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Formula for enlarging sterilization range and improving sterilization ability of nisin

A nisin, range technology, applied in nisin-related fields, to achieve good degradability, good antibacterial effect, high safety effect

Pending Publication Date: 2017-01-04
AMTECH BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that nisin alone can only inhibit Gram-positive bacteria at present. The present invention makes the compound formula effective against Gram-negative bacteria and fungi by compounding other ingredients. Has a good inhibitory effect

Method used

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  • Formula for enlarging sterilization range and improving sterilization ability of nisin

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specific Embodiment approach 1

[0011] Specific embodiment one: a kind of formula of expanding nisin bactericidal range and improving bactericidal ability of this embodiment, it is the nisin solution that concentration is 0.1~1.0g / L, and concentration is 5.0~50.0g / L Tetrasodium glutamic acid diacetate solution, gluconic acid solution with a concentration of 0.5-5.0g / L, gluconate solution with a concentration of 5.0-50.0g / L, potassium cinnamate solution with a concentration of 0.1-5.0g / L and a solution made of citric acid solution with a concentration of 1.0-10.0 g / L; the pH of the solution is 2.5-7.5.

specific Embodiment approach 2

[0012] Specific embodiment two: the difference between this embodiment and specific embodiment one is that it consists of a nisin solution with a concentration of 0.5 to 1.0 g / L, and tetrasodium glutamic acid diacetate with a concentration of 10.0 to 40.0 g / L. Solution, gluconic acid solution with a concentration of 1.0-5.0g / L, gluconate solution with a concentration of 10.0-40.0g / L, potassium cinnamate solution with a concentration of 1.0-5.0g / L and a solution with a concentration of 2.0-8.0g / L A solution made from 1 L of citric acid solution; the pH of the solution is 2.5-7.5. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0013] Specific embodiment three: the difference between this embodiment and specific embodiment one is that it consists of nisin solution with a concentration of 0.5~0.8g / L, tetrasodium glutamic acid diacetate with a concentration of 20.0~40.0g / L Solution, gluconic acid solution with a concentration of 2.0-5.0g / L, gluconate solution with a concentration of 20.0-40.0g / L, potassium cinnamate solution with a concentration of 2.0-5.0g / L and a solution with a concentration of 2.0-6.0g / L A solution made from 1 L of citric acid solution; the pH of the solution is 2.5-7.5. Others are the same as in the first embodiment.

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Abstract

The invention discloses a formula for enlarging the sterilization range and improving sterilization ability of nisin, relates to the related field of nisin and aims to solve the problems that nisin can only have an inhibition function on gram-positive bacteria when used separately at present. The formula is a solution prepared from a nisin solution, a tetrasodium glutamate diacetate solution, a gluconic acid solution, a gluconate solution, a potassium cinnamate solution and a citric acid solution; the pH of the solution ranges from 2.5 to 7.5. The formula has the characteristics of being safe, economical and practical and is applicable to bacteriostasis and sterilization operation procedures of food, catering, daily chemicals, cultivation and other industries. The formula is widely applied to the field of food, medicine, daily chemicals and the like. Cinnamic acid and potassium cinnamate are novel food additives and have a good bacteriostasis function.

Description

technical field [0001] The present invention relates to the field related to nisin. Background technique [0002] Nisin (English name Nisin), also known as nisin, is a polypeptide substance produced by Streptococcus lactis. It consists of 34 amino acid residues and has a molecular weight of about 3500Da. Because Nisin can inhibit most Gram-positive bacteria, such as Clostridium botulinum, Staphylococcus aureus, Streptococcus hemolyticus, Listeria, and has a strong inhibitory effect on the spores of Bacillus, it is used as a food preservation Agents are widely used in the food industry. Generally, bacteria that produce spores are highly resistant to heat. For example, if fresh milk is sterilized at 135°C for 2 seconds, the mortality rate of non-spore bacteria is 100%, that of spore bacteria is 90%, and that of spore bacteria is 10%. Spore bacteria cannot be killed. If 0.03-0.05g / kg Nisin is added to fresh milk, the germination and reproduction of Bacillus and Clostridium s...

Claims

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Application Information

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IPC IPC(8): A23L3/3571
CPCA23L3/3571
Inventor 梁恒宇张钦革王贵元郭坤马之宵李伟陈傲冰张钦鹏
Owner AMTECH BIOTECH
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