Receptor-modified and receptor-free titanium dioxide nano metal film Raman chips and manufacturing method

Abstract

The invention discloses receptor-modified and receptor-free titanium dioxide nano metal film Raman chips and a manufacturing method. Each chip is composed of a surface enhanced Raman scattering (SERS) film and a carrier thereof, the carriers comprise the capillary type carrier, the small box type carrier and the microfluidic channel type carrier, the SERS-films comprise the receptor-modified SERS-film and the receptor-free SERS-film, and six types of the Raman chips are obtained. Control standards are set in the manufacturing method, and the controllable SERS-film manufacturing method comprises the steps that 1, a titanium dioxide film is pulled through a sol-gel method, and scattered particles are crystallized; 2, a second-layer nano silver foil/silver particle SERS-film grows through photocatalysis; 3, the second-layer SERS-film is modified with specifically coordinated and combined receptor molecules through hydrogen bonding force or immune bonding force according to the needs of detected object donor molecules, and then a third-layer SERS-film is formed. The Raman chips can be applied to environmental pollution treatment and detection, hard drug and warfare agent detection, pesticide residue detection, harmful food additive screening, large-scale early-stage cancer Raman screening and the like.

Description

technical field [0001] The invention relates to the field of Raman analysis chips in spectral analysis technology, in particular to a Raman chip with or without acceptor titanium dioxide nanometer metal film and a manufacturing method. Background technique [0002] The surface near-field enhanced Raman spectroscopy (SERSp) analysis technology established by the principle of the surface near-field enhanced Raman scattering (SERS) effect is the most commonly used method of mixing nano-silver particles and liquid phase samples into the capillary. Raman sample cell; In addition, a variety of Raman sample cell technical solutions have been disclosed, for example: first build TiO on the inner wall of the capillary 2 Nanotube arrays, silver nanoparticles modified on TiO by Sn ion coupling 2 Capillary sample cell on the surface of the tube array "CN102706857A, a method for preparing a multifunctional surface-enhanced Raman scattering substrate"; three-dimensional porous and ordered...

AI Technical Summary

Problems solved by technology

[0009] At present, almost all high-sensitivity SERS Raman active substrates have the problems of poor repeatability, consistency and stability of the analysis results, and the error is too large for absolute quantitative analysis. The problem of no results; the fundamental reason is that the production of the silver particle film requires very fine and harsh requirements - the size of the silver particle is required to be resonantly coupled with the wavelength of the exciting laser beam, and the silver particles must be close together, with a distance of ≤2nm, which is difficult to obtain. It is even more difficult to do it, and it is even more difficult to require each silver particle film product to meet the same standard; if the average spacing of silver particles is greater than 4 nanometers, the Raman signal of SERSp will be an order of magnitude lower; therefore, in the SERS silver particle film If there are no strict control methods and control standards in the manufacturing process, it will not be possible to solve the problems of poor repeatability, consistency and stability of SERSp analysis results

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