Fowl adenovirus strain, inactivated vaccine and preparation method

A technology of avian adenovirus and inactivated vaccine, which is applied in the field of bioengineering to achieve the effects of high safety, high protection rate against virus and rapid immune response

Active Publication Date: 2016-11-16
RINGPU (BAODING) BIOLOGICAL PHARMACEUTICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In terms of diagnosis, the current diagnosis is mainly based on clinical symptoms, histopathological changes, PCR amplification, and virus isolation; in terms of disease prevention and control, although there have been research reports on the use of chicken embryos and poultry-derived cells to culture

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1 uses chicken embryo kidney cell (CEK cell) to prepare poultry adenovirus inactivated vaccine

[0040] The formula of the cell growth solution is: M199 solution with a volume percentage of 90%, 10% newborn bovine serum, and adjust the pH value to 7.2;

[0041] The formula of the cell maintenance solution is: M199 solution with a volume percentage of 98%, 2% newborn bovine serum, 2.4mmol / L arginine, and adjust the pH value to 7.4;

[0042] a. Preparation of CEK cells: Take 17-day-old SPF chicken embryos, take out the kidneys in a sterile environment, place them in an appropriate amount of trypsin solution, and digest them at 37°C. After the digestion is complete, take the supernatant and add an appropriate amount of serum to stop the digestion, centrifuge at 1500rpm for 10min, discard the supernatant, resuspend the cell pellet with cell growth medium and dilute to a final concentration of 1×10 cells. 6 Cells / ml, cultured at 37°C for 72 hours, the cells grow ...

Embodiment 2

[0053] Embodiment 2 uses chicken embryo fibroblast cell line (DF1 cell) to prepare poultry adenovirus inactivated vaccine

[0054] The formula of growth medium for cells is: DMEM / F12 solution with a volume percentage of 92%, 8% newborn bovine serum, and adjust the pH value to 7.2;

[0055] The formula of the cell maintenance solution is: 97% DMEM / F12 solution by volume, 3% newborn bovine serum, 2.2mmol / L arginine, and adjust the pH value to 7.3;

[0056] a. Cell culture:

[0057] The DF1 seed cells were removed from the liquid nitrogen and melted in a 37°C water bath, and revived with cell growth medium (DMEM / F12 medium) for culture. After 3 days of culture, the number of cells was 4.0×10 7.0 Cells / ml, and passaged according to 1:3, cultured at 37°C for 2 days;

[0058] b. Virus inoculation: avian adenovirus by 10 3.0 ELD 50 Inoculate DF1 cells per 0.1ml, add cell maintenance solution (DMEM / F12 medium), and continue culturing at 37°C;

[0059] c. Harvesting venom: When th...

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PUM

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Abstract

The invention provides a fowl adenovirus strain, an inactivated vaccine and a preparation method. The fowl adenovirus strain is named a fowl adenovirus TJ strain with a preservation number of CCTCC (China Center For Type Culture Collection) NO:V201632; the adenovirus strain is of race C in a fowl adenovirus I subgroup, the serotype is 4 type, viral nucleic acid is double stranded DNA (Deoxyribonucleic Acid), and no envelope is found; the diameter of virus particles is 70 to 90nm, and the virus particles are of icosahedral symmetrical structures; the inactivated vaccine is prepared by performing cell inoculation, venom harvesting, inactivating and emulsifying on a fowl adenovirus; a vaccine strain is a prevalent strain, and is good in immunogenicity and high in multiplication capacity; the prepared vaccine has the advantages of high safety, good stability, quick production of immune response, high virus challenge protecting rate, and the like.

Description

technical field [0001] The invention relates to a virus strain and an inactivated vaccine, in particular to a poultry adenovirus strain, an inactivated vaccine and a preparation method, and belongs to the technical field of bioengineering. Background technique [0002] Chicken inclusion body hepatitis-hydropericardium syndrome (Inclusion Body Heptatis-Hydroperioadium Syndrome, IBH-HPS) is a newly popular, acute, highly contagious chicken infectious disease, and its pathogen is Fowl adenovirus (FADV) , in the virus classification is Adenoviridae, subgroup I avian adenovirus in the genus Avian Adenovirus. The virus mainly infects 1-3 week-old broiler chickens, 817 hybrid broiler chickens, and Ma chickens, and is also more common in broiler breeders and laying hens, among which 3-7 week-old broiler chickens are more common. [0003] In China, the disease has become a nationwide epidemic, mainly occurring in Shandong, Henan, Anhui, Liaoning, Jilin, Hebei, Jiangsu and other plac...

Claims

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Application Information

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IPC IPC(8): C12N7/00A61K39/235A61P31/20
CPCA61K39/12A61K2039/5252A61K2039/552C12N7/00C12N2710/10234
Inventor 王帅韩佳丽郁宏伟刘涛何平有朱秀同杨保收梁武刘超
Owner RINGPU (BAODING) BIOLOGICAL PHARMACEUTICAL CO LTD
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