Composition containing cannabis sativa extract and application thereof in cleaning articles
A technology of cleaning products and extracts, which is applied in the direction of surface active non-soap compounds and washing compositions, detergent compositions, organic washing compositions, etc., which can solve the problems of different shapes, inability of anaerobic bacteria to survive, low economic benefits, etc. question
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Embodiment 1
[0067] Embodiment 1 Preparation of hemp extract of the present invention
[0068] method 1:
[0069] 1) Wash and air-dry the raw material cannabis stalks, cannabis flowers and / or cannabis leaves;
[0070] 2) Crush the above-mentioned air-dried raw materials to 10-40 mesh;
[0071] 3) Extract the obtained powder with 4-8 times the amount of 30%-60% ethanol by cold soaking for 1-3 times, each time for 0.5-2 hours;
[0072] 4) The combined extracts are decolorized by 0.1-1w% activated carbon adsorption;
[0073] 5) Concentrate under reduced pressure to a relative density of 1.05-1.30 at a temperature of 70°C;
[0074] That is the hemp extract extract.
[0075] According to the above preparation method,
[0076] When the raw material is hemp stalk core, hemp extract 1A is obtained;
[0077] When the raw material is cannabis flower, the cannabis extract 2A is obtained;
[0078] When the raw material is cannabis leaves, the cannabis extract 3A is obtained;
[0079] When the ...
Embodiment 2
[0099] Example 2 Determination of the total content of cannabinoids in the cannabis extract
[0100] 1. Experimental principle
[0101] Phenol reacts specifically with FoLin-CiocaLteu reagent, and the reaction product has maximum absorption at a specific wavelength, and the absorbance value is linearly related to the amount of phenol within a certain concentration range. First establish a standard curve with standard phenolic substances, and then measure the absorbance of the sample to be tested, based on which the content of polyphenols in the sample to be tested can be calculated.
[0102] 2. Experimental method
[0103] 1. Drawing of standard curve
[0104] 1) Preparation of Folin-Ciocalteu reagent
[0105] Take 1ml of Folin-Ciocalten reagent, add 9ml of distilled water, and mix thoroughly to obtain a 10-fold diluted Folin-Ciocalteu reagent for later use.
[0106] 2) 20%Na 2 CO 3 Preparation of solution (W / V)
[0107] Take Na 2 CO 3 10H 2 O 20g, dissolved in 100ml...
Embodiment 3
[0119] Example 3 Antibacterial Test of Cannabis Extract
[0120] 1. Experimental principle
[0121] Through in vitro antibacterial experiments, the antibacterial effect of the cannabis extract prepared by the present invention on common strains was evaluated with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).
[0122] 2. Experimental method
[0123] Bacteria solution preparation: Pick the slant strains and inoculate them in 20% beef infusion medium, culture overnight at 37°C, then dilute the bacteria solution to 10cfu / mL with 20% bovine infusion medium, and inoculate immediately.
[0124] Determination of MIC: The liquid test tube serial dilution method is used to serially dilute the sample with 20% bovine infusion medium. Add 1.0mL into sterilized test tubes, add 0.05mL standard fresh bacterial solution to each tube, and set up strain control and solvent control groups, incubate at 37°C for 18-24 hours, observe the growth of bacteria, a...
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