Phellochin F compound extracted from phellodendron amurense, phellodendron chinense and phellodendron chinense and application of phellochin F compound

The technology of Phellodendron Phellodendri and Gansuane is applied in the field of PhellochinF compound of Gansuane-type triterpene and its preparation, achieving the effect of important development and application prospects

Inactive Publication Date: 2016-11-16
ANSHUN PEOPLES HOSPITAL +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The base source of its medicinal materials is the bark of Phellodendron amurense or Phellodendron japonica, which is a plant of Rutaceae. It is mainly used to treat: damp-heat dysentery, diarrhea, jaundice; Pain; Carbuncle sore virus; Skin eczema, so far, in the prior art, there is no research report on the extraction of glycosane type triterpene Phellochin F and its activity from Cortex Phellodendri fruit

Method used

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  • Phellochin F compound extracted from phellodendron amurense, phellodendron chinense and phellodendron chinense and application of phellochin F compound
  • Phellochin F compound extracted from phellodendron amurense, phellodendron chinense and phellodendron chinense and application of phellochin F compound
  • Phellochin F compound extracted from phellodendron amurense, phellodendron chinense and phellodendron chinense and application of phellochin F compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043]Take 15 Kg of Phellodendron Phellodendron produced in Anshun, Guizhou, reflux and extract it with 95% industrial ethanol at a temperature of 80°C for 3 times, then recover and concentrate the ethanol to obtain 2.0 Kg of extract, which is miscible with water to form turbidity, and passed through ethyl acetate Equal volume extraction and concentration of the ethyl acetate layer yielded an extract of 0.9 Kg. The ethyl acetate layer was chromatographed on a 300-mesh silica gel column and eluted with a gradient eluent with a volume ratio of petroleum ether and acetone of 60:1. The thin-layer plate is combined into 4 parts: Fr 1, Fr 2, Fr 3, Fr 4, and the obtained 55.0g Fr 2 is subjected to silica gel column chromatography again, and the eluent is that the volume ratio of sherwood oil and ethyl acetate is 40: 1 Gradient elution, again divided into 4 sub-parts: Fr 2a, Fr 2b, Fr 2c, Fr 2d; for the four sub-parts, Fr 2d once again passed through the mobile phase as a mixture of ch...

Embodiment 2

[0049] Ampoule: Phellochin F 2 mg of the compound obtained in Example 1, 10 mg of sodium chloride;

[0050] Preparation method: Dissolve the compound and sodium chloride in an appropriate amount of water for injection, filter the resulting solution, and fill it into an ampoule bottle under sterile conditions.

Embodiment 3

[0052] Capsules: Phellochin F obtained in Example 1 10 mg, lactose 187 mg, magnesium stearate 3 mg;

[0053] Preparation method: mix Phellochin F with auxiliary agents, sieve, mix evenly, put the obtained mixture into hard gelatin capsules, each capsule weighs 200 mg, and the active ingredient is 10 mg.

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Abstract

The invention discloses a phellochin F compound extracted from phellodendron amurense, phellodendron chinense and phellodendron chinense and an application of the phellochin F compound. The compound is prepared from separating and extracting dried fruits of the phellodendron amurense, the phellodendron chinense and the phellodendron chinense which are produced in Anshun city of Guizhou, and the structural formula is as shown in a formula (I). A preparation method of the phellochin F compound comprises the following steps of extracting ethanol, concentrating a medicinal extract, performing silica gel column chromatography, performing gradient elution, performing sephadex column chromatograph, performing semi-preparation of high performance liquid chromatogram purification and the like. The Phellochin F compound disclosed by the invention is firstly extracted and separated from dried fruits of the phellodendron amurense, the phellodendron chinense and the phellodendron chinense which are produced in Anshun city of Guizhou, and the structural formula is determined. The compound has a treatment effect on cells with breast cancer cell line (MDA) of human bodies, and can induce the apoptosis of cells with the breast cancer cell line (MDA) of the human bodies. The phellochin F compound can be applied in medicines for treating breast cancer. The compound can be used as a lead compound of the medicines for treating the breast cancer.

Description

technical field [0001] The invention belongs to the field of medicines, and in particular relates to a triterpenoid Phellochin F compound extracted from Phellodendron sichuanensis fruit, a preparation method and application thereof. Background technique [0002] Gansuane-type triterpene Phellochin F is a class of triterpene compounds with a tetracyclic ring system. It is relatively rare in plant distribution and is a class of triterpenoids with a small amount. Published "Research Progress of Gansuane-type Triterpenes" in the journal "Chinese Herbal Medicine" and proposed that Gansuane-type triterpenes are precursors of a class of limonoids. Pharmacological studies have shown that this type of compound has certain activity on KB cells , but only weakly or inactively active against other types of tumor cells. This paper reviews the plant distribution and chemical structure classification of triterpenoids of glycosides. The main plant distributions are: Rutaceae, Bitterwood, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J9/00A61K31/575A61P35/00
CPCC07J9/00
Inventor 晏晨骆衡张云东王星慧黄丽荣王天元孙茂
Owner ANSHUN PEOPLES HOSPITAL
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