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Preparation method of polypeptide-gold nanoparticle-based metal ion detection biosensor

A gold nanoparticle, biosensor technology, applied in instruments, measuring devices, scientific instruments, etc., can solve problems such as the influence of detection conditions, and achieve uniform distribution, high coverage, and good selectivity.

Inactive Publication Date: 2016-11-09
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods have high sensitivity and good selectivity, but these methods are easily affected by detection conditions, and they still face great challenges in the analysis of actual samples.

Method used

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  • Preparation method of polypeptide-gold nanoparticle-based metal ion detection biosensor
  • Preparation method of polypeptide-gold nanoparticle-based metal ion detection biosensor
  • Preparation method of polypeptide-gold nanoparticle-based metal ion detection biosensor

Examples

Experimental program
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Embodiment 1

[0034] On Si / SiO by magnetic sputtering method 2 Deposit the gold interdigitated electrode on the surface, clean it with acetone and water in sequence, then treat it with a mixed solution of concentrated sulfuric acid: hydrogen peroxide (3:1) for 30 minutes, clean it with ionized water, and dry it with nitrogen.

[0035] Place the gold interdigitated electrode in 3-aminopropyltriethoxysilane for 15 minutes, take it out and quickly put it in a large amount of deionized water to clean it, and dry it with nitrogen.

[0036] Prepare a 40 μg / mL graphene oxide solution uniformly dispersed by ultrasound, take 20 μL and drop it on the interdigital electrode for 60 minutes, wash it with water, and dry it in the air. Using the interdigitated electrode as the working electrode, Ag / Cl as the reference electrode, and platinum electrode as the counter electrode, reduce in 0.1M PBS solution with a voltage of -1.2V for 10min.

[0037] Prepare 1mg / mL naphthalenemethylamine-N,N-dimethylformami...

Embodiment 2

[0040] On Si / SiO by magnetic sputtering method 2 Deposit the gold interdigitated electrode on the surface, clean it with acetone and water in sequence, then treat it with a mixed solution of concentrated sulfuric acid: hydrogen peroxide (3:1) for 30 minutes, clean it with ionized water, and dry it with nitrogen.

[0041] Place the gold interdigitated electrode in 3-aminopropyltriethoxysilane for 15 minutes, take it out and quickly put it in a large amount of deionized water to clean it, and dry it with nitrogen.

[0042] Prepare a 40 μg / mL graphene oxide solution uniformly dispersed by ultrasound, take 20 μL and drop it on the interdigital electrode for 60 minutes, wash it with water, and dry it in the air. Using the interdigitated electrode as the working electrode, Ag / Cl as the reference electrode, and platinum electrode as the counter electrode, reduce in 0.1M PBS solution with a voltage of -1.2V for 10min.

[0043] Prepare 1mg / mL naphthalenemethylamine-N,N-dimethylformami...

Embodiment 3

[0046] On Si / SiO by magnetic sputtering method 2 Deposit the gold interdigitated electrode on the surface, clean it with acetone and water in sequence, then treat it with a mixed solution of concentrated sulfuric acid: hydrogen peroxide (3:1) for 30 minutes, clean it with ionized water, and dry it with nitrogen.

[0047] Place the gold interdigitated electrode in 3-aminopropyltriethoxysilane for 15 minutes, take it out and quickly put it in a large amount of deionized water to clean it, and dry it with nitrogen.

[0048] Prepare a 40 μg / mL solution of carbon nanotubes uniformly dispersed by ultrasound, take 20 μL dropwise on the interdigitated electrode and keep for 60 min, wash it with water, and dry it in the air.

[0049] Prepare 1 mg / mL pyrenemethylamine-N, N-dimethylformamide solution, place the interdigital electrode in 1 mg / mL naphthylmethylamine-naphthylmethylamine solution for 30 minutes, and use N, N-dimethylformamide Rinse well and allow to dry.

[0050] Place the...

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Abstract

The invention provides a preparation method of a polypeptide-gold nanoparticle-based metal ion detection biosensor, and belongs to the technical field of biological sensing detection. The polypeptide-gold nanoparticle-based metal ion detection biosensor comprises a gold interdigital electrode and a graphene or carbon nano-tube channel, wherein gold nanoparticles and polypeptide molecules are connected on the channel surface, and the polypeptide molecules specifically recognize the metal ions to cause the electric conductance change of the channel so as to carry out metal ion detection. According to the present invention, the electrostatic effect is used to adsorb the gold nanoparticles onto the surface of the graphene, and the gold nanoparticles are uniformly distributed on the surface of the graphene and have the high density, such that the density of the polypeptide can be improved so as to improve the detection sensitivity; with the thiol group on the polypeptide molecule terminal group C, the polypeptide molecules can be easily immobilized on the surface of the gold nanoparticles, such that the coverage of the polypeptide on the surface of the gold nanoparticles is high; and the specially designed polypeptide molecules specifically recognize the target metal ions, such that the selectivity of the sensor on the target metal ion is high, and the sensor is suitable for the detection of the target metal ions in the complex water body.

Description

technical field [0001] The invention discloses a biosensor for detecting metal ions based on polypeptide-gold nanoparticles and a preparation method thereof, which is mainly used for highly sensitive and selective detection of metal ions in water bodies, and belongs to the technical field of biosensing detection. Background technique [0002] Heavy metal pollution in water not only destroys the balance of the ecosystem, but also poses a threat to human life and health. Therefore, it is of great significance to develop highly sensitive and rapid detection methods for (heavy) metal ions in water. Traditional metal ion detection methods mainly include atomic absorption method, inductively coupled plasma emission spectrometry, inductively coupled plasma mass spectrometry, ultraviolet spectrophotometry, and electrochemical analysis. However, these methods require more complicated pretreatment process, expensive equipment (such as inductively coupled plasma emission spectrometer, ...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/327
Inventor 谭峰王艺丛龙超姜晓
Owner DALIAN UNIV OF TECH
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