Coherent anti-stokes Raman microscopy imaging system generated on basis of double-soliton pulses

Abstract

Provided is a coherent anti-stokes Raman microscopy imaging system generated on the basis of double-soliton pulses. The microscopy imaging system comprises an optical fiber laser for generating original femtosecond pulses, a double-soliton pulse generation and spectrum expansion unit for generating the double-soliton pulses, an optical pulse scanning unit for achieving pulse time-domain scanning, a power ratio control unit for adjusting the ratio of the double-soliton pulses entering the double-soliton pulse generation and spectrum expansion unit and the optical pulse scanning unit, a microscopy imaging system for conducting focusing excitation and signal photon collection on a sample to be detected and a part for conducting detection, extraction process and microscopic image display on signal photons. According to the coherent anti-stokes Raman microscopy imaging system, the double-soliton pulses which have the time domain capable of being distinguished and have frequency differences are obtained on the basis of a full optical fiber system, an excitation light source system is simplified, frequency modulation resonance excitation is achieved, the influence of non-resonant signals on a coherent anti-stokes spectrum is eliminated, and the contrast ratio of coherent Raman microscopy images is significantly increased.

Description

technical field [0001] The invention belongs to the technical field of nonlinear optical microscopic imaging, in particular to a coherent anti-Stokes Raman microscopic imaging system based on double-soliton pulse generation. Background technique [0002] Coherent anti-Stokes Raman Scattering (CARS) is a third-order nonlinear optical process that enables resonant excitation and coherent detection of molecular vibrational levels. Generally speaking, the signal strength of CARS is 10 times higher than that of ordinary Raman processes. 4 -10 5 At the same time, compared with fluorescence microscopy, the CARS signal comes from the intrinsic properties of the molecule and does not need to introduce any external labels, so the CARS technology can achieve label-free, high-sensitivity, and three-dimensional imaging; in the fast and dynamic life sciences important in research. However, while CARS signals are generated, non-resonant signals that do not contain molecular vibration en...

AI Technical Summary

Problems solved by technology

This greatly increases the complexity and detection cost of the CARS light source; at the same time, solid-state pulsed lasers are extremely sensitive to environmental changes, making it difficult to ensure a stable imaging system

In addition, fast wavelength tunability is difficult to achieve in solid-state lasers

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