in immunogenicity, and can withstand attacks of local epidemic isolated virus of the H9 subtype avian influenza virus. The prepared vaccine is good in safety, and is free of any local or systemic adverse reactions caused by the vaccine. In the preservation period test, analysis of data of shape and properties, a safety test and a potency test is performed, the results show that all indexes are stable and effective compared with similar products.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to an avian influenza virus strain. Background technique [0002] Avian influenza is one of the severe infectious diseases of poultry. It is caused by type A influenza virus. When poultry is infected with H9 subtype avian influenza virus, especially when it is accompanied by mixed infection, it can lead to high mortality and a serious drop in egg production rate. Moreover, it is an acute and highly contagious disease with high mortality and high mortality, which is mainly popular in animals and humans. It occurs widely in all parts of the world and is very harmful to humans and the poultry industry. [0003] The variation of avian influenza virus among poultry epidemic viruses is relatively the most frequent, so that although a variety of vaccines have been introduced, the development of the epidemic is still uncontrollable, so it is necessary to s...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K39/145A61P31/16
CPCA61K39/12A61K2039/5252A61K2039/552A61K2039/55566C12N7/00C12N2760/16021C12N2760/16034
Inventor 李陆梅宋新宇马爽宫晓刘东郑欢郭伟伟
Owner YEBIO BIOENG OF QINGDAO
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