Method for developing simple sequence repeats molecular markers of genome of ammopiptanthus mongolicus (Maxim.) Chengf. plant

Abstract

The invention relates to a method for developing SSRs (simple sequence repeats) molecular markers of genome of an ammopiptanthus mongolicus (Maxim.) Chengf. plant, wherein the method comprises the following steps: (1) establishing a primary sequencing library for genome DNA of a species sample sampled from a first production area, and conducting high-throughput sequencing so as to obtain Short Reads sequencing data; (2) filtering Reads obtained from sequencing and conducting sequence assembling so as to obtain Contigs; (3) conducting SSRs identification on Contigs sequences; and (4) verifying the SSRs in a Unigene sequence of a same species derived from a second production area, and conducting screening so as to obtain the SSRs having polymorphism. The high-throughput SSRs molecular marker discovering method provided by the invention is applicable to such researches as genetic map construction, QTL (quantitative trait loci) location, genetic diversity analysis and the like of the plant species.

Description

technical field [0001] The invention relates to a molecular marker method for developing simple repeat sequences (hereinafter referred to as SSR or SSRs, SimpleSequence Repeats, consisting of 1-6 nucleotides, also called microsatellite DNA) of the plant genome of Ilex chinensis, especially in Mongolia A method for molecular markers of the Ilex chinensis genome. Background technique [0002] DNA molecular marker is a kind of genetic marker, which is a marker at the genome level, and can reflect a specific DNA segment of a certain difference in the genome of an individual or population. The number of DNA molecular markers is large, throughout the entire genome, high polymorphism, genetic stability, and not limited by the environment and gene expression. It includes RFLP, RAPD, AFLP, SSRs and ISSR etc. [0003] SSRs (Simple Sequence Repeats) are simple repeating sequences, also known as microsatellite DNA, consisting of 1-6 nucleotides repeated continuously. The same type of ...

AI Technical Summary

Problems solved by technology

However, at present, people have limited understanding of the genome level of A. japonicus, which limits the further application of this excellent germplasm resource.

[0005] Compared with conventional plants such as rice and wheat, the number of existing SSRs markers of Ilex japonica is very small, which cannot meet the needs of research. Therefore, the batch development of SSRs sequences of Ilex japonica is conducive to the construction of genetic maps of Ilex japonica and the mapping of important trait genes.

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