Application of oncostatin M in preparation of products for promoting mesenchymal stem cells to be in vitro directionally differentiated into bone cells

A technology of stem cells and oncostatin, which is applied in the field of cell biotechnology and bone tissue engineering, can solve problems such as low efficiency, and achieve the effects of stable biological properties, convenient material acquisition, and easy collection.

Inactive Publication Date: 2016-11-16
吉林省中科生物工程股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the efficiency of inducing osteogenic differ

Method used

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  • Application of oncostatin M in preparation of products for promoting mesenchymal stem cells to be in vitro directionally differentiated into bone cells
  • Application of oncostatin M in preparation of products for promoting mesenchymal stem cells to be in vitro directionally differentiated into bone cells
  • Application of oncostatin M in preparation of products for promoting mesenchymal stem cells to be in vitro directionally differentiated into bone cells

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Experimental program
Comparison scheme
Effect test

Embodiment 3

[0070] The preparation of embodiment 3 oncostatin M low dosage group (OSM-Low)

[0071] The umbilical cord mesenchymal stem cells were divided into 2×10 4 cells / cm 2 Inoculate in a 6-well culture plate at a density, and then place the culture plate in DMEM / F12 culture solution containing 10% fetal calf serum, osteogenic differentiation chemical inducer and 0.1ng / ml Oncostatin M for 7 to 21 sky.

[0072] The aforementioned chemical inducers of osteogenic differentiation include: 100 nmol / L of dexamethasone, 10 mmol / L of sodium β-glycerophosphate, and 50 mg / L of ascorbic acid diphosphate.

Embodiment 4

[0073] Preparation of Oncostatin M middle dose group (OSM-Mid) in embodiment 4

[0074] The umbilical cord mesenchymal stem cells were divided into 2×10 4 cells / cm 2 Density inoculated in 6-well culture plate, and then the culture plate was placed in culture solution containing 10% fetal calf serum, osteogenic differentiation chemical inducer and 1ng / ml DMEM / F12 for 7 to 21 days.

[0075] The aforementioned chemical inducers of osteogenic differentiation include: 100 nmol / L of dexamethasone, 10 mmol / L of sodium β-glycerophosphate, and 50 mg / L of ascorbic acid diphosphate.

Embodiment 5

[0076] The preparation of embodiment 5 Oncostatin M high-dose group (OSM-High)

[0077] The umbilical cord mesenchymal stem cells were divided into 2×10 4 cells / cm 2 Density inoculated in 6-well culture plate, and then the culture plate was placed in culture solution containing 10% fetal bovine serum, osteogenic differentiation chemical inducer and 10ng / ml DMEM / F12 for 7 to 21 days.

[0078] The aforementioned chemical inducers of osteogenic differentiation include: 100 nmol / L of dexamethasone, 10 mmol / L of sodium β-glycerophosphate, and 50 mg / L of ascorbic acid diphosphate.

[0079] Alkaline phosphatase assay

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Abstract

The invention belongs to the technical field of cell biotechnology and bone tissue engineering, and particularly relates to application of oncostatin M in preparation of products for promoting mesenchymal stem cells to be in vitro directionally differentiated into bone cells. Experiments prove that oncostatin M has functions of increasing expression of formed bone differentiation markers ALP, Runx2, OPN and OCN in mRNA and protein level and promoting the mesenchymal stem cells to be differentiated directionally into the bone cells. Application studying of oncostatin M matched with the mesenchymal stem cells in preparation of drugs for treating bone injury provides a material basis for development and preparation of drugs for treating diseases like osteoporosis, fracture bone loss and bone trauma.

Description

technical field [0001] The invention belongs to the technical fields of cell biotechnology and bone tissue engineering, and specifically relates to the application of oncostatin M in the preparation of products for promoting the directional differentiation of mesenchymal stem cells into bone cells in vitro. Background technique [0002] At present, bone defects and fracture nonunions caused by trauma, tumor, infection or improper fracture treatment are very common in clinic. Bone grafting (bone grafting) is an essential means for the treatment of bone defects and nonunion fractures. [0003] Traditional bone graft materials are mainly autologous bone, allogeneic bone and artificial bone material. Autologous bone is an ideal graft, which has rapid bone formation and no immune rejection, but its source is limited and often not enough to repair large bone defects, and the harvest will increase surgical trauma and may cause complications. The allogeneic bone provides a large a...

Claims

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Application Information

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IPC IPC(8): C12N5/077C12N5/0775A61L27/38
CPCC12N5/0654A61L27/3834A61L27/3847A61L27/3895C12N5/0665C12N2501/237C12N2506/1369C12N2509/00
Inventor 孔宁李云华孙飞
Owner 吉林省中科生物工程股份有限公司
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