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Alternative Diektella for preventing and treating bacterial wilt and its application

A technology of Dickella and bacterial wilt, applied in the field of agricultural biology, can solve problems such as no research report on Dickella

Active Publication Date: 2019-07-09
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no research report on the use of D. dantidioides as a biocontrol agent for bacterial wilt

Method used

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  • Alternative Diektella for preventing and treating bacterial wilt and its application
  • Alternative Diektella for preventing and treating bacterial wilt and its application
  • Alternative Diektella for preventing and treating bacterial wilt and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: Bacteria carry out separation and purification

[0021] 1. Medium

[0022] LB agar medium (LB solid medium): peptone 10g, yeast extract 5g, NaCl 10g, agar 15g, with H 2 Dilute to 1000mL with O, pH 7.0-7.2. Sterilize at 121°C for 20 minutes.

[0023] 2. Experimental steps

[0024] (1) Sample collection: Select a representative location (root soil of healthy plants in a field with bacterial wilt disease, location in Guangdong Province), shovel off the topsoil, and use soil picking tools to collect a soil layer at a depth of 5 to 10 centimeters below the surface 5 samples were taken from each site, packed into bags and recorded.

[0025] (2) Preparation of soil dilution: Weigh 10 g of soil, put it into a conical flask filled with 90 mL of sterile water and oscillate fully to prepare a soil dilution with a concentration of 1:10. After the soil particles have settled, absorb 1mL of the supernatant and transfer it into a test tube filled with 9mL of steriliz...

Embodiment 2

[0028] Embodiment 2: the plate antagonism test of isolated bacteria to R. solanacearum

[0029] 1. Medium

[0030] TTC solid medium: ① 10g of peptone, 1g of casein acid hydrolyzate (Casein Acid Hydrolysate), 5g of glucose, 15g of agar, with H 2 O was adjusted to 1000mL, pH6.8-7.2; sterilized at 121°C for 20 minutes to obtain solution I; Filter and sterilize with a 0.22 μm filter membrane to obtain solution II; ③ When solution I is cooled to about 45°C, add 0.5mL solution II to every 100mL solution I, and pour the plate.

[0031] TTC liquid medium: do not add agar and TTC.

[0032] 2. Experimental steps

[0033] 2.1 Plate Antagonism Test - Primary Screening

[0034] 2.1.1 Strain activation

[0035] Ralstonia solanacearum and bacterium carry out strain activation: solanacearum GMI1000 (ATCC) and the 384 strains of bacteria isolated in Example 1 were transferred to TTC solid medium plate and LB solid medium plate respectively, and were cultured at 28° C. for 2 days And incu...

Embodiment 3

[0052] Embodiment 3: the biological control effect test of bacterial strain GZ-39

[0053] In order to test the biocontrol effect of the strain GZ-39, the fermentation liquid of the strain GZ-27 was tested on the biocontrol effect of bacterial wilt on potted plants.

[0054] Experimental steps:

[0055] 1.1 Buy eggplant seedlings

[0056] Buy eggplant seedlings grown in greenhouses.

[0057] 1.2 Seedling transplanting

[0058] Fill the flowerpots (size 170mm*160mm) with matrix soil, compact them slightly, and then move one eggplant seedling into each pot, press the roots slightly with two fingers after watering, and cultivate them in a greenhouse. Cultivate in pots for about 4 weeks.

[0059] 1.3 Ralstonia solanacearum and antagonistic bacteria activation and fermentation broth preparation

[0060] Ralstonia solanacearum GMI1000 and antagonistic bacteria GZ-39 were activated respectively, and then single colonies were respectively inserted into TTC liquid medium and LB liqu...

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Abstract

The invention discloses a strain of danditidickia strain used for preventing and treating bacterial wilt and its application. The name of the strain is Dickeya dadantii (Dickeya dadantii) GZ‑39, the preservation number is CCTCC NO: M2016354, and it was preserved on June 27, 2016 in the Chinese Type Culture Collection Center located at Wuhan University, Wuhan, China. The bacterial strain of the invention has significant antagonism to R. solanacearum, and provides another approach for the prevention and treatment of R. solanacearum.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a dactidia strain used for preventing and treating bacterial wilt and its application. Background technique [0002] Plant bacterial wilt is a destructive soil-borne bacterial disease of vascular bundles caused by Ralstonia solanacearum. It is a devastating soil-borne disease with widespread systemic infection in subtropical, tropical and temperate regions. , commonly known as "plant blast". R. solanacearum has a wide range of hosts and can infect more than 450 species of plants in 54 families. At present, the prevention and control of bacterial wilt is a major problem recognized in the world. It is widely distributed all over the world, and it occurs seriously in southern provinces and cities in my country, especially in Zhejiang. In recent years, not only Solanaceae and melons have been damaged, but also large-scale Mulberry bacterial wilt has caused serious losses to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 邓音乐宋施豪贺飞黄小容孙秀云蔡灵芝
Owner SOUTH CHINA AGRI UNIV