Kit for determining retinol binding protein and testing method of kit

Abstract

The invention discloses a kit for determining retinol binding protein (RBP) content through a magnetic particle chemiluminiscence method. The kit comprises a calibrator, a quality controller, resistance reagents, a magnetic particle reagent and a light-emitting substrate; the resistance reagents are a retinol binding protein coated antibody labeled with fluorescein isothiocyanate and a retinol binding protein labeled antibody labeled with alkaline phosphatase; the magnetic particle reagent is a magnetic particle and goat antibody FITC connector. The chemiluminiscence technology and immune magnetic particles are combined, and an approximately homogeneous reaction system is provided; by the adoption of a one-step reaction pattern, detection sensitivity and precision are greatly improved, the detection range is expanded, reaction time is greatly shortened, less than 25 min is spent from sample addition to result detection, and the kit is obviously faster than a similar kit; multiple samples can be tested on fully-automatic chemiluminescence apparatus at the same time, high-flux rapid determination of retinol binding protein is realized, accuracy is high, specificity is high, and precision and detection efficiency are greatly improved.

Description

technical field [0001] The invention relates to the technical field of biochemistry, in particular to a kit for measuring the content of retinol-binding protein (RBP) in a human body by a magnetic particle chemiluminescence method and a testing method thereof. Background technique [0002] Retinol-binding protein (RBP) is mainly synthesized by liver cells and widely distributed in human serum, cerebrospinal fluid, urine and other body fluids. In the blood, RBP exists in the form of a 1:1:1 (mol) complex with retinol and prealbumin, transporting 90% of retinol in the body to body tissues, when RBP binds to the RBP receptor on the cell surface When retinol enters the cell, the complex disintegrates, and the free RBP is filtered out from the glomerulus, most of which are reabsorbed by the proximal renal tubular epithelial cells and decomposed for tissue utilization, only a small amount is released from the urine discharge. Diseases such as zinc and iron deficiency and severe ...

AI Technical Summary

Problems solved by technology

The steps of radioimmunoassay are cumbersome, the reagents are expensive, and supporting equipment is required and there is radioactive contamination

Enzyme-linked immunosorbent assay has long detection time, complicated operation, poor repeatability, and is not suitable for emergency and clinical patients in time for diagnosis.

Latex-enhanced immunoturbidimetric method is simple and fast to operate, but has low sensitivity and poor reproducibility at low values

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