Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

High-throughput nucleic acid aptamer sensor for detecting insulin and preparation method thereof

A nucleic acid aptamer and insulin technology, applied in the field of biosensors, can solve problems such as complex operation and long insulin detection time, and achieve the effects of wide application prospects, real-time detection, and convenient operation

Active Publication Date: 2016-12-21
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
View PDF1 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is that the detection time of insulin in the detection process is long, the operation is complicated, etc., and a high-throughput nucleic acid aptamer sensor for detecting insulin and its preparation method are disclosed

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High-throughput nucleic acid aptamer sensor for detecting insulin and preparation method thereof
  • High-throughput nucleic acid aptamer sensor for detecting insulin and preparation method thereof
  • High-throughput nucleic acid aptamer sensor for detecting insulin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] Insulin-specific aptamer Aptamer in the present invention, sequence such as 5'-SH-(CH 2 ) 6 -ACA GGG GTG TGG GGACAG GGG TGT GGG G-3'shown; the complementary chain sequence is shown as 5'-SH-(CH 2 ) 6 -ACA GCA TTC AGC GCA TCG G-3 'shown; reagents used: PBS buffer, 10mmol / L, PH7.4 (Na 2 HPO 4 , 10mmol / L; KH 2 PO 4 , 2mmol / L; NaCl, 137mmol / L; KCl, 2.7mmol / L); Tris-acetate buffer, 10mmol / L, pH 5.2; TCEP, 2.5mmol / L; mercaptoethanol, 1mmol / L; TCEP and mercaptoethanol Prepared with Tris-acetate buffer. Sucrase: prepared with PBS to 2mg / mL; sucrose, 1mol / L, prepared with PBS; concentrated nitric acid, with a purity of 65%-68%, and a concentration of 16mol / L; the experimental water was ultrapure water with a resistance of 18.2MΩ.

[0022] (1) Preparation of gold nanoparticles and 96 microwell plates

[0023] First, use 50 μL of 16mol / L HNO in a 96-microwell plate 3 Treat for 2 hours, then rinse with double distilled water for 3 times, add 100 μL nano-gold solution (diam...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of biosensors, and in particular, relates to a high-throughput nucleic acid aptamer sensor for detecting insulin and a preparation method thereof. Nano gold particles are immobilized and combined on a micro-porous plate, and the nano gold particles and an insulin nucleic acid aptamer are combined on the micro-porous plate by Au-S bonds; at the same time, sucrase and a DNA complementary strand partially complementary with the insulin nucleic acid aptamer are immobilized on the nano gold particles, the insulin nucleic acid aptamer and the DNA complementary strand form a stable double-strand structure according to a base pairing principle, and a DNA complementary strand / sucrase / nano gold detection probe is prepared; the probe is immobilized to a nano gold micro-porous plate of the insulin nucleic acid aptamer, and the high-throughput nucleic acid aptamer sensor for detecting insulin is obtained. The application potential of a nucleic acid aptamer molecular recognition system in the biomedical field becomes reality, and simple and real-time detection can be really realized in accident spots or other occasions, so the high-throughput nucleic acid aptamer sensor has wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and in particular relates to a high-throughput nucleic acid aptamer sensor for detecting insulin and a preparation method thereof. Background technique [0002] Diabetes is a major metabolic disease that threatens human health and can cause a series of metabolic disorders and complications (such as diabetic ketoacidosis, cardiovascular disease, kidney, eye disease, neuropathy, retinopathy, and diabetic foot). The implementation of blood sugar testing can better control the blood sugar changes of diabetic patients, reduce the risk of diabetic complications, improve the quality of life of patients, and improve their physical condition. The current routine blood sugar detection product is a portable blood glucose meter, which can only detect a single target blood sugar (glucose), and is difficult to apply to the detection of different types of targets, which greatly limits the application range o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68G01N33/53G01N33/66
CPCG01N33/5308G01N33/66G01N33/68G01N2333/62G01N2800/042
Inventor 姜利英闫艳霞张培郑晓婉任林娇陈青华王延峰姜素霞齐汝宾赵志永
Owner ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com