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Kit for determining glycosylated hemoglobin and preparation method thereof

A glycated hemoglobin and kit technology, which are applied in the field of a glycated hemoglobin determination kit and its preparation, can solve the problems of complicated operation and low measurement accuracy, and achieve the advantages of improving sensitivity, avoiding insufficient binding and avoiding non-specific agglutination. Effect

Inactive Publication Date: 2016-12-07
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a kit for measuring glycosylated hemoglobin and its preparation method in order to overcome the defects of complicated operation and low measurement accuracy in the prior art

Method used

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  • Kit for determining glycosylated hemoglobin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] The test kit of the present invention comprises reagent R1, reagent R2 and reagent R3 three liquid components which are independent of each other, wherein

[0089] Reagent R1:

[0090] Latex glycine buffer 100 mmol / L

[0091] Polyethylene glycol-2000 15 g / L

[0092] Tween-80 3.0 ml / L

[0093] Glycerin 20 mmol / L

[0094] Bovine Serum Albumin 40 g / L

[0095] Sodium azide 0.6 g / L

[0096] The solvent is purified water

[0097] Reagent R2:

[0098] Glycine buffer 100 mmol / L

[0099] Anti-IgG antibody 10g / L

[0100] Anti-HbA1c antibody 10 g / L

[0101] Sodium chloride 200 mmol / L

[0102] Bovine serum albumin 20 g / L

[0103] Sodium azide 0.5 g / L

[0104] The solvent is purified water

[0105] Reagent R3:

[0106] Hemolysin diluent 50mmol / L

[0107] Its solvent is purified water.

Embodiment 2

[0109] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein

[0110] Reagent R1:

[0111] Latex glycine buffer 15mmol / L

[0112] Polyethylene glycol-2000 25 g / L

[0113] Tween-80 1.0 ml / L

[0114] Glycerin 30 mmol / L

[0115] Bovine serum albumin 20g / L

[0116] Sodium azide 0.9 g / L

[0117] The solvent is purified water

[0118] Reagent R2:

[0119] Glycine buffer 50 mmol / L

[0120] Anti-IgG antibody 16g / L

[0121] Anti-HbA1c antibody 8 g / L

[0122] Sodium chloride 250 mmol / L

[0123] Bovine Serum Albumin 10 g / L

[0124] Sodium azide 0.9 g / L

[0125] The solvent is purified water

[0126] Reagent R3:

[0127] Hemolysin diluent 55mmol / L

[0128] Its solvent is purified water.

Embodiment 3

[0130] Kit preparation and method of use

[0131] 1. Prepare latex glycine buffer:

[0132] (1) Weigh glycine and dissolve it in purified water, stir well, and prepare 100 mmol / L glycine buffer solution for later use;

[0133] (2) Select polystyrene microspheres with a particle size of 450nm, disperse the polystyrene microspheres with the glycine buffer solution prepared in step (1), and prepare the contained polystyrene microspheres at a mass concentration of 3% The solution;

[0134] (3) Add 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride to the solution prepared in the above step (2), wherein 0.5 mg of 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, react at 25°C for 2 hours, use a centrifuge, and centrifuge at 15000 rpm / min for 30 minutes , discard the supernatant, and then dissolve the precipitate in 100 mmol / L glycine buffer so that the mass concentration of the polystyrene microspheres contained is 3%, ultrasonically disperse, and then use a...

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Abstract

The invention discloses a kit for determining glycosylated hemoglobin and a preparation method thereof. The kit comprises three liquid components a reagent R1, reagent R2 and a reagent R3 that are independently to each other. The reagent R1 consists of: a latex glycine buffer solution, polyethylene glycol-2000, Tween-80, glycerin, bovine serum albumin, and sodium azide, the reagent R2 consists of: a glycine buffer solution, an anti-IgG antibody, an anti-HbA1c antibody, sodium chloride, bovine serum albumin and sodium azide, and the reagent R3 consists of: a hemolysin diluent. The preparation method includes: preparing the reagents according to the component content; mixing a to-be-determined sample with the reagent 3, and using the hemolysin diluents to treat the to-be-determined sample; mixing the treated to-be-determined sample with the reagent R1 and reagent R2 and carry out full reaction; employing a fully automatic biochemical analyser to determine the absorbance difference after reaction; and calculating the concentration of glycosylated hemoglobin in the sample according to an absorbance change value. The kit provided by the invention has the advantages of high accuracy, and convenient operation, etc.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring glycosylated hemoglobin and a preparation method thereof. Background technique [0002] Glycated hemoglobin is the product of the combination of hemoglobin and blood sugar in red blood cells in human blood. The combination of blood sugar and hemoglobin to form glycosylated hemoglobin is an irreversible reaction and is proportional to the blood sugar concentration, and it remains for about 120 days, so the blood sugar before 120 days can be observed The English code of glycosylated hemoglobin is HbA1c, and the test of glycosylated hemoglobin can usually reflect the blood sugar control of patients in the past 8 to 12 weeks. [0003] Native (non-glycated) hemoglobin is A0 (2α, 2β chains), and subcomponents (HbA1a1, HbA1a2, HbA1b, and HbA1c) are formed by glycosylation of the free amino group of the N-terminal valine of the hemoglobin β chain-N-...

Claims

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Application Information

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IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 蔡晓辉庄庆华吴铮徐运
Owner ANHUI IPROCOM BIOTECH CO LTD
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