Microbial organic fertilizer and preparation method thereof

A microbial organic fertilizer and biological technology, applied in the directions of organic fertilizers, fertilization devices, fertilizer mixtures, etc., can solve the problems of agricultural ecological environment pollution, cost increase, pesticide residues, etc., to reduce environmental pollution problems, production costs, and application. effect of times

Inactive Publication Date: 2016-12-14
JIANGXI TIANREN ECOLOGY
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Problems solved by technology

However, the long-term use of chemical pesticides not only greatly increases the cost of planting, but also the residues of chemical pesticides not only endanger the quality of crops and human health, but also cause pests to be resistant to chemical drugs, and also cause pollution to the agricultural ecological environment , not conducive to green environmental protection
[0003] Root-knot nematode is one of the major pathogens of food, vegetables, and economic crops in my country, and it is also the most harmful to crops. These pests and diseases have seriously affected the growth of crops, and at the same time caused economic losses to growers.
At present, chemical pesticides are often used to prevent and control underground pests such as root-knot nematodes, mole crickets, grubs, and needleworms, and plant diseases such as root rot and blight. However, the use of chemical pesticides not only increases the cost of planting, but also has pesticides. Residues will also make root-knot nematodes, mole crickets, grubs, needleworms and other underground pests and plant diseases such as root rot and blight resistant to chemical pesticides

Method used

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  • Microbial organic fertilizer and preparation method thereof

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preparation example Construction

[0035] In the present invention, the preparation method of said H. chlorogenes preferably comprises the following steps:

[0036] 1) activating the H. chlorogenic strain;

[0037] 2) inoculating the activated H. chlorogenes in the primary liquid culture medium of H. chlorogenes for primary liquid culture, and stopping the cultivation after 35 to 50 hours of cultivation to obtain the primary culture medium of H. chlorogenes;

[0038] 3) inoculate the primary culture solution of H. chlorogenicum obtained in the step 2) into the secondary liquid medium of H. chlorogenicum for secondary liquid culture, and stop culturing for 50-75 hours to obtain H. chlorogenicum. Bacteria secondary culture medium;

[0039] 4) Inoculate the secondary culture solution obtained in step 3) into the solid culture medium of H. chlorogenes, and carry out solid fermentation culture to obtain H. chlorogenes.

[0040] In the present invention, the activation of the H. chlorogenic strain is preferably to ...

Embodiment 1

[0084] Preparation of H. chlorogenes: inoculate preserved H. chlorogenes strains into eggplant-shaped bottles equipped with nutrient agar medium, and culture them at 30°C for 35 hours for activation culture; the activated H. chlorogenes cultured with 20% of the inoculum was inoculated in the primary liquid medium after sterilized at 121°C for 35 minutes. The specific culture conditions were: temperature 28°C; pH value 6.0; within 24 hours after inoculation, the ventilation volume was 15m 3 / h, after 24 hours of cultivation, the ventilation rate is 25m 3 / h; Cultivate after 45h and stop cultivating to obtain the first-level liquid culture solution of H. The culture conditions are as follows: the temperature is 30°C; the pH value is 6.0; within 24 hours after inoculation, the ventilation volume is 150m 3 / h, after 24 hours of cultivation, the ventilation rate is 240m 3 Cultivate after 65h, stop culturing, obtain the secondary liquid culture solution of H. chlorogenicum; inocul...

Embodiment 2

[0088] Preparation of H. chlorogenes: inoculate preserved H. chlorogenes strains into eggplant-shaped bottles equipped with nutrient agar medium, and culture them at 20°C for 55 hours for activation culture; 10% of the inoculum was inoculated in the primary liquid medium after sterilized at 121°C for 35 minutes. The specific culture conditions were: temperature 32°C; pH value 5.5; within 24 hours after inoculation, the ventilation rate was 10m 3 / h, after 24 hours of cultivation, the ventilation rate is 15m 3 / h; Cultivate after 50h and stop cultivating to obtain the first-level liquid culture solution of H. chlorogenicum; the first-level culture solution obtained is inoculated in the second-level liquid culture solution of H. The culture conditions are: temperature is 35°C; pH value is 6.5; within 24 hours after inoculation, the ventilation volume is 100m 3 / h, after 24 hours of cultivation, the ventilation rate is 200m 3 Cultivate after 50h, stop cultivating, obtain the se...

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Abstract

The invention provides a microbial organic fertilizer which comprises mature green hypocrea and rotten fertilizer according to the volume ratio of 1:(10-50), and the effective spore amount of mature green hypocrea in the microbial organic fertilizer is 2.0*108 cfu/g or above. The microbial organic fertilizer has a high prevention and treatment effect on plant diseases and insect pests, underground injurious insects such as root-knot nematode, mole cricket, grub and wireworm and plant diseases such as root rot and damping off are reduced, the microbial organic fertilizer can replace part of chemical pesticides for use, the application frequency of chemical pesticides is reduced, and production cost is reduced for farmers. By means of mature green hypocrea, the growth of root systems and plants can be promoted, the growth of plant vegetative organs is promoted, and the yield of crops is increased. The microbial organic fertilizer can not cause drug resistance and can reduce the environmental pollution problem caused by chemical fertilizer and chemical pesticides after being used for a long time.

Description

technical field [0001] The invention relates to the technical field of bio-organic fertilizers, in particular to a microbial organic fertilizer containing H. chlorogenes and a preparation method thereof. Background technique [0002] China is a large agricultural country. With the rapid growth of China's population, the demand for crops is also increasing. However, the yield and quality of crops are often significantly reduced due to the occurrence of diseases and insect pests during the planting process. In order to ensure that the crops can go on the market smoothly and meet the needs of the people, the growers have long used a large amount of chemical pesticides to prevent and control the occurrence of diseases and insect pests, so as to ensure the yield and quality of the crops. However, the long-term use of chemical pesticides not only greatly increases the cost of planting, but also the residues of chemical pesticides not only endanger the quality of crops and human he...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/02C05G3/60
CPCC05F9/04C05G3/00C05G3/60
Inventor 石峥李肖宇曾升华梁小文石仕福吴俊杰罗建辉李英武罗双燕王根豪严艺波
Owner JIANGXI TIANREN ECOLOGY
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