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Immunochromatography kit for fluorescent quantitative detection of hog cholera virus neutralizing antibody

A fluorescent quantitative detection and swine fever virus technology, which is applied in the field of animal medical inspection, can solve the problems of inability to quantify and effectively guide pig production, and achieve fast detection speed, easy operation, and uniform response and signal release.

Inactive Publication Date: 2017-01-04
郑州赛孚生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These existing detection technologies require professional laboratory operators, and it is difficult to popularize and apply them in China's existing breeding scale layout. Although the immune colloidal gold technology is simple and fast, it cannot be quantified and cannot effectively guide pig production

Method used

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  • Immunochromatography kit for fluorescent quantitative detection of hog cholera virus neutralizing antibody
  • Immunochromatography kit for fluorescent quantitative detection of hog cholera virus neutralizing antibody
  • Immunochromatography kit for fluorescent quantitative detection of hog cholera virus neutralizing antibody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Kit

[0029] An immunochromatographic kit for fluorescent quantitative detection of neutralizing antibodies to classical swine fever virus, the structure of which is as follows: figure 1 with figure 2 As shown, it consists of a single-layer test strip 11 and a cartridge. The cartridge is composed of a cartridge upper cover 10 and a cartridge lower cover 12. A sample injection hole 7 and a display window 8 are provided on the cartridge upper cover 10. Said sample loading hole 7 is located above the sample pad 1, said display window 8 is located directly above the detection pad 3, and the size of the display window 8 is the same as that of the detection pad 3; said test strip 11 consists of the sample pad 1. The marker pad 2, the detection pad 3, and the water-absorbing paper 5 are sequentially lapped and pasted on the polyethylene plastic base plate 6 to form, and a detection belt 4 is arranged on the detection pad 3; the marker pad 2 contains an even Fluore...

Embodiment 2

[0033] Embodiment 2: preparation method

[0034] The sources of raw materials used in the preparation of the immunochromatographic kit for fluorescent quantitative detection of CSFV neutralizing antibody are as follows:

[0035] Glass fibers, polyester fibers, nitrocellulose membranes, cotton pulp boards, and PVC membranes were all purchased from Shanghai Jinbiao Biotechnology Co., Ltd.; card shells were purchased from Cangzhou Shengfeng Plastic Products Co., Ltd.; fluorescent immune microspheres were purchased from Nanjing Weicei Biotechnology Co., Ltd. Technology Co., Ltd.

[0036] The preparation method of the immunochromatography kit for fluorescent quantitative detection of classical swine fever virus neutralizing antibody comprises the following steps:

[0037] (1) Microsphere activation

[0038] 1.a. Take 200 μL of fluorescent immune microspheres with a size of 100 nm into a 1.5 mL centrifuge tube, centrifuge and wash once with an equal volume of morpholine ethanesulfon...

Embodiment 3

[0045] Embodiment 3: preparation method

[0046] The sources of raw materials used in the preparation of the immunochromatographic kit for fluorescent quantitative detection of CSFV neutralizing antibody are as follows:

[0047] Glass fibers, polyester fibers, nitrocellulose membranes, cotton pulp boards, and PVC membranes were all purchased from Shanghai Jinbiao Biotechnology Co., Ltd.; card shells were purchased from Cangzhou Shengfeng Plastic Products Co., Ltd.; fluorescent immune microspheres were purchased from Nanjing Weicei Biotechnology Co., Ltd. Technology Co., Ltd.

[0048] The preparation method of the immunochromatography kit for fluorescent quantitative detection of classical swine fever virus neutralizing antibody comprises the following steps:

[0049] (1) Microsphere activation

[0050] 1.a. Take 100 μL of carboxyl-modified fluorescent immune microspheres with a size of 200 nm into a 1.5 mL centrifuge tube, wash twice with an equal volume of morpholine ethane...

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Abstract

The invention discloses an immunochromatography kit for fluorescent quantitative detection of hog cholera virus neutralizing antibody. The immunochromatography kit comprises a monolayer test strip and a shell; the shell is composed of an upper cover of the shell and a lower cover of the shell; a sampling hole and a display window are arranged in the upper cover of the shell, the display window is positioned above a nitrocellulose coating film, and the size of the display window is the same as that of the nitrocellulose coating film; the test strip is formed by orderly overlapping and bonding a sample cushion, a marker cushion, the nitrocellulose coating film, and water absorbing paper on the bottom plate of a polyethylene plastic bottom plate, and a detecting strip is arranged on the nitrocellulose coating film; the marker cushion contains a fluorescent immunoassay microspheres coupled with hog cholera virus E2 protein; the detecting strip is coated with the hog cholera virus E2 protein. The kit provided by the invention has the advantages of high sensitivity, high specificity, and the ability to perform exact quantification in conjunction with a reader.

Description

technical field [0001] The invention belongs to the field of animal medical inspection, and relates to an immunochromatographic kit for fluorescent quantitative detection of swine fever virus neutralizing antibody. Background technique [0002] Swine fever is an important infectious disease in my country's pig industry. It belongs to a class of animal diseases and has caused serious harm to my country's pig industry. The economic loss caused to my country is nearly 10 billion yuan every year, and it is the disease that causes the largest economic loss in my country's pig industry. Due to its harmfulness and extensiveness on a global scale, scholars from all over the world have conducted a lot of research in order to establish a rapid, accurate, highly sensitive, easy-to-operate and quantitative detection technology for neutralizing antibodies to CSFV. [0003] Common related detection techniques include: virus isolation and identification, chicken Newcastle disease virus en...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558G01N33/543
CPCG01N33/56983G01N33/54313G01N33/558G01N2333/08G01N2469/20
Inventor 崔峥赵森林冯现明董海岚单留江陈海洲张瑞李芳李玉青杨盼盼李廷军
Owner 郑州赛孚生物工程有限公司