Heat-stability mannosylerythritol lipid carrier and preparing method and application thereof

A heat-stable, sugar-erythritol technology, applied in the field of heat-stable mannose erythritol lipid carrier and its preparation and application, can solve the problems of insoluble molecules, unstable carrier and hydrophobic food, etc., and the method is simple Feasible, low cost, good embedding effect

Inactive Publication Date: 2016-12-07
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The invention provides a heat-stable mannose erythritol lipid carrier, which has thermodynamic stability an

Method used

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  • Heat-stability mannosylerythritol lipid carrier and preparing method and application thereof
  • Heat-stability mannosylerythritol lipid carrier and preparing method and application thereof
  • Heat-stability mannosylerythritol lipid carrier and preparing method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0029] The hydrophobic substance embedded in this embodiment is betulinic acid.

[0030] (1) Preparation of mannose erythritol lipid carrier

[0031] Dissolve MEL-A and DLPC in chloroform to prepare solutions with a concentration of 1.0 mg / L, mix the two solutions in a test tube at a volume ratio of 5:5, and let stand for 2 to 3 weeks until the chloroform evaporates completely. Add 2.0 mL of deionized water to the test tubes, vortex and mix well, ultrasonicate for 5 min, and let stand to prepare the mannose erythritol lipid carrier solution.

[0032] (2) Evaluation of properties of mannose erythritol lipid carrier

[0033] The particle size of the system was measured by a nanometer particle size analyzer to be 376.4nm. The thermodynamic stability of the solution system was determined by differential scanning calorimetry.

[0034] (3) Betulinic acid embedded in mannose erythritol lipid carrier

[0035] Take the MEL / DLPC carrier solution, add betulinic acid with a mass perce...

Embodiment 2

[0039] The hydrophobic substance embedded in this embodiment is α-linolenic acid.

[0040] (1) Preparation of mannose erythritol lipid carrier

[0041] Dissolve MEL-A and DLPC in chloroform to prepare solutions with a concentration of 1.0 mg / L, mix the two solutions in a test tube at a volume ratio of 7:3, and let stand for 2 to 3 weeks until the chloroform evaporates completely. Add 2.0 mL of deionized water to the test tubes, vortex and mix well, ultrasonicate for 5 min, and let stand to prepare the mannose erythritol lipid carrier solution.

[0042] (2) Evaluation of properties of mannose erythritol lipid carrier

[0043]The particle size of the system was measured by a nanometer particle size analyzer to be 189.5 nm. The thermodynamic stability of the solution system was determined by differential scanning calorimetry.

[0044] (3) Mannose erythritol lipid carrier embedding α-linolenic acid

[0045] Take the MEL / DLPC carrier solution, add α-linolenic acid with a concen...

Embodiment 3

[0049] The hydrophobic substance embedded in this embodiment is vitamin E.

[0050] (1) Preparation of mannose erythritol lipid carrier

[0051] Dissolve MEL-A and DLPC in chloroform to prepare solutions with a concentration of 1.0 mg / L, mix the two solutions in a test tube at a volume ratio of 7:3, and let stand for 2 to 3 weeks until the chloroform evaporates completely. Add 2.0 mL of deionized water to the test tubes, vortex and mix well, ultrasonicate for 5 min, and let stand to prepare the mannose erythritol lipid carrier solution.

[0052] (2) Evaluation of properties of mannose erythritol lipid carrier

[0053] The particle size of the system was measured by a nanometer particle size analyzer to be 189.5 nm. The thermodynamic stability of the solution system was determined by differential scanning calorimetry.

[0054] (3) Vitamin E embedded in mannose erythritol lipid carrier

[0055] Take the MEL / DLPC carrier solution, add vitamin E with a mass percentage concentr...

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Abstract

The invention discloses a heat-stable mannose erythritol lipid carrier, which is prepared by self-assembly of mannose erythritol lipid and lecithin in aqueous solution. The invention also provides the preparation method and application of the carrier. The carrier provided by the invention has thermodynamic stability, has better embedding effect on hydrophobic substances, and the materials used are safe and non-toxic, does not need other additives, and has simple and feasible preparation method and low cost.

Description

technical field [0001] The invention relates to a thermally stable mannose erythritol lipid carrier and a preparation method and application thereof, belonging to the field of biological materials and food additives. Background technique [0002] In recent years, special structures formed by amphiphilic molecules relying on self-assembly properties, such as micelles, vesicles, coacervates, etc., have received great attention in the fields of life sciences and materials. Among them, the lipid carrier is a closed vesicle with a bilayer structure formed by the aggregation of the hydrophobic tails of the amphiphilic molecules, while the hydrophilic head is exposed to the water phase. Lipid carriers can be used as carriers of water-soluble drugs or genes, and can also use the bilayer structure to carry oil-soluble substances (Imura, Yanagishita, Ohira, et al., 2005). However, its application is limited, mainly because most carriers formed by external energy, such as ultrasound, ...

Claims

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Application Information

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IPC IPC(8): A23P10/35
Inventor 陈启和范琳琳牛永武
Owner ZHEJIANG UNIV
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