Fowl adenovirus strain, inactivated vaccine and preparation method

Abstract

The invention provides a fowl adenovirus strain, an inactivated vaccine and a preparation method. The fowl adenovirus strain is named a fowl adenovirus TJ strain with a preservation number of CCTCC (China Center For Type Culture Collection) NO:V201632; the adenovirus strain is of race C in a fowl adenovirus I subgroup, the serotype is 4 type, viral nucleic acid is double stranded DNA (Deoxyribonucleic Acid), and no envelope is found; the diameter of virus particles is 70 to 90nm, and the virus particles are of icosahedral symmetrical structures; the inactivated vaccine is prepared by performing cell inoculation, venom harvesting, inactivating and emulsifying on a fowl adenovirus; a vaccine strain is a prevalent strain, and is good in immunogenicity and high in multiplication capacity; the prepared vaccine has the advantages of high safety, good stability, quick production of immune response, high virus challenge protecting rate, and the like.

Description

technical field [0001] The invention relates to a virus strain and an inactivated vaccine, in particular to a poultry adenovirus strain, an inactivated vaccine and a preparation method, and belongs to the technical field of bioengineering. Background technique [0002] Chicken inclusion body hepatitis-hydropericardium syndrome (Inclusion Body Heptatis-Hydroperioadium Syndrome, IBH-HPS) is a newly popular, acute, highly contagious chicken infectious disease, and its pathogen is Fowl adenovirus (FADV) , in the virus classification is Adenoviridae, subgroup I avian adenovirus in the genus Avian Adenovirus. The virus mainly infects 1-3 week-old broiler chickens, 817 hybrid broiler chickens, and Ma chickens, and is also more common in broiler breeders and laying hens, among which 3-7 week-old broiler chickens are more common. [0003] In China, the disease has become a nationwide epidemic, mainly occurring in Shandong, Henan, Anhui, Liaoning, Jilin, Hebei, Jiangsu and other plac...

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Problems solved by technology

[0004] In terms of diagnosis, the current diagnosis is mainly based on clinical symptoms, histopathological changes, PCR amplification, and virus isolation; in terms of disease prevention and control, although there have been research reports on the use of chicken embryos and poultry-derived cells to culture avian adenoviruses, However, the serotypes of most of the reported strains are inconsistent with the current market strains. Therefore, the current market urgently needs to develop a poultry gland with a serotype consistent with the current market strains, high titer, and good immunogenicity. virus vaccine