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Qualitative detection method for content of beta-receptor stimulants in milk

A receptor agonist, qualitative detection technology, applied in the direction of biological testing, material inspection products, etc., can solve the problem that the content of β-receptor agonist needs to be improved

Active Publication Date: 2016-11-23
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the current detection methods for the content of β-receptor agonists still need to be improved

Method used

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  • Qualitative detection method for content of beta-receptor stimulants in milk
  • Qualitative detection method for content of beta-receptor stimulants in milk
  • Qualitative detection method for content of beta-receptor stimulants in milk

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Reagents and solutions.

[0023] The β-receptor agonist detection kit was purchased from EP Company in the Netherlands.

[0024] The substrate: reconstituted milk obtained by dissolving whole milk powder purchased from New Zealand Fonterra Company in water at a mass ratio of 1:7.

[0025] Positive control: reconstituted milk with known content of β-agonist.

[0026] 2. Equipment and instruments.

[0027] BioTek company's ELx808 microplate reader, detection wavelength: 450nm.

[0028] Waters premier ultra-high performance liquid chromatography tandem mass spectrometer.

[0029] 3. Operation steps.

[0030] (1) Take out the required number of microporous tubes and insert them into the microporous plate, and record the positions of the microwells;

[0031] (2) Centrifuge the substrate, the positive quality control and the milk to be tested at a temperature of 2-8°C and a speed of 3000g for 10 minutes, collect the supernatant, and add the supernatant to the micropor...

Embodiment 2

[0043] accuracy check

[0044] Take 4 kinds of milk samples that do not contain β-receptor agonists, add different concentrations of β-receptor agonist standard products (commercially available) therein according to the concentrations shown in the table below to obtain spiked milk, and use the The method of Example 1 and GB / T22965-2008 "The Determination of 12 Kinds of Beta-Stimulant Residues in Milk and Milk Powder by Liquid Chromatography-Tandem Mass Spectrometry" (hereinafter referred to as chromatography) carried out 2 parallel detections on different spiked milks, The substrate is reconstituted milk without β-receptor agonist, and the positive quality control product is reconstituted milk with 0.15ng / mL β-receptor agonist added.

[0045] The results are shown in Table 1. Add β-receptor agonist standard substances to the four milk samples respectively. When the added concentration is lower than 0.15ng / mL, the absorbance value is higher than that of the positive quality co...

Embodiment 3

[0051] False Positive Rate Detection

[0052] Take 4 kinds of milk samples that do not contain β-receptor agonists, add different concentrations of β-receptor agonist standard products (commercially available) therein according to the concentrations shown in the table below to obtain spiked milk, and use the The method of Example 1, enzyme-linked immunosorbent assay kit and chromatography were used to perform two parallel detections on different spiked milks. The substrate was reconstituted milk without adding β-receptor agonists, and the positive quality control was added with 0.15ng / mL β-agonist reconstituted milk.

[0053] The detection process of the ELISA quantitative kit is as follows:

[0054] (1) Determination of the OD value of the sample to be tested according to steps (1) to (8) of Example 1, wherein step (1) does not need to set the substrate and positive quality control;

[0055] (2) According to the steps (1) to (8) of Example 1, measure the OD values ​​of dif...

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Abstract

The invention discloses a qualitative detection method for the content of beta-receptor stimulants in milk. The qualitative detection method includes the steps that 1, a substrate, a positive quality control product and to-be-detected milk are detected respectively with the enzyme-linked immunoassay method, the OD value of the substrate, the OD value of the positive quality control product and the OD valued of the to-be-detected milk are obtained, wherein the positive quality control product is reconstituted milk containing the known-content beta-receptor stimulants, the substrate is reconstituted milk which is free of beta-receptor stimulants, and the reconstituted milk is obtained in the mode that whole milk powder purchased from New Zealand is dissolved in water; 2, based on the detection result, the to-be-detected milk is subjected to qualitative detection. The qualitative detection method is easy and convenient to operate and high in accuracy.

Description

technical field [0001] The invention relates to the field of food. Specifically, the present invention relates to a qualitative detection method for β-receptor agonist content in milk. Background technique [0002] β-receptor agonists are mostly formed by linking phenylethanolamine with tert-butyl or isopropyl. According to the different substituents on the benzene ring in the core structure, they are divided into three types: aniline type, phenol type and resorcinol type. Representative compounds include clenbuterol, salbutamol, etc. β-receptor agonists can promote fat decomposition in animals, increase protein synthesis, and increase the lean meat rate of ketone bodies, but large doses are likely to remain in edible animal tissues. After humans eat tissues with drug residues, they will cause poisoning symptoms such as increased breathing, headache, nausea and muscle tremors, which can induce and aggravate the condition of patients with cardiovascular and cerebrovascular ...

Claims

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Application Information

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IPC IPC(8): G01N33/74
CPCG01N33/74
Inventor 解鑫喻东威赵媛梁春梅许璐袁凤琴任丽刘美霞李秋琴宋晓东
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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