Modified ITO electrode for improving electricity production efficiency of electricity producing bacteria and preparation method thereof

A technology of electrogenic bacteria and electrical efficiency, applied in the field of bioelectrochemical sensors, which can solve the problems of electrodes affecting electron transfer efficiency

Active Publication Date: 2016-12-14
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it should be noted that the material and properties of the electrode will also affect its electron transfer efficiency.

Method used

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  • Modified ITO electrode for improving electricity production efficiency of electricity producing bacteria and preparation method thereof
  • Modified ITO electrode for improving electricity production efficiency of electricity producing bacteria and preparation method thereof
  • Modified ITO electrode for improving electricity production efficiency of electricity producing bacteria and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1I

[0031] The modification of embodiment 1ITO electrode

[0032] (1) Modification of phage

[0033] 1. The plate culture of phage is carried out on a double-layer plate, and the formula is as follows:

[0034]

[0035] SolA solution was sterilized at 121° C. for 20 minutes and solB was added to prepare a 1.5% medium. Similarly, if the amount of agar added was 7.6 g, a 0.8% medium was prepared. First pour 1.5% culture medium on the bottom of the plate, after it cools and solidifies, then mix the M13 bacterial solution with 0.8% culture medium and pour it into the upper part of the plate, after solidification, invert the plate, overnight at 30°C, and grow the next day Plaque. Then pick the colony in the LB medium of E.coil, shake it overnight at 30°C to obtain the amplified M13 phage, and its concentration can be obtained by reading the ultraviolet absorption at 269nm (the extinction coefficient is 3.84). In the experiment, it was measured as 140mg / ml.

[0036] 2. Prepare a ...

Embodiment 2M13

[0049] The connection of embodiment 2M13 phage directly with ITO

[0050] Mix 10ul of M13 phage solution with 990ul of 80% PBS, spread evenly on the ITO glass slide, and carry out the measurement of the electrochemical experiment, the steps are the same as above

Embodiment 3 2

[0051] The connection of embodiment 3 ferrocene formaldehyde and ITO

[0052] Take 700ul of the ferrocene formaldehyde solution in step 2 of Example 1, mix it with 400ul of the solution in step 3, react at room temperature for 1h, and carry out the experiment according to the previous steps.

[0053] All experimental results are as follows:

[0054] image 3 It can be seen that among the three modified ITO electrodes, M13 and ferrocene mixed solution have the best electrical signal, and the I-t image has an obvious peak, reaching 35uA,, and when only M13 or ferrocene is connected , the current is basically unchanged and maintained within 5uA. It can be seen that the modified electrode can promote the adsorption of Shewanella, thereby facilitating its electron transfer. The reason may be that the virus promotes the adsorption of electrogenic bacteria, and then transfers electrons to the ITO electrode through ferrocene to generate electrical signals. When there is only M13 o...

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Abstract

The invention discloses a modified ITO electrode for improving electricity production efficiency of electricity producing bacteria and a preparation method thereof. The method comprises the following steps: reacting the amine group on a protein shell of M13 bacteriophage with ferrocene formaldehyde for connection, and uniformly coating the surface of ITO; assembling a three-electrode reactor and conducting electrochemical parameters scanning by an electrochemical workstation; and using the modified electrode as a working electrode, Ag / AgCl as a reference electrode and platinum wire as a negative electrode. In this environment, the electron transfer efficiency and capacity of the electricity producing bacteria can be accelerated. The electrode modification method is more simple and feasible compared to gene reconstruction on electricity producing bacteria for improvement of electricity producing capability, and has good development space.

Description

technical field [0001] The invention belongs to the field of bioelectrochemical sensors, and in particular relates to a virus-modified electrode to improve the electricity production efficiency of electrogenic bacteria, which can be used to prepare high-precision sensors. Background technique [0002] Electrogenic bacteria refer to a class of microorganisms that can use electrons generated in their own respiratory chain to transfer to external electron acceptors to generate current. Among them, anodic respiration bacteria can extend the electron respiratory chain to the outside of the cell, thereby transferring electrons to extracellular electron acceptors, such as: oxygen, metal oxides (Fe(III)Mn(II)), etc. Due to this property, they have broad prospects in the treatment of pollutants. The most representative ones are Geobacter sulfurreducen and Shewanella oneidensis. The way they transfer electrons mainly includes direct transfer and indirect transfer. Taking Shewanella...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/327
Inventor 张艺馨赵新雷郭明洋迟慧梅
Owner SOUTHEAST UNIV
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