Hyperuricemia cell model, building method thereof and application of model to uric acid reduction efficacy evaluation

A hyperuricemia and cell model technology, applied in the field of cell model construction, can solve the problems of long cycle and cumbersome drug screening experiments, and achieve the effect of short cycle and simple operation
CN106190953AActive Publication Date: 2016-12-07SOUTH CHINA UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTH CHINA UNIV OF TECH
Publication Date
2016-12-07

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Abstract

The invention relates to a hyperuricemia cell model, a building method thereof and an application of the model to uric acid reduction efficacy evaluation. The building method includes the steps: (1) taking human proximal tubular epithelial cells (HK-2 cells) as model making cells, dissociating the HK-2 cells in a logarithmic phase to prepare cell suspension, adjusting cell density and culturing the cells in a cell culture box for 12-36 hours after planking; (2) removing cell supernatant, cleaning the cells with PBS (phosphate buffer solution) for 2-3 times, adding adenosine and continuing incubation for 12-36 hours; (3) adding xanthine oxidase into the cell supernatant, continuing culture for 12-36 hours, taking out the cell supernatant, and performing efficient liquid detection of uric acid content. Experimental results indicate that the hyperuricemia cell model can be obtained by the aid of the inductor adenosine and the xanthine oxidase. The hyperuricemia cell model is built for the first time, uric acid reduction efficacy of a medicine can be simply and rapidly analyzed, and the model is of great significance for gout medicine research.
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Description

technical field

[0001] The invention belongs to the technical field of cell model construction, and in particular relates to a method for constructing a hyperuricemia cell model and its application in the evaluation of uric acid-lowering efficacy. Background technique

[0002] Hyperuricemia is a metabolic disease caused by purine metabolism disorder and / or decreased uric acid excretion. It is mainly characterized by elevated blood uric acid and is an important biochemical basis for gout, kidney stones and other diseases. In recent years, the prevalence of hyperuricemia and gout has been increasing, and hyperuricemia, as one of many metabolic disorders, is often closely related to the development of hypertension, diabetes, obesity, atherosclerosis and other diseases , is an important disease threatening human beings.

[0003] The current drugs for the treatment of hyperuricemia can be divided into: drugs that inhibit the production of uric acid; drugs that promote the excret...

Claims

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