Method for biosynthesis of hydroxybutyric acid and hydroxycaprylic acid co-polymer from sludge

A technology of hydroxyoctanoic acid and hydroxybutyric acid, applied in sludge treatment, biological sludge treatment, fermentation, etc., can solve the problems of unoptimistic economic benefits and low added value of products, and achieve the effect of resource recycling

Active Publication Date: 2016-12-07
SANMING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether activated sludge is used to produce methane or hydrogen, the added va...

Method used

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  • Method for biosynthesis of hydroxybutyric acid and hydroxycaprylic acid co-polymer from sludge
  • Method for biosynthesis of hydroxybutyric acid and hydroxycaprylic acid co-polymer from sludge

Examples

Experimental program
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Effect test

Embodiment 1

[0023] A method of sludge biosynthesis P (HB-HO), the method is:

[0024] (1) Each domestication cycle is 24 h, and the operation mode of each cycle is: water inflow + aerobic aeration + sludge discharge + sedimentation + water outflow; the inflow is simulated wastewater, and the inflow volume is 5 L; , the air compressor was used for aeration, and the aeration rate was kept at 2 L / min; a certain amount of activated sludge mixture was discharged before the aerobic aeration was stopped, so as to control the sludge concentration in the reactor to 2 g / L, Sludge concentration is calculated in MLSS; finally, the supernatant is discharged after sedimentation; the total time from stopping aerobic aeration to re-aeration does not exceed 20 minutes each time; samples are taken to measure MLSS, SVI, and pH before sludge discharge in each cycle When the above values ​​are stable and the sludge color is yellow-brown, the sludge domestication is completed; about 2g / L, the SVI value is abou...

Embodiment 2

[0028] Temperature and pH were not controlled during acclimatization. Each domestication cycle is 12 hours, and the operation mode of each cycle is: water inflow + aerobic aeration + mud discharge + sedimentation + water outflow. The influent is simulated wastewater, and the influent volume is 4L; after the influent, the aeration pump is used for aeration, and the aeration rate is kept at 1.0 L / min; a certain amount of activated sludge is discharged before the aerobic aeration is stopped. liquid, to control the sludge concentration in the reactor to 1 g / L, and the sludge concentration is calculated as the suspended solids concentration of the mixed liquor; finally, the supernatant is discharged after precipitation. It is advisable that the total time from stopping aerobic aeration to re-aeration should not exceed 30 minutes each time. Sampling and measuring indicators such as MLSS and SVI are taken before sludge discharge in each cycle. When the above values ​​are stable and ...

Embodiment 3

[0032](1) Each domestication cycle is 24 hours, and the operation mode of each cycle is: water inflow + aerobic aeration + sludge discharge + sedimentation + water outflow; the inflow is simulated wastewater, and the inflow volume is 5 L; , the air compressor was used for aeration, and the aeration rate was kept at 2 L / min; a certain amount of activated sludge mixture was discharged before the aerobic aeration was stopped, so as to control the sludge concentration in the reactor to 2 g / L, Sludge concentration is calculated in MLSS; finally, 5 L of supernatant is discharged after sedimentation; the total time from stopping aerobic aeration to re-aeration does not exceed 30 min each time; samples are taken to measure MLSS and SVI before sludge discharge in each cycle , pH value and DO, when the above values ​​are stable, when the sludge color is yellowish brown, the sludge domestication is completed; about 2g / L, the SVI value is about 65, and the sludge age is 5d.

[0033] (2) A...

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Abstract

The invention provides a method for biosynthesis of a hydroxybutyric acid and hydroxycaprylic acid co-polymer from sludge. Aerobic domestication is performed on the activated sludge with sodium laurate used as a carbon source. Each domestication period is 12-36 h and adopts the running manner of water inlet, aerobic aeration, sludge discharging, sedimentation and water outlet. Preferably, the total time from stopping of aerobic aeration to re-aeration each time does not exceed 30 min. In each period, sampling is performed before sludge discharging, MLSS (mixed liquor suspended solids), the pH value and DO (dissolved oxygen) are tested, and after the values are stabilized, sludge domestication is completed. After sludge domestication is stabilized, the next step of a test for synthesis of the hydroxybutyric acid and hydroxycaprylic acid co-polymer from the sludge can be conducted. Production of a biochemical product with the higher added value from a cheap substrate, namely, the activated sludge, can be a new way for recycling of the sludge.

Description

technical field [0001] The invention belongs to the field of sludge treatment, and in particular relates to a method for biologically synthesizing hydroxybutyric acid and hydroxyoctanoic acid copolymers from sludge. Background technique [0002] The activated sludge produced in the sewage treatment process is the aggregate of microorganisms and organic matter naturally formed in the sewage treatment system, containing a large amount of carbohydrates, lipids, proteins, vitamins and other organic matter and different types of microorganisms. After treatment (such as hydrolytic acidification), it can be converted into small molecular substances such as butyric acid, propionic acid, and acetic acid, and become a good substrate for the synthesis of PHA, and the mixture of small molecular fatty acids such as acetic acid, propionic acid, butyric acid, and valeric acid can be The copolymer of 3-hydroxybutyric acid (HB) and 3-hydroxyvaleric acid (HV) is obtained. PHVB is one of the m...

Claims

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Application Information

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IPC IPC(8): C02F11/02C08G63/06C08G63/78C12P7/62
CPCC02F11/02C08G63/06C08G63/78C12P7/625Y02W10/20
Inventor 董国文王仁章李奇勇张丽华牛玉
Owner SANMING UNIV
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