Composite microbial inoculum for repairing OCP (Organochlorine Pesticide) polluted soil, preparation method and repairing method

Abstract

The invention provides a composite microbial inoculum for repairing OCP (Organochlorine Pesticide) polluted soil, a preparation method of the composite microbial inoculum for repairing the OCP polluted soil, and a repairing method of the soil by using the composite microbial inoculum. A microbial body of the composite microbial inoculum comprises pseudomonas aeruginosa, pseudomonas putida, stenotrophomonas maltophilia and flavimonas oryzihabitans. Enriched culture is carried out on the strains of pseudomonas aeruginosa, pseudomonas putida, stenotrophomonas maltophilia and flavimonas oryzihabitans, then freeze-drying is carried out to obtain bacterial powder, and the bacterial powder is mixed to obtain the composite microbial inoculum. After the polluted soil is ground, mung bean powder and the composite microbial inoculum are added and the soil is covered with plastic films after plowed, and later plowing is regularly carried out and the content of organochlorine is detected, so that the polluted soil is repaired. The composite microbial inoculum disclosed by the invention has low cost and the four strains are in a mutual synergistic effect, so that the degradation effect of the composite microbial inoculum on OCPs is obviously superior to the sum of the degradation effects of the single strains. The degradation rate of a microflora of the composite microbial inoculum on the polluted soil is much higher than that of the single strains in the repairing process of 45 days under summer natural environment conditions, the degradation rate of the microflora in 15 days can reach more than 20%, and the effect is obvious. Furthermore, the process is simple and the cost is low.

Description

Technical field [0001] The invention relates to the technical field of microbial inoculants for contaminated soil, in particular to a microbial composite inoculum for repairing soil contaminated by organochlorine pesticides, a preparation method and a restoration method. Background technique [0002] Pesticides are important agricultural materials and play an important role in world agricultural production. They play a very important role in preventing and controlling diseases, pests, weeds, and rodents, and ensuring high and stable agricultural yields. Organochlorine pesticides (OrganochlorinePesticides, OCPs), also known as typical persistent organic pollutants, have received widespread attention around the world due to their outstanding persistence, bioaccumulation and biological toxicity. They were applied before the 1980s It is one of the most important and effective pesticide varieties. Because of its low price, high efficiency and broad spectrum, it has been widely used in...

AI Technical Summary

Problems solved by technology

Among them, physical methods and chemical methods, such as incineration and electrochemical methods, generally have disadvantages such as high treatment cost, easy to cause secondary pollution, and poor removal effect, while biological methods mainly use the specific degradation mechanism of microorganisms to OCPs to degrade. The treatment effect of this method is obvious, and it plays an important role in the process of degrading residual pesticides, and has become one of the main means of controlling residual pesticide pollution.

At present, most of the biological methods use a single type of microorganism to specifically degrade OCPs, and the degradation efficiency is low, and no research has shown that composite microorganisms can play a synergistic effect, so a single microorganism is still used to degrade and restore soil

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