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Culture medium for producing abamectin by means of fermentation by aid of streptomyces griseus

A technology of streptomyces griseus and abamectin, applied in the field of fermentation, can solve the problems of weak production capacity, long fermentation cycle, high energy consumption, etc., to promote the growth and metabolism of mycelia, improve the vitality of strains, and increase the fermentation unit Effect

Inactive Publication Date: 2016-12-07
NINGXIA TAIYICIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the early stage of fermentation of abamectin, the initial potency is low, and in the late stage of fermentation, the ability of producing hormones is weak, and the potency increase is small, which affects the overall level of fermentation potency
[0005] 2 The fermentation cycle is long, generally around 240h, resulting in higher energy consumption and increased production costs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Primary seed culture: the effective volume of the seed medium is 2m 3 .

[0032] Seed medium: 60kg of corn starch, 16kg of soybean cake powder, 70kg of peanut cake powder, 8kg of yeast extract, 0.06 kg of cobalt chloride, and 0.08kg of α-amylase.

[0033] First, sterilize the primary seed medium at 121°C, hold the pressure for 30 minutes, cool to 25°C, and keep the pressure with sterile air. The quality requirements of the primary seed medium after sterilization are: amino nitrogen 42mg / 100ml, total Sugar 2.8g / 100ml, pH7.2. Then, under the protection of the flame, the cultured Streptomycin griseus mother bottle fermentation liquid was inserted into the first-level seed medium according to the inoculum amount of 0.1L for seed culture. The first-level seed culture conditions are: tank pressure 0.03-0.05MPa ; Tank temperature 28±0.5℃; Air flow 180m 3 / h; stirring speed 160r / min. Inoculate until the cell concentration is 25.2%, the pH value is 6.6, and the incubation ti...

Embodiment 2

[0049] Primary seed culture: the effective volume of the seed medium is 2m 3 .

[0050]Seed medium: 60kg of corn starch, 16kg of soybean cake powder, 70kg of peanut cake powder, 8kg of yeast extract, 0.06 kg of cobalt chloride, and 0.08kg of α-amylase.

[0051] First, sterilize the primary seed medium at 121°C, hold the pressure for 30 minutes, cool to 25°C, and keep the pressure with sterile air. The quality requirements for the sterilized primary seed medium are: amino nitrogen 42mg / 100ml, Total sugar 2.8g / 100ml, pH7.2. Then, under the protection of the flame, the cultured Streptomycin griseus mother bottle fermentation liquid was inserted into the first-level seed medium according to the inoculum amount of 0.1L for seed culture. The first-level seed culture conditions are: tank pressure 0.03-0.05MPa ; Tank temperature 28±0.5℃; Air flow 180m 3 / h; stirring speed 160r / min. Transplant until the cell concentration is 24.6%, the pH value is 6.7, and the culture time is 31 ho...

Embodiment 3

[0067] Primary seed culture: the effective volume of the seed medium is 2m 3 .

[0068] Seed medium: 60kg of corn starch, 16kg of soybean cake powder, 70kg of peanut cake powder, 8kg of yeast extract, 0.06 kg of cobalt chloride, and 0.08kg of α-amylase.

[0069] First, sterilize the primary seed medium at 121°C, hold the pressure for 30 minutes, cool to 25°C, and keep the pressure with sterile air. The quality requirements for the sterilized primary seed medium are: amino nitrogen 42mg / 100ml, Total sugar 2.8g / 100ml, pH7.2. Then, under the protection of the flame, the cultured Streptomycin griseus mother bottle fermentation liquid was inserted into the first-level seed medium according to the inoculum amount of 0.1L for seed culture. The first-level seed culture conditions are: tank pressure 0.03-0.05MPa ; Tank temperature 28±0.5℃; Air flow 180m 3 / h; stirring speed 160r / min. Transplant until the cell concentration is 26.4%, the pH value is 6.9, and the culture time is 34 h...

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PUM

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Abstract

The invention relates to a culture medium for producing abamectin by means of fermentation by the aid of streptomyces griseus. The culture medium comprises secondary seed culture media and fermentation middle-late stage supplementary culture media. The culture medium has the advantages that substances such as fermented bean pulp, zinc lactate and soy peptones are added into the culture medium, accordingly, the culture medium is favorable for growth and metabolism of bacteria and increase of fermentation starting potency, the aging speeds of hyphae can be reduced, the abamectin productivity of the abamectin bacteria can be improved, the fermentation periods can be shortened, the fermentation cost can be lowered, and the abamectin can be efficiently produced.

Description

technical field [0001] The invention belongs to the technical field of fermentation, in particular to a culture medium for producing abamectin by fermenting Streptomyces griseus. Background technique [0002] Abamectin is a class of 16-membered macrolide compound with insecticidal, acaricidal and nematicidal activities first developed by Satoshi Omura of Kitasato University in Japan and Merck Company of the United States. It is fermented by Streptomyces avermitilis in Streptomyces griseus produce. Natural abamectin contains 8 components, mainly 4 kinds, namely A1a, A2a, B1a and B2a, the total content of which is ≥80%; the corresponding 4 homologues with smaller proportions are A1b, A2b, B1b and B2b , its total content ≤ 20%. Among them, B1a is the main insecticidal component, so the content of abamectin is calibrated by the content of B1a. Abamectin has the characteristics of agriculture, animal husbandry and medicine. It has strong insect repellent and insecticidal acti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/62C12R1/545
CPCC12P19/623C12N1/205C12R2001/545
Inventor 裴立忠张江飞郭佳王勇平于荣尤永福
Owner NINGXIA TAIYICIN BIOTECH CO LTD