Inducible c-myc over-expressed transgenic vector, transgenic mouse model and construction method of transgenic mouse model

Abstract

The invention discloses an inducible c-myc over-expressed transgenic vector, a transgenic mouse model and a construction method of the transgenic mouse model. The construction method comprises the following steps: connecting a c-myc encoding area with a pcDNA3 vector so as to obtain pcDNA3-c-myc plasmid; connecting a Loxp-Stop-Loxp fragment with the pcDNA3-c-myc plasmid so as to obtain pcDNA3-Loxp-Stop-Loxp-c-myc plasmid; connecting an EF1a promoter fragment with the pcDNA3-Loxp-Stop-Loxp-c-myc plasmid so as to obtain a transgenic vector, and constructing the transgenic mouse model on the basis of the transgenic vector. The transgenic mouse model disclosed by the invention is an inducible leukemia model, c-myc is not expressed and no disease signs in the absence of an inducer, c-myc is over-expressed in the presence of the inducer, leukemia can be induced, the transgenic mouse model is relatively good in controllability as compared with a common transgenic mouse model, and the population quantity to be maintained can be adjusted according to experimental requirements.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, more specifically, the invention relates to an inducible c-myc transgene carrier in the hematopoietic system and a construction method thereof, and an inducible c-myc transgenic mouse model in the hematopoietic system and its construction method. Background technique [0002] Leukemia is a malignant disease that occurs in hematopoietic organs and is characterized by abnormal proliferation and development of white blood cells and their precursor cells in blood and bone marrow. According to the report of the World Health Organization, about 210,000 people died of leukemia in the world in 2000, and 90% of them were adults. The characteristics of the mouse hematopoietic system are similar to those of humans, and mouse leukemia is very similar to human leukemia in many ways. The research and development of mouse leukemia model has become a powerful tool for studying human leukemia pathog...

AI Technical Summary

Problems solved by technology

[0006] However, these models also have their limitations: In the case of the Ig heavy chain enhancer-driven c-myc transgenic mouse (Eμ-myc), overexpression of c-myc begins in the embryo and can be observed in postnatal mice Leukemic symptoms such as increased B cell volume have hindered the understanding of the function of the myc gene in the leukemogenesis process caused by c-myc. In this transgenic mouse model, the sustained high expression of myc from the early stage led to The average lifespan is only 12 weeks, and a large population needs to be maintained during the study

[0007] In summary, since the currently commonly used c-myc transgenic mouse model of leukemia has defects such as sustained high expression of c-myc and uncontrollable onset time, it is urgent to establish an inducible spontaneous leukemia transgenic model

Images